...approach – co-CRISPR from Craig Mello’s lab – used inactivation of the unc-22 gene as their selection marker...Drosophila S2 cells (Böttcher et al., 2014), inactivation of non-homologous end-joining results in a further...changes 5’ to the PAM, sequences corresponding to the same strand as the PAM produced a higher editing efficiency...elegansGenome with Lethal Mutation Co-Conversion and Inactivation of NHEJ Repair. Genetics 199:363–377 . https...

...This can differ between fluorescent proteins in the same spectral class (Cranfill et al, 2016). This non-...described by a mono-exponential decay (analogous to radioactive decay). Consequently, the intensity decay is ...comparison of several fluorescent proteins of the same spectral class on the microscopy system and in the...

...classic approach for studying protein turnover is radioactive pulse-chase experiments. However, these experiments...require radiolabeled amino acids. The use of radioactive materials is highly regulated, requires designated... suspected to interact, are added to a cellular sample. Both antibodies have a short antibody specific...

...your poster. While a poster generally contains the same sections as a primary research article, it’s important...can be accomplished with an exciting title and attractive figures. Next, the poster should be legible from...four column style, and in all cases the idea is the same; the reader will navigate the poster from column...

...paying bills and having a life outside my job. The same can be said about teamwork, communication, writing... every piece of work you touch, and be a more attractive candidate for that ideal job. How can you identify...process. You stepped up and got your team on the same page. This came naturally to you. How is this new...

...under the control of an inducer, i.e. tet-off transactivator (tTA), is injected at a high dose to maximize...An AAV expressing the inducer (i.e. tet-off transactivator) is co-injected. The dose of this AAV can be... Central PMCID: 5088052. 3. Lentz TB, Gray SJ, Samulski RJ. Viral Vectors for Gene Delivery to the Central...

...fluorophore-labeled antibodies with very specific reactivities making them more useful for multicolor imaging...super-resolution STORM imaging, and electron microscopy. Sample preparation for these techniques sometimes makes...smFPs retain their ability to be detected after sample preparation better than standard proteins tags ...

...Additional uses The cover letter is a great place to proactively address questions about your application. These...Structure of a cover letter Cover letters follow the same format regardless of whether you’re in academia,...requirements, and address any unusual circumstances or proactively answer questions the hiring manager may have....

...Green Lab website, but if you don’t see one, be an ACTivist and encourage companies to obtain these labels...cytometers. These fluidic machines handle biological samples and are cleaned between each use. You wouldn’t ...

... (e.g., apple, papaya, and tomato) and the photoactivatable fluors (e.g. Phamret and Dendra). It’s just...them,” Baird said. “They are all designed in the same vector backbone with very similar restriction enzymes...

...saving freezer space).  The procedure becomes less attractive when noting that one step involves boiling the...less stop buffer and miniscule amounts of plasmid sample.  How satisfying?  With practice, I got good at...

...the latest fluorescent proteins, I maintain an interactive graph and table of the best fluorescent proteins...be imaged together, you will need to consider the same factors as when choosing an individual fluorescent...

...modification, or add in fluorescent or chemically reactive side chains, all of which can help determine structure...naturally, rarely are the optimization parameters the same for all three components, meaning that the final...

...community is loving it. Almost 10,000 viral vector samples were distributed in 2018 and we are poised for ...Work with a positive, supportive culture, we are attractive to the local employee pool and we get a lot of...

...both lentiviruses and gamma-retroviruses use the same gene products for packaging, however, the isoforms...(Plasmid #44246) Expression of a catalytically inactive (dead), human codon-optimized Cas9 under the control...

...that available and accessible weren’t always the same thing, shaped both Addgene’s founding and our growth...design in our digital content. We have implemented proactive measures to find potential barriers that may not...

...their disciplines. Included in the article is an interactive chart where you can see the yearly interdisciplinarity...amount of interdisciplinary research while at the same time increasing interdisciplinary citations in their...

...require modifying multiple genes simultaneously in the same cell, are more appropriate for a CRISPR-based approach... mechanisms and consequences of cohesin gene inactivation in human cancer.     References 1. Russell, ...

...Saturday Hack Day projects sought to make an interactive game that highlights just this point. As part...underrepresented groups under their mentorship with the same critical eye they place on others within their labs...

...three loxP variants, but the principles remain the same. The first fluorescent protein in the construct,...proteins and confirmed that there was no cross-reactivity, opening the door for immunostaining analysis...