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We narrowed to 602 results for: sam

Showing: 301 - 320 of 602 results

  1. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...often: Q1: Are retroviruses and lentiviruses the same thing? Can I package lentivirus with retroviral ...both lentiviruses and gamma-retroviruses use the same genes for packaging (that's gag, pol, and env), ...

  2. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...plasmids, all of which need to be represented in the same proportion after amplification as they were prior...visualization) that confirm diversity of the library sample, and any other information or scripts provided ...

  3. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...amino acids and three stop codons, meaning that the same amino acid can be encoded by more than one codon...organisms. There are several codons that encode the same amino acid. Each organism, however, may preferentially...

  4. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ... Gene isoforms refer to mRNAs generated from the same gene but with different untranslated or coding regions...transcriptional start sites, all of which yield the same BDNF protein. To demonstrate the specificity that...

  5. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...different from other plasmids? If we all use the same molecular code, can I transfect any old bacterial...it’s pretty incredible that my cells “speak” the same molecular language as the tree outside my window...

  7. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...super-resolution STORM imaging, and electron microscopy. Sample preparation for these techniques sometimes makes...smFPs retain their ability to be detected after sample preparation better than standard proteins tags ...

  8. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...machinery in order to replicate—but not always to the same extent. As described in our previous Origin of Replication...proteins, it can be regulated indirectly via the same mechanisms that affect DnaA and IHF. RepA also controls...

  9. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...#211756 (left panel) or #211757 (middle panel). Samples were also immunoblotted against vinculin as a loading...during action potentials of fast-spiking neurons. The same A-to-D mutation in the related voltage indicator...

  10. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...Support is essential for success. For possibly this same reason, it can be one of the more constant and safe...many different backgrounds and realizing that the same approach does not work for everyone. The benefit...

  11. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...possible to set up the BP and LR reactions in the same tube, speeding up the cloning of the attB-PCR products...multiple DNA fragments into many vectors at once in the same tube. You can clone up to 4 DNA fragments, in a ...

  12. The Challenges of Cell Culture

    Type
    Blog Post
    ...materials available from commercial repositories and the same cell line from a colleague?”. The main difference...) profiling can be used to ID the cell line. The same test is so good that it is used as a forensic technique...

  13. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    .... Know your target cells Labs tend to follow the same general procedure for lentiviral infections, produce...but also between different lots of serum from the same source. Labs that routinely produce lentivirus may...

  14. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...What is FRET? In their JCB 2003 paper, Sekar and Periasamy defined FRET as "a distance-dependent physical...341-50. doi: 10.1002/bies.201100098. Sekar RB, Periasamy A. Fluorescence resonance energy transfer (FRET...

  15. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ... as a proxy for your edit of interest. Using the same gRNA and donor, you can deliver a silent mutation...to compare the sequencing reads from your edited sample to an untreated population. Software such as CRISPResso...

  16. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...orange fluorescent aptamers Corn was described in the same study as Red Broccoli and Orange Broccoli, two more...PMC5679246. Strack, Rita L., Matthew D. Disney, and Samie R. Jaffrey. "A superfolding Spinach2 reveals the...

  18. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ... proteins are also a better choice for thicker samples. Another solution to avoid autofluorescence is ...reaction and hence, there is no need to excite the sample. Although bioluminescent probes are usually dim...

  19. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...Cells often express multiple integrins that bind the same ligand, making it difficult to dissect the function...extracellular matrix. During immune responses, this same conformation encourages immune cell adhesion and...

  20. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...activate or repress target genes in plants using the same general experimental design principles developed... expression of both Cas9 and the gRNA off of the same plasmid. Delivering CRISPR components to plant cells...
Showing: 301 - 320 of 602 results