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  1. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...cleavage system. LwaCas13a is also amenable to multiplexing; expressing 5 gRNAs simultaneously produces ... Featured Topic Page Learn about Anti-CRISPRs Multiplex Genome Editing with Cpf1 Resources on Addgene.org...

  2. Bioinformatics at Addgene

    Type
    Blog Post
    ...inherent in Nextflow’s design, we were free to use multiple programming languages (Python, Perl, Java, R, ... adaptable property gave us the freedom to use multiple open-source sequence assembly tools at the appropriate...

  3. New CRISPR Tools: Cas7-11 and PASTE

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    Blog Post
    ... the more complex Class I systems that contain multiple subunits, but the more they worked with it, the...high-fidelity rate. Both Cas7-11 and PASTE open up multitudes of possibilities at the bench and potentially...

  4. Plasmids 101: E. coli Strains for Protein Expression

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    Blog Post
    ...reductase to faciliate proper folding; may increase multimer formation Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII ... Proteins which contain hydrophobic regions or multiple disulfide bonds may aggregate and become insoluble...

  6. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...be packaged in AAV vectors, potentially with multiplexed targets.   Figure 4: CHARM uses a fusion...  Multiple Authors...

  8. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ... scrambling. Everything we had set up involved multiple people in tight locations. Conference rooms for...to the talent’s shirt. But knowing I would have multiple interview subjects, I positioned a boom mic just...

  9. Antibodies 101: Beyond Surface Labeling

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    Blog Post
    ...These methods can include labeling markers with multiple proteins/antibodies, targeting markers found within...Otherwise, your secondary antibody will bind to multiple primary antibodies, yielding a false positive ...

  10. CRISPR 101: Homology Directed Repair

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    Blog Post
    ...Pfeiffer P, et al. Mutagenesis (2000). PubMed. Multiple Pathways of Recombination Induced by Double-Strand... Multiple Authors...

  13. Transferable Skills Guide: Conflict Resolution

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    Blog Post
    ...every communication that gets sent out through multiple channels is not reasonable or sustainable. That...will be gray areas that either a) overlap with multiple teams or b) fall into a gap between teams that...

  14. Five Popular Model Organisms

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    Blog Post
    ...organisms, they can also be used to easily screen multiple therapeutic compounds in one experiment. Zebrafish...making it easy to conduct large experiments over multiple generations. Similarly to other model organisms...

  16. Antibodies 101: The Basics of Western Blotting

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    Blog Post
    ...and reprobed with different antibodies allowing multiple targets to be assessed from a single western.  ...used to visualize the proteins. In this set-up, multiple secondary antibody molecules bind the primary ...

  17. How to Design Your gRNA for CRISPR Genome Editing

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    Blog Post
    ... any gene of interest, one should require that multiple gRNAs of different sequences give rise to the ... in the interpretation of results by requiring multiple sequences targeting a gene to score in order to...

  18. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...goal of isolating single cell clones to use for multiple downstream experiments. You only successfully ...most commonly used Cas, spCas9, has gone through multiple rounds of optimization which have led to more ...

  19. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ... genome amplification (by a mechanism known as multiple displacement amplification) even in the presence... of 10-20 colonies using primers bordering the multiple cloning site of the vector. In order to reduce...
Showing: 301 - 320 of 544 results