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  1. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...tools by increasing gRNA stability. First, they began with the Broccoli aptamer system to visualize the... will recruit the HaloTag with red fluorescent ligands to the loci of interest. Dual color detection In...

  4. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ...develop to reach their full potential in the organization. This will result in more engaged, successful...Series Learn How Volunteering at a Professional Organization Can Help You Develop Leadership Skils Set Concrete...

  5. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...for easy genome engineering in species like C. elegans and Drosophila. Light inducible promoters Light...transcription machinery differs between cell types or organisms, promoters are similarly variable. Bacterial promoters...

  6. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...Neuroscience covers many subfields When I first began looking into the details of SfN17, I expected that...addition, there was an entire story-telling session organized by Story Collider and which featured a talk about...

  8. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ... environment, all while reciting their favorite slogan, “look how cool the brain is!" Using retrograde...account since activation with chemogenetic specific ligands activates both neurons at the injection site and...

  9. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ...mutagenizing a population (typically cells or small organisms) and then assaying for a functional or phenotypical... events. To put it another way, only cells or organisms with the desired phenotype will survive and be...

  10. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...systemically transduce and then retransduce a model organism such as mice, e.g. first at 2 months, then at ...field has settled on ‘best practices’ for model organisms.    Q11: How consistent is expression is when ...

  13. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...processing machinery present in almost every living organism. Precursor, or pre-tRNAs are cleaved by RNase ... illustrated the power of polycistronic gene organization, and its translation to CRISPR/Cas9 shows great...

  16. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ...the relevant tag and then introducing a specific ligand to mark the tag and whatever it is fused to for...expressed, protein levels will quickly recover once the ligand is removed. Degron tag systems include small molecule...

  17. All in a Twist: dsRNA

    Type
    Blog Post
    ...previous chemical versions with toxicity to many organisms. Fig. 1: Exogenous sources of dsRNA ...cellular dysregulation) is the mitochondria. These organelles have their own circular genomes which can produce...

  18. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...mammalian cell lines, Drosophila cells, and C. elegans. In addition to generating fluorescent protein .../Cas9 in human stem cells to illuminate cell organization." bioRxiv (2017): 123042. Additional Resources...

  19. RNA Extraction Without A Kit

    Type
    Blog Post
    ...proteins are retained in the interphase and bottom, organic layer. The top aqueous layer is extracted and RNA...layer to avoid carryover of DNA from the bottom, organic layer. Another trick is to precipitate the RNA ...
Showing: 321 - 340 of 601 results