Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection
Type
Blog Post
...instance, GFP may occlude catalytic sites or obstruct binding sites or motifs that are necessary for post-translational...avidity because it doubles the number of binding sites per unit.
There are several ways to measure stickiness...Where should the fluorescent protein be inserted? Sites that are less likely to disrupt the structure of...this is not conclusive evidence that the fusion operates in the same way as the native protein. Although...PMC4927352.
Janetopoulos, Chris, Tian Jin, and Peter Devreotes. "Receptor-mediated activation of heterotrimeric...