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  1. The Challenges of Cell Culture

    Type
    Blog Post
    ...materials available from commercial repositories and the same cell line from a colleague?”. The main difference...) profiling can be used to ID the cell line. The same test is so good that it is used as a forensic technique...

  2. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    .... Know your target cells Labs tend to follow the same general procedure for lentiviral infections, produce...but also between different lots of serum from the same source. Labs that routinely produce lentivirus may...

  3. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ... Gene isoforms refer to mRNAs generated from the same gene but with different untranslated or coding regions...transcriptional start sites, all of which yield the same BDNF protein. To demonstrate the specificity that...

  5. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...super-resolution STORM imaging, and electron microscopy. Sample preparation for these techniques sometimes makes...smFPs retain their ability to be detected after sample preparation better than standard proteins tags ...

  6. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...What is FRET? In their JCB 2003 paper, Sekar and Periasamy defined FRET as "a distance-dependent physical...341-50. doi: 10.1002/bies.201100098. Sekar RB, Periasamy A. Fluorescence resonance energy transfer (FRET...

  7. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...activate or repress target genes in plants using the same general experimental design principles developed... expression of both Cas9 and the gRNA off of the same plasmid. Delivering CRISPR components to plant cells...

  8. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...Cells often express multiple integrins that bind the same ligand, making it difficult to dissect the function...extracellular matrix. During immune responses, this same conformation encourages immune cell adhesion and...

  9. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ... as a proxy for your edit of interest. Using the same gRNA and donor, you can deliver a silent mutation...to compare the sequencing reads from your edited sample to an untreated population. Software such as CRISPResso...

  11. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...orange fluorescent aptamers Corn was described in the same study as Red Broccoli and Orange Broccoli, two more...PMC5679246. Strack, Rita L., Matthew D. Disney, and Samie R. Jaffrey. "A superfolding Spinach2 reveals the...

  12. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ... proteins are also a better choice for thicker samples. Another solution to avoid autofluorescence is ...reaction and hence, there is no need to excite the sample. Although bioluminescent probes are usually dim...

  14. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...system to GAL4/UAS that functions in essentially the same manner, with LexA binding to the lexA operator (...desirable to control or look at multiple genes at the same time.  The 3 binary systems listed above are orthogonal...

  18. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...are working with proto-oncogenes or with human samples that have not been tested for HIV.  If not using...ribosome entry site (IRES) and expressed under the same promoter. Alternatively, your gene and the marker...

  19. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...validation screen using selected gRNAs extracted from the same or another library. These different approaches could...Listed you can also easily extract gRNAs for the same gene from several libraries, and identify those ...
Showing: 301 - 320 of 581 results