...The undigested (-, lane 1) DNA duplex and those formed from control (GFP gRNA-targeted) BRAF sites (lanes...what I think are the main tips for each step of performing and analyzing a gene edit using Addgene’s lentiviral...lentiCas9-blast. Anti-Cas9 Western blotting was performed on whole-cell extracts from stable pools of A549...blasticidin selection. Anti-Cas9 Western blotting was performed on whole-cell extracts from the initial stable...should appear less green. Note that there won’t be uniform editing across all the cells in the population,...techniques and how quick and easy it is to share information and regents, it’s exciting to think that no matter...

...predicted unstructured loops are best since these tags form beta-hairpins (Madani et al., 2009). Donor and acceptors...

...fill out our form to let us know what you would like to see available in viral prep format. Please note...plasmid ( Addgene #157970 ). Viral preparations are formulated in one of the following three buffers. All buffers...was developed by Shannon Boye’s lab. Plasmid Information The plasmid below is available in each of the...Please click on the plasmid name for ordering information. ID Name Promoter Description Category PI 157970...(CBA) GFP Control Sergei Zolotukhin Citation Information AAV2(Y444F) When using the AAV2(Y444F) serotype...

...CRISPR system consisting of a catalytically inactive form of Cas9 (dCas9) plus guide RNA (gRNA) for biochemical...biochemical purification (Fig. 2). For additional information and protocols, check our references for the plasmids...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...

...fill out our form to let us know what you would like to see available in viral prep format. Please note...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...researcher safety. Read our Lentiviral Guide for more information about: Lentiviral components 2nd versus 3rd generation...transgene or shRNA. See article for detailed information about cloning & additional plasmids. Aebischer...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...expression for Gateway cloning; selection cassette in format: PGK-rtTA-2A-puro; see article for tagged insert...transgene or shRNA; see article for detailed information about cloning and additional plasmids tetR-KRAB...expression of a miR-shRNA; selection cassette in format: rtTA3+IRES+Neo; see article for additional selection...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...described below. Read our CRISPR 101 post for more information about HDR/repair template design. How to use ...

... EB-1B requires that you provide two out of six forms of documentary evidence: Evidence of receipt of... of this post are intended to convey general information only and not to provide legal advice or opinions...particular circumstance or fact situation.  The information presented may not reflect the most current legal...No action should be taken in reliance on the information contained in this post and we disclaim all liability...intentions directing federal agencies to propose reforms to the H-1B visa system. Large firms and firms ...to most postdocs. Commercial successes in the performing arts- not relevant at all. Please do not include...you are a classically trained ballerina who has performed at Lincoln Center. Your case should only focus...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...myristoylation signal For more information about tags, including amino acid sequences, see...delivery. Visit our viral vector page for more information. Below are some common delivery methods: Delivery... host genome. View our AAV page for more information. pAdTrack-CMV - Shuttle vector...

...Protein phosphorylation is one of the most abundant forms of posttranslational modifications in cells and ... is anticipated to be useful for laboratories performing phosphoproteomic experiments in need of reference...

... including (1) a CRISPR RNA, (2) four proteins forming the QCascade DNA-targeting module with the crRNA... of bacteria including in pooled, genome-wide, formats. Interested in knowing more? Check out our Mobile... WA (2013) Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression...

...long fluorescence lifetime, and a stable monomeric form as determined by the organized smooth endoplasmic... 9 amino acid N-terminal variation in the Klf4 isoform (KLF4s or KLF4L). Polycistronic cassettes that ...Polycistronic cassettes that contained the KLF4L isoform had more robust initiation of reprogramming (evaluated...All three mScarlet variants have shown great performance in cellular functional imaging and in protein... These Lmo proteins were identified using a bioinformatics approach examining genomes possessing CRISPR...

...protein and in vitro transcribed gRNA are combined to form a Cas9-gRNA complex, which is delivered to cells... be used to examine off-target effects. More information on each of these techniques can be found in our...

...fill out our form to let us know what you would like to see available in viral prep format. Please note...Plasmids and Optogenetics Guide page for more information about this field. Use the table and filters below...

...fill out our form to let us know what you would like to see available in viral prep format. Please note... vivo testing of these viral preparations was performed by the Karpova lab. Results for our quality control...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this... AAV production. Read our AAV Guide for more information about: AAV components Common uses of AAV Viral...plasmids encode the AAV genome (i.e., the genetic information that will be contained in the virion) and/or ...

...fill out our form to let us know what you would like to see available in viral prep format. Please note...Addgene's blog on Systemic Capsids . To review performance data of each of these serotypes, please refer...window) ) to cross the blood brain barrier. For information on whether the PHP.eB serotype will work in your...pAAV-CAG-GFP CAG GFP Control Boyden Citation Information When using the Caltech serotypes in future publications...

...ll express your gRNA and Cas9 protein, which will form a ribonucleoprotein complex through interactions...effector, composed of several separate Cas enzymes that form a complex called Cascade (see Cas3 for more details...with Cas3 Rather than initiating DNA cleavage to form a break, Cas3 initiates single-strand DNA degradation...dCas9 Nuclease dead Cas9, an enzymatically inactive form of Cas9; can bind, but cannot cleave DNA DSB D ouble...Cas9 undergoes a second conformational change upon target binding. In this formation, its nuclease domains...detection platform. Based on this non-specific cleavage, a Cas13a-based molecular detection platform, termed...positively-charged grooves on Cas9. gRNA binding induces a conformational change in Cas9, shifting it into an active,...