...things like DNA synthesis, Golden Gate cloning, and CRISPR. The yeast synthetic genome project and constructing...

...Mutations in Your Plasmids Learn How You Can Use CRISPR Pooled Libraries to Generate Mutant Populations...Addgene.org Find Plasmids from the Whitehead Lab Use CRISPR Pooled Libraries to Generate Mutants ...

... may recall our first 20th anniversary story on CRISPR, where we dove fairly deeply into the different...Practical Considerations for Using Pooled Lentiviral CRISPR Libraries. Current Protocols in Molecular Biology...

...With the meteoric rise of CRISPR technology, the ability to direct enzymes – from nucleases to transcription...

...Has Been Used in Cancer Lineage Tracing Read How CRISPR Can Be Used in Genome-Wide Screening Browse 28 ...

...Needed to Navigate a Scientific Enterprise in Flux CRISPR Kinome Libraries Available: Pooled and Individual...

... of the topics they've recently covered inlcude CRISPR, mosquitoes, allergies, & more! We briefly answer...

...BMC Biotechnol. 2006 Dec 7;6:46. John McCafferty CRISPR system to rapidly engineer the constant immunoglobulin...diversification of hybridoma-produced antibodies by CRISPR/HDR genomic engineering. Sci Adv. 2019 Aug 28;5...

...PMC2258639. Resources on the Addgene Blog Learn How CRISPR Can Be Combined with Phage for Antimicrobial Purposes...Systems to Probe Protein-Protein Interactions Use CRISPR For Genome-Wide Screens Resources on Addgene.org...

...RNAi to knock-down expression of your target or CRISPR/Cas9 to create mutations that essentially eliminate...the gene for your protein has been mutated using CRISPR/Cas9. You check the mutation and it causes a large...

...Retinal Ganglion Cells AAV/CRISPR Mouse Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms ...mitosis. Nat Commun. 2016 Mar 7;7:10869. Hu CRISPRa Human CRISPR activator system for reprogramming human...enogenous genes Human pluripotent reprogramming with CRISPR activators. Nat Commun. 2018 Jul 6;9(1):2643. Otonkoski... Brennand iPSCs Cortical or Lower Motor Neurons CRISPR/TALEN Human Transcription Factor-Mediated Differentiation...

...fluorescence, SunTag can also be used to improve CRISPR-based activation of target genes, but we’ll save...

...via Addgene. Given the increasing popularity of CRISPR in the plant community, this has saved us hours...

...the recent release of the Spanish version of our CRISPR 101 eBook and the upcoming release of the second...

...Kinase ORF collection Fluorescent Protein Tools CRISPR Plasmids This resource is a collaboration between...

...generated a number of Shared Research Tools , such as CRISPR genome editing tools deposited with Addgene. Browse...

...Engineering Technologies TALEN Engineering Reagents CRISPR Plasmids...

...therapy. SB is a valuable tool and an alternative to CRISPRs for genome engineering whose use is only likely...

..., they eliminate the expression of the gene (by CRISPR knockout, shRNA depletion, etc.) and ask what the...

...on Addgene.org Find simple lab protocols Browse CRISPR plasmids for isolating a genomic locus Browse FRET...