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  1. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...better plan your transductions and use a range of reasonable MOIs. 2. Create a monoclonal Cas9-expression ... gRNA plasmids on our website. There are a few reasons to target different regions of the gene with multiple...testament to how far genome engineering has come. For comparison, in 2007 it took about 3 months to knockout a...

  2. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    ...commonly used for recombineering can be found in Thomason et al and Sharan et al (a few strains can also...ecosalplus. PubMed PMID: 27223821. 4. Sharan SK, Thomason LC, Kuznetsov SG, Court DL. Recombineering: A ...19180090. PubMed Central PMCID: PMC2790811. 5. Thomason, L. C., Sawitzke, J. A., Li, X., Costantino, N...

  3. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...that contains sequence complementary to the dsRNA (Wilson and Doudna, 2013). RNAi consists of two non-exclusive..., Deans RM, Li A, Bassik MC (2016) Systematic comparison of CRISPR/Cas9 and RNAi screens for essential...:dev186452 . https://doi.org/10.1242/dev.186452 Wilson RC, Doudna JA (2013) Molecular Mechanisms of RNA...

  4. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...difficult to trace. These include the laboratory personnel, the serum, the cell culture media, water baths...your cell cultures because you are not the first person to face this kind of problem. Educate others when...Sung H, Kang SH, Bae YJ, Hong JT, Chung YB, Lee CK, Song S (2006) PCR-based detection of mycoplasma species...

  5. Typing CRISPR Systems

    Type
    Blog Post
    ...P. C., Shmakov, S. A., Silva, C., Tolstoy, I., Johnson, D. B., Koonin, E. V., & Quatrini, R. (2021). Evolution...https://doi.org/10.1093/nar/gkz1197 Samai, P., Pyenson, N., Jiang, W., Goldberg, G. W., Hatoum-Aslan, ... Hallmark, T., Keiser, D., Bondy-Denomy, J., & Jackson, R. N. (2021). Positioning diverse Type IV structures...

  6. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ... is FRET and what's it good for? Fluorescence resonance energy transfer (FRET) is a biophysical interaction... excited, it can transfer energy directly via resonance to the second fluorophore (the acceptor) if the...PMID: 17512008. 4. Fessenden, James D. "Förster resonance energy transfer measurements of ryanodine receptor...

  7. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...heteroduplex transitions back to DNA duplex, and it’s the reason guide sequences have a maximum length of 20 bases...Sternberg et al., 2015).     Figure 8: Comparison of Cas9:RNA crystal structure (left, PDB ID 4ZT0..., Dangerfield, T. L., Jung, K., McCool, R. S., Johnson, K. A., & Taylor, D. W. (2022). Structural basis...

  9. CRISPR 101 eBook...en español

    Type
    Blog Post
    ...repositorio. Este increíble regalo permitirá que más personas accedan a recursos útiles para sus experimentos... Rachel Leeson...

  10. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    ... research, for decades. Its short life cycle, reasonably close genetic homology to humans, and small size... Rachel Leeson...

  12. 2021 Deck the Lab Winners!

    Type
    Blog Post
    ...today✂️❄️🦠thanks to the awesome templates by @CVRHutchinson @CVRinfo. Check them out: https://t.co/uDEE9PsspD...  Rachel Leeson...

  13. Congrats to the 2019 Deck The Lab Winners

    Type
    Blog Post
    ... of holiday spirit still linger (despite the unseasonably warm weather we had last weekend). We’ve spent...DeckTheLab. Getting in the spirit for the holiday season. Santa frog is coming to town. @Addgene @UMich_...

  15. DIY DNA Ladders from Penn State University

    Type
    Blog Post
    ...Addgene. A team of undergraduate students led by Dr. Song Tan at Penn State developed the plasmids, pPSU1 ...1000 Lanes Cost $200-$500  <$10 $20-30 Cost Comparison: While convenient, commercial ladders cost between...

  16. Summer Fun at Addgene!

    Type
    Blog Post
    ...Here at Addgene, we’re always inspired by the new seasons, so we've made some big summer plans this year!...                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      Rachel Leeson...

  17. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    ... to #DeckTheLab with virus snowflakes from @CVRHutchinson #SciArt #STEAM #WinterWonderlab @Addgene Make...We hope everyone has a safe and festive Holiday season. 🙌 🥂 🎄 🎉 😷 Remember wear a mask,↔️social distance...

  18. Editor's Choice, July 2016

    Type
    Blog Post
    ...easier to generate mouse models.   Cpf1 Update: Comparison to Cas9 and NgAgo As you might expect given the...Generating Mouse Models Using CRISPR/Cas9 Cpf1 Update: Comparison to Cas9 and NgAgo Special Delivery: Fluorophore...

  20. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    ...introduction to plasmids with Director of Biology Lianna Swanson was very popular, with the students excited to ...different career options in science, with Abigail Anderson commenting that “There is not just a scientist...
Showing: 321 - 340 of 718 results