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Showing: 341 - 360 of 408 results

  1. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type
    Blog Post
    ...Konermann, the first generation of CRISPR-Cas9 plasmids were not designed with gene activation in mind...tetraloop and stem-loop 2; 3. The MS2-P65-HSF1 plasmid which expresses the activation helper protein. ...Nureki O. Cell. 2014 Feb 27;156(5):935-49. doi: 10.1016/j.cell.2014.02.001. Epub 2014 Feb 13. Genome-scale...Weissman JS. Cell. 2014 Oct 23;159(3):647-61. doi: 10.1016/j.cell.2014.09.029. Epub 2014 Oct 9.     ...

  2. Addgene's eBook Collection

    Type
    Guide
    ... Download Select Plasmids 101 September 2023 | Fourth Edition About Plasmids 101 September 2023 ...popular Plasmids 101 blog series, this eBook covers everything you need to know about plasmids, from parts...About Overlay Select CRISPR 101 January 2021 | Third Edition About CRISPR 101 January 2021 | Third...Fluorescent Proteins 101 August 2017 | First Edition About Fluorescent Proteins 101 August 2017 | ... Select Viral Vectors 101 July 2024 | Second Edition About Viral Vectors 101 July 2024 | Second... Select CRISPR 101 en español January 2024 | First Edition, trans. About CRISPR 101 en español January...nuestro libro electrónico, CRISPR 101. Close About Overlay Select Antibodies 101 New! October 2024 | First...

  3. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    ... [6], plasmid electroporation (episomal) [7, 8] and mRNA transfection [9]. Many of the plasmids used for...Neurons [32, 33, 34] You can find a variety of plasmids for differentiation here. Transdifferentiation...Pages Find Viral Vectors for Your Research Find Plasmids from the Yamanaka Lab ... PubMed PMID: 24291815. PubMed Central PMCID: PMC4101921. 19. Dias, J., et al., Generation of red blood... PubMed PMID: 21434814. PubMed Central PMCID: PMC3161101. 20. Chang, C.J., et al., Production of embryonic...

  4. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    ...the upstream recombination outcome. Find SPARC plasmids at Addgene To more precisely control the amount...Resources on Addgene.org Find fluorescent protein plasmids at Addgene Read our Molecular Biology Reference...proportions of cells. bioRxiv 788679; https://doi.org/10.1101/788679 Lin R, Wang R, Yuan J, Feng Q, Zhou Y, Zeng...

  5. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    ... allows us to provide scientists with complete plasmid sequences. After launching our viral service in... the packaging cell line, helper and packaging plasmids. We decided to use this to our advantage by designing...Molecular Therapy 25:1375–1386 . https://doi.org/10.1016/j.ymthe.2017.04.001 Additional resources on the...

  7. Deep Dive: qPCR

    Type
    Blog Post
    ...Introduction to PCR.) But if you're ready to go beyond the 101 and take your understanding to the next level, read..., Pages 19-28, ISSN 2214-7535, https://doi.org/10.1016/j.bdq.2017.11.001.Bustin SA; Benes V; Garson JA...Bioinformatics, Volume 27, Issue 7, 1 April 2011, Pages 1019–1020, https://doi.org/10.1093/bioinformatics/btr065Freeman... Pages 761-774, SSN 0167-7799, https://doi.org/10.1016/j.tibtech.2018.12.002.Teo IA; Choi JW;  Morlese...Addgene blog Digital Droplet PCR for AAV QuantificationPlasmid Cloning by PCR...

  8. Educational Resources

    Type
    Guide
    .... Titles include: Plasmids 101 CRISPR 101 Viral Vectors 101 Fluorescent Proteins 101 Science Career Guide...Viral Vectors Plasmid Technology Career Readiness Molecular Biology Learn about plasmids, sequencing, ...biology, plasmid cloning, and virus, including: Gel Electrophoresis Bacterial Transformation Plasmid Cloning...include: Protocols Careers How-to Blog Read about plasmids in Addgene’s repository , and related molecular...Concentrations for Bacterial Selection What is a Plasmid? Genetic Code Strain Information Molecular Cloning...

  9. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...results in as little as an hour. Find the SHERLOCK plasmids at Addgene! Applications of the SHERLOCK detection...sensitivity from patient samples. Find the DETECTR plasmids here! Applications of DETECTR in diagnostics DETECTR...Systems. Molecular Cell 60:385–397 . https://doi.org/10.1016/j.molcel.2015.10.008 Zetsche B, Gootenberg JS, ...CRISPR-Cas System. Cell 163:759–771 . https://doi.org/10.1016/j.cell.2015.09.038 Additional resources on the ...

  10. Promoters

    Type
    Guide
    ...biology basics and plasmid components. Plasmids 101: The Promoter Region-Let's Go Plasmids 101: Inducible Promoters...Promoters Plasmids 101: Repressible Promoters Plasmids 101: Terminators and PolyA signals... popular Plasmids 101 series , covers topics ranging from the newest breakthroughs in plasmid technologies... Plasmid Reference Molecular Biology Reference Promoters...

  11. Cloning

    Type
    Guide
    ...of interest (YGOI) into a recipient plasmid. (Image from Plasmid 101: Restriction cloning ) Back to Top...Recombination Cloning Gateway cloning (Image from Plasmid 101: Gateway Cloning ) Gateway® cloning is a recombination.... Back to Top TOPO® Cloning TOPO (Image from Plasmid 101: TOPO Cloning ) Toposiomerase based cloning, ...product into a plasmid. The major disadvantage of TOPO® cloning is that very few plasmid backbones are ...Entry plasmids are also available, allowing for rapid cloning of PCR products into donor plasmids without... For more information and tips check out our Plasmid 101 blog post on TOPO cloning. Back to Top Gibson... 5' exonuclease. Gibson Assembly (Image from Plasmid 101: Gibson Assembly ) Back to Top Type IIS Assembly...

  12. Molecular Biology Reference

    Type
    Guide
    ...Restriction Cloning Plasmids 101: Inducible Promoters Plasmids 101: What is a plasmid? Plasmids 101: Antibiotic...biology basics and plasmid components. Plasmids 101: Origin of Replication Plasmids 101: The Promoter Region-Let...Reference Guide Plasmids 101: 5 factors to help you choose the right cloning method Plasmids 101: Restriction...Molecular Genetics Plasmid Elements Working with Plasmids Introduction Types of Plasmids E. coli strains ...propagating plasmids Antibiotics commonly used for plasmid selection DNA sequencing for plasmid verification...to be studied into the plasmid. Below are some common plasmid elements: Plasmid Element Description Origin...our popular Plasmids 101 series covers topics ranging from the newest breakthroughs in plasmid technologies...

  13. A History of Genome Engineering in Popular Culture

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    Blog Post
    ... our CRISPR guide Find CRISPR plasmids for your research Browse plasmids for base editing References ...Journal of Hygiene 27:113–159 . https://doi.org/10.1017/s0022172400031879 Hershey AD, Chase M (1952) INDEPENDENT...Sciences de la Vie 323:1053–1060 . https://doi.org/10.1016/s0764-4469(00)01250-6 Ran FA, Hsu PD, Wright J,...

  14. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...user Resources on Addgene.org Find Adenovirus Plasmids Adenovirus Guide Page Subscribe to Viral Vectors...to ~1x1014 vg/ml.. As evident in our repository, this application is an outlier for PHP.eB (1x1011 - 1x1012... 1x1012 vg/mouse is more standard) and reflects a desire for very high MOI on top of very high coverage...

  15. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...freezer, you’ll probably find lots of different plasmids containing the same protein of interest tagged...Addgene’s collection, so you may be able to get a plasmid ready-to-use without any cloning at all! As mentioned...Chemical Biology, 26(4), 584-592.e6. https://doi.org/10.1016/j.chembiol.2019.01.003 Gallagher, S. S., Sable,...Chemistry & Biology, 15(2), 128–136. https://doi.org/10.1016/j.chembiol.2008.01.007 Griffin, B. A., Adams, S...

  16. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    .... elegans. These modular toolkits build single plasmid targeting vectors that encode the gRNA transcript... C. elegans, visit Addgene’s C. elegans CRISPR plasmids page. Future perspectives It is interesting to...Precision Genome Editing. BioRxiv. https://doi.org/10.1101/354480 Ghanta KS, Mello CC (2020) Melting dsDNA...elegans. Methods 121–122:86–93 . https://doi.org/10.1016/j.ymeth.2017.03.023 Paix A, Wang Y, Smith HE, Lee...

  17. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...get right. Here at Addgene, we use a linearized plasmid standard. However, even when the standard is made... could end up with a titer of 4 x 1012 genome copies/mL or 8 x 1012 genome copies/mL and both results ... Since AAV titers tend to be in the range of 1012 to 1013 GC/mL, you must serially dilute your virus before...

  18. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ... Resources on Addgene.org Fluorescent Protein Plasmids and Resources Fluorescent Protein Guide: Biosensors... Autophagy Research. Cell,140(3), 313-326. doi:10.1016/j.cell.2010.01.028. PubMed PMID: 20144757. PubMed... and Non-Mammalian Systems, Part A, 33-42. doi:10.1016/s0076-6879(08)03203-5. PubMed PMID: 19185711. Additional...

  19. RNA Extraction Without A Kit

    Type
    Blog Post
    ...on the Addgene blog Read blog posts related to plasmid protocols Find protocols for purifying DNA without...BBA) - Enzymology 341:484–488. https://doi.org/10.1016/0005-2744(74)90240-x Chirgwin JM, Przybyla AE, ...Analytical Biochemistry 162:156–159. https://doi.org/10.1016/0003-2697(87)90021-2 Chomczynski P, Sacchi N (2006...
Showing: 341 - 360 of 408 results