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...non-targeting guides, when both are analyzed using the same false-discovery rate cutoff.  Adding safe-targeting...genome-wide CRISPR screens are prone to some of the same flaws as RNAi screens, such as non-specific toxicity...

...super-resolution STORM imaging, and electron microscopy. Sample preparation for these techniques sometimes makes...smFPs retain their ability to be detected after sample preparation better than standard proteins tags ...

...machinery in order to replicate—but not always to the same extent. As described in our previous Origin of Replication...proteins, it can be regulated indirectly via the same mechanisms that affect DnaA and IHF. RepA also controls...

...#211756 (left panel) or #211757 (middle panel). Samples were also immunoblotted against vinculin as a loading...during action potentials of fast-spiking neurons. The same A-to-D mutation in the related voltage indicator...

...Support is essential for success. For possibly this same reason, it can be one of the more constant and safe...many different backgrounds and realizing that the same approach does not work for everyone. The benefit...

...possible to set up the BP and LR reactions in the same tube, speeding up the cloning of the attB-PCR products...multiple DNA fragments into many vectors at once in the same tube. You can clone up to 4 DNA fragments, in a ...

...materials available from commercial repositories and the same cell line from a colleague?”. The main difference...) profiling can be used to ID the cell line. The same test is so good that it is used as a forensic technique...

.... Know your target cells Labs tend to follow the same general procedure for lentiviral infections, produce...but also between different lots of serum from the same source. Labs that routinely produce lentivirus may...

...What is FRET? In their JCB 2003 paper, Sekar and Periasamy defined FRET as "a distance-dependent physical...341-50. doi: 10.1002/bies.201100098. Sekar RB, Periasamy A. Fluorescence resonance energy transfer (FRET...

...orange fluorescent aptamers Corn was described in the same study as Red Broccoli and Orange Broccoli, two more...PMC5679246. Strack, Rita L., Matthew D. Disney, and Samie R. Jaffrey. "A superfolding Spinach2 reveals the...

... as a proxy for your edit of interest. Using the same gRNA and donor, you can deliver a silent mutation...to compare the sequencing reads from your edited sample to an untreated population. Software such as CRISPResso...

...nbt0702-697 Hirsch ML, Agbandje-McKenna M, Jude Samulski R (2010) Little Vector, Big Gene Transduction:...Bellon I, Yin C, Chavala S, Pryadkina M, Richard I, Samulski RJ (2013) Oversized AAV Transductifon Is Mediated...

...Cells often express multiple integrins that bind the same ligand, making it difficult to dissect the function...extracellular matrix. During immune responses, this same conformation encourages immune cell adhesion and...

... proteins are also a better choice for thicker samples. Another solution to avoid autofluorescence is ...reaction and hence, there is no need to excite the sample. Although bioluminescent probes are usually dim...

...activate or repress target genes in plants using the same general experimental design principles developed... expression of both Cas9 and the gRNA off of the same plasmid. Delivering CRISPR components to plant cells...

...doi.org/10.1074/jbc.M101559200 Wu, Z., Asokan, A., & Samulski, R. J. (2006). Adeno-associated virus serotypes....2006.05.009 Wu, Z., Miller, E., Agbandje-McKenna, M., & Samulski, R. J. (2006). Α2,3 and α2,6 N-Linked Sialic Acids...

...system to GAL4/UAS that functions in essentially the same manner, with LexA binding to the lexA operator (...desirable to control or look at multiple genes at the same time.  The 3 binary systems listed above are orthogonal...

...at the same location that their host colonies grew. If your cells of interest stick to the same location...

... about these methods is that they all follow the same general cyclical process (Figure 2). The differences...codons (different codon sequences that code for the same amino acid) for various amino acids. Essentially...

...commonly used for every cell culture. Reusing the same bottle of serum again and again for each subculture... changed for at least two or three days before sampling (Uphoff and Drexler, 2011). Culturing on agar...