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  2. TALEN Guide

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    Collection
    ...TALEN Technologies You may also like... TALEN Kits CRISPR Plasmids Zinc Finger Consortium Originally published...Nucleic Acids Research article that came out in the spring of 2011, the groups describe a set of customized...

  4. Lentivirus Plasmids

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    Collection
    ...lentiviral plasmids to express your CRISPR gRNA? Please see our CRISPR Empty gRNA plasmids table and search...

  5. New Podcast Segment: Hot Plasmids

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    Blog Post
    ...plasmids covered in this segment Hot Plasmid 1: CRISPR X from Michael Bassik's Lab - A new tool for making...localized point mutations. Listen to Our Segment on CRISPR X Hot Plasmid 2: Magneto 2.0 form Ali Guler's Lab...

  6. MassBioEd Career Exploration Day at Addgene

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    Blog Post
    ...Architect Pam McA' Nulty (check out our blog post on CRISPR software to see the importance of programming for...Scientist Joel McDade’s presentation on the emerging CRISPR genome editing technology was a rousing success...

  7. Microbes: Look, Listen, and Tell

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    Blog Post
    ...Resources on the Addgene Blog Read Our Post on CRISPR Methods for Bacterial Genome Engineering Learn ...Addgene.org Check out Our Microbiology Resource Page CRISPR plasmids designed for use in bacteria Deposit Your...

  8. Three Tips to Organize Your Lab Notebooks in the New Year

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    Blog Post
    ...you’re looking for. Tip 3: Share protocols and spreadsheets with lab members Now that you’ve organized your... may also want to consider moving your paper spreadsheets for reagent storage locations, such as the list...

  9. Supporting Rare Disease Research with BeHeard 2017

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    Blog Post
    ...proteins to monitor the localization of these genes, CRISPR to disrupt their function, and C. elegans overexpression...peritoneal mesothelioma tumorigenesis. They plan to use CRISPR and lentiviral plasmids from Addgene to knockout...

  12. Plasmids 101: Gene, enhancer, and promoter trapping

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    Blog Post
    ..., they eliminate the expression of the gene (by CRISPR knockout, shRNA depletion, etc.) and ask what the...e67667. DOI: https://doi.org/10.7554/eLife.67667 Springer, S. P. Gene Traps. Plant Cell. (2000) 12(7): 1007...

  15. Cultivating Community Science at BosLab

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    Blog Post
    ..., our journal club discussion on Kevin Esvelt’s CRISPR/Cas9 gene drive paper was very popular. People ...Blog Experimenting in an Open Source Lab: from CRISPR to Cats Stem Cell Models for Disease & Open Science...

  16. Plasmids 101: NGS Quality Control for Pooled Libraries

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    Blog Post
    ...analysis script should return the same output: a spreadsheet of read counts. You can use these counts to confirm...Sequencing to Validate AAV Preps Learn More about Pooled CRISPR Libraries Resources on Addgene.org Browse Pooled...

  19. Synthetic Photobiology: Optogenetics for E. coli

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    Blog Post
    ... of this far-red/red light-responsive system is spread across three plasmids, with the response regulator...limited by a few factors. These bulky systems were spread over a number of plasmids, with one RR (ompR) encoded...

  20. Luminescent Imaging with Nano-lanterns

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    Blog Post
    ...chemiluminescent proteins for imaging, but it has precluded widespread adoption of the technology. In 2012, Saito et...genes for endogenous protein fusions created using CRISPR/Cas9. eNLs offer high luminescent signal for imaging...
Showing: 341 - 360 of 554 results