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  1. Antibodies 101: Validation

    Type
    Blog Post
    ... does not mean that is all it will bind to - particularly when you start using complex samples such as... the antibody webpages. These outlets can be particularly useful for sharing negative data. Negative data...

  2. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ... different colors. This lends flow cytometry particularly well to complex experiments looking at a variety...from the use of secondary antibodies. It’s also particularly useful for intracellular staining where binding...

  3. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...bacteria or cell-free systems. The approach is particularly useful for producing drug precursors, biofuels...works in both mammalian systems and Drosophila. Similarly, the Multi-Kingdom (MK) Golden Gate system was...

  4. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...Initial photoactivatable CRISPR systems published in early 2015 focused on using light to control transcription...Moritoshi Sato’s lab at the University of Tokyo and Charles Gersbach’s lab at Duke University developed similar...

  5. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...enough to get good separation in your ladder, particularly in the sizes around your protein of interest... leftover sample) to fill the relevant wells. Similarly, if you load a gel and then forget to start it...

  6. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...provides improvements to classical cloning methods, particularly for complex transgenesis constructs. Kirchmaier...terminator). Next, up to six Level 1 modules can be similarly assembled into a Level 2 vector, thus forming ...

  7. Antibodies 101 Series on TikTok

    Type
    Blog Post
    ... Addgene We'll be posting these animations regularly from now until May, so follow us to get updates...

  8. CRISPR 101 eBook...en español

    Type
    Blog Post
    ... científica profesional, L. Denise Roche, para editarla. Estamos orgullosos de anunciar que CRISPR 101...

  15. CRISPR Cheat Sheet

    Type
    Blog Post
    ... Sheet here!   CRISPR Cheat Sheet Clustered Regularly Interspaced Short Palindromic Repeats: Sequences...

  20. Countdown to Halloween @Addgene

    Type
    Blog Post
    ...that our costume contests were indeed occurring earlier but that the photo evidence was destroyed to protect...
Showing: 301 - 320 of 560 results