Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 610 results for: GAN

Showing: 341 - 360 of 610 results

  4. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...specimens and for in vivo imaging of transparent organisms. Advances in tissue clearing Similar to light ...3DISCO (3-dimensional imaging of solvent cleared organs) method of imaging rodent brains and spinal cords...

  6. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...fluorescent when ectopically expressed in most organisms. Furthermore, fusing FPs to another protein usually...Additionally, many other FPs have been found in other organisms. Blue 424 - 467 nm Cyan 474 - 492 nm Green...

  7. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...technique utilizes Cas9 proteins harboring receptor ligands (C in the figure above), which result in the internalization...allowing for ligation to pyridyl disulfide-activated ligands. Due to the concerns of off-target mutations and...

  8. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...figure. Some follow the same figure by figure organization with a different person responsible for each...journal clubs should be well planned and carefully organized. Most importantly, they should be regularly assessed...

  10. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ..., which connect the heart, lung, gut, and other organs to the CNS, are also an important target for gene... Percent of Neurons Transduced   Dorsal Root Ganglion (DRG) AAV.9 (parental) 46 +/- 0.7% AAV.PHP.S...

  11. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...these vectors for gene therapy. In 2003, VIRxSYS began the first clinical trial with a lentiviral vector... vector. Naldini L, Blömer U, Gallay P, Ory D, Mulligan R, Gage FH, Verma IM, Trono D. Science. 1996 Apr...

  12. CRISPR Antimicrobials

    Type
    Blog Post
    ...their suppliers, and public health and medical organizations are working to help educate the public about...chance of an opportunistic infection like for organisms like C. difficile. In 2014, Citorik et al. and...

  13. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...directly from A. Victoria and expressed in any organism while still maintaining fluorescence. The protein...soon as its sequence was elucidated, scientists began engineering new versions of GFP through mutagenesis...

  14. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...plant tissue cultures.   C. roseus is not a model organism such as Arabidopsis or tobacco. Studying gene ...transgenic plants is a common problem in non-model organisms. Therefore, transient methods are usually the ...

  15. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...and stably maintain the genes they carry among organisms belonging to many different phylogenetic groups...bacteria and express proteins within different host organisms. pBBR1-based plasmids and plasmids in incompatibility...

  16. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...entirely. Auxotrophic selection relies on the host organism being unable to synthesize a nutrient required... to design your vector to be functional in the organism your experiment takes place in. In many cases,...

  17. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...specific to that country. ‌For example, when we began offering temperature-sensitive materials, we noticed...materials and learn about them. As early as 2007, we began grouping similar tools together in what would eventually...

  18. Hot Plasmids - November 2023

    Type
    Blog Post
    ...proximity labeling experiments in cells, tissues, and organisms. Lee, S.Y., Cheah, J.S., et al. (2023). Engineered...hottest antibodies in Addgene’s collection since we began distribution and has been verified to perform well...

  19. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ... salaries (which have been made by multiple organizations across academia). Increased postdoc wages, overtime... salaries as their website implies. These 5 organizations, according to the NSF data, account for 18% ...

  20. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...region (approximately 500bp is best) and select the organism. It will then tell you the available gRNA targets... much more from our favourite mammalian model organisms in the near future.  Many thanks to our guest...
Showing: 341 - 360 of 610 results