...Mentee. A Mentor will be more effective if the Mentee has done the self-reflection necessary to identify areas... you have nothing to give, think again. Everyone has knowledge to impart. An undergraduate can mentor ...relationship, but if you don’t take this past the chatting stage you will rarely make any change in your skill sets... the helpful accountability that is one major advantage of working with a mentor.The responsibility of...graduate student – you get the idea. There are many advantages to being a Mentor: Learn other perspectives ...

...   Fig. 1: SpCas9 has sparse PAM coverage in the human genome. SpRY Cas9 has PAM sites covering the... a PAM with no sequence constraints, or at least has looser or different requirements. Luckily, there ...alternative PAM requirements (Zhao et. al, 2023). xCas9 has been shown to be less effective at NGH PAMs, though... PAM requirement, and how these proteins are advantageous in today’s genome editing landscape. The need...

..., that informs us that this antibody has specific binding and has a strong signal. We call the unique ...strong signal has a shorter exposure time than the negative control, and a weak signal has a longer exposure...rAbs are a relatively new technology that takes advantage of DNA sequencing to turn mAb clones into expression...

...easily assemble multiple DNA fragments. This system has been used to construct plasmids for use in model ...Cyanobacteria, a phylum of photosynthetic bacteria, has increasingly been recognized for its biotechnological... encoded voltage indicator Article contributed by Shreya Vedantam Genetically encoded voltage indicators...GEVIs) are proteins that detect and output the voltage changes in neural activity when paired with appropriate...appropriate fluorescent optics. Existing red fluorescent voltage indicators, however, fall short compared to GFP-based...containing a red-shifted Ace-mScarlet, a fusion of a voltage-sensitive inhibitory rhodopsin (Ace2N) with the...amino acid linker between them. The Ace-mScarlet voltage indicator works like many FRET-opsin GEVIs. With...

...industry just wasn't for me, and as has been highlighted recently it has become more difficult to attain ...interesting and luckily genetics is a sector which has been rapidly growing. These factors left me optimistic...because it is a small and dynamic company and so it has the flexibility of a ‘start-up’. I was surprised ...schedule.  I really love my highly varied role, it has allowed me to travel and to share my passion for ...everyone. My career path may seem quite unusual but it has allowed me to try out working in many different sectors... still feel now, that it is important to take advantage of the fact that science is a global language ...

...host cells immediately prior to their transduction has been shown to increase AAV transduction efficiency... such cationic reagent that is commonly used and has been shown to increase transduction efficiency by...single-use aliquots. Never use a polybrene solution that has been thawed more than once. Fibronectin is another...polybrene (e.g., hematopoietic or primary cells), and has been shown to increase transduction efficiency by...packaging cells after 1–3 days of selection. A lower percentage of surviving cells may be the result of plasmid...

...Griesbeck adds that this color change has a particular advantage for observing events taking place inside...Griesbeck of the Max Planck Institute for Neurobiology has been working on genetically encoded indicators of...Maarten Merkx of Technische Universiteit Eindhoven has similar interest in FRET-based sensor proteins, particularly...

... techniques is a speculation of yesteryear which has been realized recently. Science Fiction (SciFi) writers...even before the discovery of genes themselves.  How has scientific progress in genetic editing impacted the...technology on various non-scientific platforms, CRISPR has also transcended into the world of SciFi. This is...and most important genres of literary fiction. It has always served as a vision of the future and to make...and agriculture, its portrayal in popular fiction has been largely negative. But these negative depictions....1126/science.1258096 Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR (2018) Publisher... movie version (2016) stars Tom Hanks as the protagonist (Langdon). The plot involves Langdon chasing ...

...For example, the Levin lab from Tufts University has used Arch (green-light activated proton pump) and...Allen Discovery Center. The future of this field has a high potential for the advancement of research ...based on the fusion of the voltage sensing domain of a Ciona intestinalis voltage sensor and a mutated version... reporting and genetically encoded fluorescent voltage indicators (GEVI) are ideal tools to track bioelectric... 2018) available at Addgene: MerMAID2 - A metagenomically discovered marine anion-conducting channelrhodopsin...channelrhodopsin used as a sensitive fluorescent voltage indicator with near-complete desensitization in continuous...Endogenous bioelectric signaling networks: exploiting voltage gradients for control of growth and form." Annual...

...top of the resolving gel. A stacking gel typically has a different ionic strength and lower pH and acrylamide...Choosing your primary antibody Once the membrane has been blocked, it is stained with a primary antibody...the vendor’s website and choose an antibody that has been validated for immunoblotting. Different immunoassays..., cytoplasm and nucleus.  We hope that this blog has provided you with a good overview of the western ... of the gel. For small proteins, use a higher percentage of acrylamide to increase migration resistance...improve separation. For large proteins, reduce the percentage of acrylamide. To further improve resolution,...

...Not the case, as I’ve discovered.) As someone who has now seen both sides of the postdoc experience, I’... lab is closely involved with clinical trials or has ties to industry, the drug discovery process can ...Genentech, will not. The point is that each company has a unique approach, and some might suit your needs...currently a Postdoctoral Scientist at AstraZeneca. She has a background in cell and developmental biology and...number of steps, decisions and people involved is staggering. The process is complicated. If you’re interested...

... ddPCR.) qPCR Quantitative/real-time PCR (qPCR) has been used for copy number assays for years. qPCR ...and quencher to quantify specific amplicons.  Sybr has high background noise, due to the large amount of...AT-rich DNA and the length of the amplicon and it has a higher rate of false positives (Colborn, 2008; ... used to accurately call copy numbers, though it has been shown to identify trends correctly (Muhamad,...Muhamad, 2011;  Providenti, 2006). Its biggest advantage over Taqman is price point, which should be carefully...

...community has access to all of the latest resources? In response to this challenge, Addgene has launched... why recent work in the field of cancer research has focused on picking apart the complex cellular pathways...at Vanderbilt University in Nashville, Tennessee has already demonstrated that a number of novel small...ensues, traditional chemotherapies have taken advantage of the universally-dividing behavior of cancer...

...attributes (which turned into www.fpvis.org).  That page has been very useful over the years, and when researchers...system that allows users to see when a given page has unmoderated changes and to view previous versions... online collections of FP spectra. Every protein has a page with all the information in the database relating...of FPbase FPbase is still very much in the early stages of development, and there is plenty of room for...

...birth several weeks previously. While this claim has yet to be independently verified, the watching world...Kingdom’s Nuffield Council. While the Nuffield Council has recently published a report on the social and ethical...considerations, in making sure that the research has been adequately proven to be reasonably safe and ..., in vitro research, animal models, and several stages of human clinical trials. This model also demonstrates...

...easy. Most of our equipment has been donated by local companies and BosLab has grown into an organization... biologist with a Ph.D. from UNC-Chapel Hill and has extensive experience targeting RNA with small molecules... entrepreneurs using our space to perform early-stage experiments. For example, Dave Grawoig is using ...

...booklet. What has been the most surprising thing about becoming a PI? Tom Ellis has a one word answer...prepared me for what a PI without a personal assistant has to deal with. My inbox currently stands at about ... build a strong, collaborative team. And take advantage of the many resources available from your institution...

...Find Empty Backbones at Addgene Each approach has advantages and drawbacks, which are summarized in the ...for the presence of an insert. Traditionally this has been done with restriction enzyme digest; however...insert-specific primers but also tell you whether the insert has the correct directionality. Verifying the insert ...and are more likely to work in a PCR reaction that has bacterial debris. Use a positive control. A good...

... population level, but each individual cell only has a maximum of one gene knocked out. In this genetically...containing up to around 200,000 different gRNAs. It has been suggested that researchers should use ~400X ...than gRNAs in a screen, to ensure that each gRNA has a good chance to contribute to the screen. In the... If you are lucky and find that a druggable gene has a central role in your system, you could majorly ...guest blogger Fredrik Wermeling! Fredrik Wermeling has a research group at the Centrum for Molecular Medicine...Medicine, Solna, Karolinska Institutet, in Sweden. He has a particular interest in using molecular tools to... differentially expressed genes can be the next stage in making sense of such data. These targeted screens...

...restriction endonucleases. A given prokaryote typically has genes encoding one or a few restriction modification.... Using the same example plasmid as above (which has the XbaI site blocked by methylation), the figure...newly synthesized product from a site-directed mutagenesis experiment? The answer to both questions is the...to methylation if the enzyme's recognition site (TCTAGA) is preceded by GA or followed by TC. As shown ...cleave DNA. DpnI is often used for site directed mutagenesis. During this process, incorporation of a desired...Troubleshoot Your Sequencing Results Use REPLACR Mutagenesis to Mutate Your Plasmids Resources on Addgene.org...