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  1. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    ... confident that their teammates and project collaborators have completed their work thoroughly. It’s kind...Veckerelli is the Administrative and Operations Coordinator at Addgene. In addition to her operational responsibilities...

  3. Hot Plasmids - November 2023

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    Blog Post
    ...example, neurons treated with a phorbol ester activator gave fluorescent signal in the ticker tape from...transmission, and plasticity and is a popular marker of excitatory synapses in assays like IHC. In fact, Anti-PSD...

  5. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...more feasible, the lab of Alessio Ciulli and collaborators developed CRBNmidi (Kroupova et al., 2024). ...Bentley Time to get flexible! Alex Dunn’s lab and collaborators have deposited a new mechanosensor called PILATeS...

  6. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...available in the repository. GCaMP, a calcium indicator (biosensor, by Addgene’s categorization) was first...Targeted Neuronal Manipulation. Frontiers in Neuroanatomy, 13. https://www.frontiersin.org/articles/10.3389...

  7. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...carefully regulated by a mix of positive and negative regulators. The genome of E. coli is about 4.6 million ...trading out ADP for ATP to re-activate DnaA. These regulatory mechanisms allow E. coli to control chromosomal...

  8. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ... region is transferred into the plant. In the laboratory we can utilize this natural process by replacing...and a vacuum is applied with a vacuum bell or desiccator. Successful infiltration can often be seen by...

  9. Antibiotic Resistance: An Old Solution but a New Problem

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    Blog Post
    ...resistant infection or not, because scientists in the laboratory will need to isolate and grow whatever bacteria...method that is frequently used in the clinical laboratory. What can we do to stop the emergence of antibiotic...

  10. Using Video to Share Your Science: We Share Science

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    Blog Post
    ...studies on reducing antibiotic prescriptions for respiratory tract infections. The video abstracts provide...  “Singing” Fish Rely on Circadian Rhythm and Melatonin for the Timing of Nocturnal Courtship Vocalization...

  11. Neuronal labeling with Spaghetti Monster

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    Blog Post
    ...famous ones are influenza hemagglutinin (HA), myelocytomatosis viral oncogene (myc), simian virus 5-derived...usually provided by red fluorescent proteins such as tdTomato or RFP or blue fluorescent proteins like Cyan....

  12. Fluorescent Proteins 101: Aptamer Fluorophores

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    Blog Post
    ...fluorophore protein. Years later, Samie Jaffrey’s laboratory took advantage of their deep knowledge of the...Farzin. RNA Nanotechnology and Therapeutics. Boca Raton: CRC Press, 2014. 5. Hayat, Akhtar and Marty, Jean...

  13. Plasmids 101: Broad Host Range Plasmids

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    Blog Post
    ...different bacterial hosts (Doran et al. 1998). Regulatory elements within the ori also control copy number...own replication machinery such as Rep or other initiator proteins, they do not require host proteins for...

  15. Fluorescent Protein Guide

    Type
    Collection
    ...signals, cells, or groups of cells with either actuators or sensors. Chemogenetics Plasmids | Guide Use...

  16. AAV Viral Preps

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    Collection
    ...Monster, Brainbow Molecular Tools Tetracycline Transactivators, Affinity Purification, Cell Ablation Monosynaptic...

  17. CRISPR Plasmids and Resources

    Type
    Collection
    ...Transcriptional Regulation Activate dCas9 fused to an activator peptide activates gene expression. Interfere dCas9...

  18. FlyCRISPR

    Type
    Collection
    ...designed for in vivo transcrition controlled by the regulatory sequences of an RNA polymerase III transcribed...

  19. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...It helps to come from the lab of a well-known investigator. If you only have middle author papers or no...Einstein College of Medicine in the Department of Anatomy and Structural Biology, and now serves as the Director...
Showing: 321 - 340 of 641 results