...and improved. The CRISPR posts written by our guest bloggers, the experts in the CRISPR/Cas field, have ...past year. A quick glance shows that interest in CRISPR continues to grow as the genome editing technique...Popular Posts of 2014 How to Design Your gRNA for CRISPR Genome Editing By John Doench (Guest Blogger) ...Plasmids 101: Yeast Vectors By Marcy Patrick CRISPR-Cas9 FAQs Answered! By Caroline LaManna Plasmids...

...TZAP Hot Plasmid 4: CRISPR tools for use in plants.  Listen to the segment on CRISPR tools for plants. ...biology, plasmids for enhancing iPSC production, and CRISPR tools for plants. You can find additional hot plasmids... crispr...

...and Flexibility to CRISPR Editing by Jennifer Tsang Prime editing is a new CRISPR technique from David...on various topics in biology over the past year: CRISPR, optogenetics, chemogenetics, plasmids, fluorescent... the year. 10. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, ... lab that gives more control and flexibility to CRISPR editing. Using a fusion between Cas9 and reverse... recent plasmid blog posts Find the most recent CRISPR blog posts Resources at Addgene Browse the repository...

...for 1-3 days. There’s also a CRISPR for that! CRISPR interference (CRISPRi) targets a catalytically dead...little while Yes, there’s a CRISPR for that, too! CRISPR activators (CRISPRa) are transcriptional activators...approach - read more about them on our CRISPRa blog! Fig. 1 CRISPR activator and repressor mechanisms...knock out the gene for your protein of interest. CRISPR-Cas9 and a custom gRNA is all you need to generate... Promoters Plasmid 101: Repressible Promoters CRISPR 101: Which Cas9 Do I Choose for my Experiment ...resources on the Addgene website Promoter Guide CRISPR Guide Lentiviral Guide  ...double strand break. Transient introduction of CRISPRi or inducible expression (more on this below) will...

...Modifying the CRISPRi system for multidirectional control The CRISPR interference (CRISPRi) model consists...Addgene: Plasmids for CRISPRi are described here: http://www.addgene.org/crispr/qi/ Plasmids described...Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds." Adam has also created a video to...metabolic biofactories by demonstrating the use of CRISPR-based RNA scaffolds to mimic natural transcriptional...AdamChinFatt     References Qi, L. S. et al. Repurposing CRISPR as an RNA-guided platform for sequence-specific...Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell, 160(1), 339-350 (2015). PubMed... crispr...

...is not scalable for every locus...unlike CRISPR. Early CRISPR labeling techniques allowed researchers ...fluorescent CRISPR labeling, they took a new approach: labeling the gRNA itself. In their CRISPRainbow paper...Colorful CRISPR technologies are helping researchers visualize the genome and its organization within...visualize genomic loci in vivo The flexibility of CRISPR has improved our ability to target almost every... work of Chen et al., Thoru Pederson’s lab used CRISPR to label multiple loci in distinct colors. To create...Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System.” Cell 155 (2013): 1379-1491. Pubmed... at Addgene. 2. Ma, Hanhui, et al. “Multicolor CRISPR labeling of chromosomal loci in human cells.” Proc...

...blog CRISPR 101: Cytosine and Adenine Base Editors Read about four base editor reporters CRISPR 101: ...Editing CRISPR 101: Cas9 Structural Biology Additional resources on Addgene.org Browse CRISPR plasmids...editing Get a broad overview with our CRISPR Guide Check out our CRISPR 101 ebook ...The first base editors revolutionized CRISPR gene editing. Cytosine base editors (CBEs) and adenine base...base editing tools First, let’s briefly review how CRISPR-derived base editing works in general. Base editors... on the outside of the enzyme. Figure 2: CRISPR R-loop. Created with BioRender.com. Base editors...stretch of ssDNA created by Cas9 binding. Pro tip! In CRISPR, the non-target strand is the DNA strand that does...

...here. Here's what you'll find in this post: CRISPR-Csm: A novel RNA targeting system Heat-inducible...N86/8R] ready-to-use antibody Hyperfolder YFP CRISPR-Csm: A novel RNA targeting system by: Susanna Stroik... a leg up on the alternatives - RNAi and Cas13. CRISPR-Csm doesn’t degrade proximal RNAs nor does it have... off-target effects, both of happen with Cas13. CRISPR-Csm degrades both nuclear and cytoplasmic mRNA ... the 11 candidate transcript targets analyzed. CRISPR-Csm presents an attractive alternative for nuclear-RNA...Adapted from Figure 3 Colognori et al 2022.   Find CRISPR Csm plasmids here! Colognori D, Trinidad M, Doudna...Doudna JA. Precise transcript targeting by CRISPR-Csm complexes. Nat Biotechnol. 2023 Jan 23. doi: 10.1038...

... TALEN kit to CRISPR/Cas9? When would you choose one or the other? Musunuru: The CRISPR/Cas9 system is...system for CRISPR/Cas9 (also available from Addgene) only has two plasmids. In many cases, CRISPR may be ... now makes possible, and how it compares to his CRISPR/Cas9 system. Addgene: For people unfamiliar with...be more efficient. You might often start with CRISPR/Cas9, but TALENs do have more flexibility in terms...data to date suggest TALENs may be cleaner than CRISPR/Cas9. Reference: Quirong Ding et al. "A TALEN ...

...the Addgene blog CRISPR101: Editing the Epigenome  Mapping the 4D Nucleome with CRISPR/Cas9 Resources ...introduce you to a simpler, yet more effective, CRISPR-based epigenetic approach (spoiler alert: it’s ...dioxygenase  When I started to work on building a CRISPR/dCas9-based tool for DNA demethylation in 2015,...al. 2018, Kaas et al. 2013). Figure 2: CRISPR TET-based epigenetic editing. In reality, editing...Moshe Szyf at McGill University, working to develop CRISPR-based tools for epigenetic editing.   References...Resources at Addgene.org Read our CRISPR Guide to Epigenetics References Charlton J, Jung EJ, Mattei AL et al...targeted steric blockage of DNA methyltransferase with CRISPR/dCas9. Nat Commun 12, 5711. https://doi.org/10.1038...