...fluorescent proteins (FPs) have become one of the most indispensable tools in modern biological research...probe (be it an organic dye or FP) is one of the most important steps in the design of an imaging experiment...soon as possible after submission. Using FPbase The most obvious use case for FPbase is finding information...

... plasmids. These libraries are some of Addgene’s most exciting and versatile offerings! We recently re-amplified...protocol, but the overall workflow is similar for most libraries. Here are the key points you’ll need to...Figure 3A). Addgene finds that this representation is most easily understood visually. As an example, the curves...

...presented chronologically. With a resume however, most often people choose a reversed chronological style...often I see resumes from good candidates that I almost missed because I had to “dig” hard for the information... in the News Talk to your career services office Most universities actually have career services offices...

...Addgene’s content team, we — Jo, Brook, and Angela — most often think about accessibility in terms of the ...working together to share information. Sometimes it is most helpful to use a lot of jargon and have a technical...takeaway.  In this image, we thought it would be most helpful to highlight the technical details of collecting...

...genetic modification by homologous recombination. In most recombineering strategies, only 3-4 phage proteins...a whopping 144 kb from a bacterial artificial chromosome in just one reaction. Using other methods, such... which are recommended for plasmids up to 25 kb, most cloners should be fine, especially using plasmids...

...classroom cannot offer. Furthermore, companies across most industries tend to hire their former interns to ...assess your eligibility, interest, and career fit. Most internships share a common set of criteria such ...background, and lighting for a smooth performance. Most importantly, make certain that everyone you live...

...? There have been several systems to do this but most are labor-intensive, require multiple fluorescent...They continued to use GEmCherry2 to identify the most efficient sgRNAs to target a specific locus to create...efficiency. So if you are looking to identify the most effective gRNA and enrich cells for your CRISPR ...

...injected 1e+13 vg per mouse to get phenotypes (that’s almost 100ul/animal for the PHP.eBs that we have in house...expression is when injecting CAP-B10 or CAP-B22 in marmosets, from an individual to another individual perspective...CAP-B10 and CAP-B22 were tested individually in 4 marmosets each. Brain potency for each individual is reported...

...each other until you are ready to separate them. Most proteins are not likely to remain attached to the...want to break them. Crosslinking is necessary for most ChIP experiences, but the increased binding can ...and complex, you may be thinking, well, these are mostly fairly standard molecular and cell biology techniques...

... write them on a board to see which ones get the most hits. Commit to a meeting schedule and meet regularly...ready for the discussion with resources. One of the most common questions I get is, “What are we going to...– compete for fastest to set up a coffee date or most cards collected. The next blog in this series will...

..., including dendritic and axonal extensions and, most importantly, any connections the neurons make to...capable of spreading across synaptic connections. Most famous is the Rabies Virus (RABV) which moves retrogradely...  Commonly used serotypes include AAV1 – 9, with most of these serotypes capable of entering neurons (...adeno-associated viral vector serotypes 1, 2, 5, 8 and 9 in marmoset, mouse and macaque cerebral cortex, Neurosci Res...

...     Of course, the lack of an Fc portion means that most of the common anti-IgG secondary antibodies you ...antibody as well.  Next, Fab and Fab’ fragments are almost interchangeable, but the extra bit of heavy chain...convenient site for conjugation.  Finally, Fvs are the most amenable to recombinant expression and will have...

... expression, you are almost certainly working with an endogenous locus. The most straightforward way to...function at the post-translational level, unlike most of the other mechanisms described. Fig. ...

...Well, that’s just for DNA – right? RNA performs almost all of its biological functions in our cells in...transcription or as inverted repeats. These elements are almost always epigenetically silenced, but dysregulation...RNases in mammalian cells (RNase H1 and H2) handle most of these intermediates, with each one acting on ...

... RNA samples. RNA is inherently less stable than most macromolecules used in scientific research such ...with post translational modifications. One of the most common of these is 2’-O-methylation, where a methyl...major drawback of CRISPR-Cas9 technology is that most expression systems are constantly “on”. There is...

...gives you access to hard data about what steps are most likely to fail. Our laboratory manager would definitely...interested to see which part of her procedures are the most failure prone. In an academic lab, a similar situation...capital cost of getting up to speed with a setup is almost always worth it. Because labels are applied to ...

...cloning experiments a little easier. One of the most time-consuming aspects of traditional cloning is...resistant to CcdB, as the native ccdA will be present. Most common cloning strains of E. coli do not contain...ever more important to ensure that you choose the most appropriate method for your own experiments. Please...

...systems, making it well suited for AAV packaging. Most importantly, this CasMINI system was shown to be...compact genome editors and a gRNA down-sized by almost 40%. The activity of Cas12f1 with these modified...of the system to optimize its outcomes. In their most recent work, the Liu Lab boosts the efficiency of...

...facilitate its detection. Fluorescent proteins are most widely used tags because they emit endogenous fluorescence...fluorescent protein (GFP) and mCherry, two of the most common tags currently used, are approximately 240...larger proportion on the membrane in mice neurons, most of them were still cytoplasmic. Use of ‘small’ tags...

...number by introducing genes of interest into the chromosome. But this has its own set of problems. Rapidly...is expressed from a plasmid as opposed to the chromosome. Let’s take a look at how the stabilized promoters...levels of expression between a plasmid and the chromosome The team also showed that the levels of gene ...