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  1. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...viral services. After a month or so of meetings, I began to notice something: even though our scientists ...sample,” did the word convey the same concept to Morgan, our senior engineer? The answer to these questions...

  3. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...guest blogger and Future of Research Symposium organizer, David T. Riglar PhD. Here, Dr. Riglar discusses...experience from the perspective of both grassroots organisations that represent postdocs and institutes that ...

  5. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...Green. Zika background First identified in 1947 in Uganda, the Zika virus had received little attention and...Guillain-Barré syndrome. In response, the World Health Organization has declared a global health emergency and called...

  7. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...available. Table 1: Fragmid destination vectors Organism Delivery Type Destination Vector Mammalian...select the features of your vector, which are: Organism (Mammalian or drosophila) Delivery type (lentivirus...

  10. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...Mice are a common model organism used to understand mammalian traits and genetically engineered mouse...tamoxifen-inducible Cre (Cre-ER) systems, Cre is fused to the ligand binding domain of estrogen receptor alpha (Esr1...

  11. Lab Automation at Addgene

    Type
    Blog Post
    ...mail due to laws concerning genetically modified organisms. Since Addgene is very proud to help scientists...labels than your hand and help keep things more organized. Cap tubes with a micronic cap sealer. This is...

  12. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...visualization of proteins of interest. A fluorescent Halo ligand can be added to media, buffer, etc., which will...target protein and an E3 ligase through interacting ligands. After an E3 ligase facilitates ubiquitination ...

  13. Transferable Skills: Negotiation

    Type
    Blog Post
    ...with your colleagues within and outside of your organization. In my non-academic roles, some examples of ...development analyst, I negotiate with global organizations that partner with Addgene. What are the most...

  18. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...specimens and for in vivo imaging of transparent organisms. Advances in tissue clearing Similar to light ...3DISCO (3-dimensional imaging of solvent cleared organs) method of imaging rodent brains and spinal cords...

  20. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...fluorescent when ectopically expressed in most organisms. Furthermore, fusing FPs to another protein usually...Additionally, many other FPs have been found in other organisms. Blue 424 - 467 nm Cyan 474 - 492 nm Green...
Showing: 341 - 360 of 601 results