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We narrowed to 610 results for: sam

Showing: 281 - 300 of 610 results

  1. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...between the sites. If a DNA sequence is floxed in the same orientation, the SSR will excise the DNA fragment...reporter with one loxP site and one lox511 site in the same orientation, and placing the additional loxP and...

  2. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...answers uninteresting, the panel members all say the same thing and one panel member dominates the discussion... for an intimate event, put the panelists on the same level as the audience. In my experience the best...

  4. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...vivo. The general process of these screens is the same: Deliver CRISPR library to cells of interest. Select...Low, S. H., François, A., Suárez-Amarán, L., & Samulski, R. J. (2022). AAV vectors: The Rubik’s cube of...

  5. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ..., so the amount of cloning required is about the same between insect and mammalian systems. Baculovirus...P1 virus, so that if you ever need to remake the same virus, you can skip the first round of production...

  6. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...known titer on every qPCR run. Note that the rAAV sample has to be digested with DNase before undergoing...Grimm, D., Kern, A., Pawlita, M., Ferrari, F., Samulski, R., & Kleinschmidt, J. (1999). Titration of AAV...

  7. Pooled Library Amplifications

    Type
    Blog Post
    ...library from Addgene, they’ll need to amplify the sample for further downstream applications. As individual... all of the bacteria can grow and compete in the same container, making it easier for a smaller, recombined...

  8. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...mammalian cells, finding xCas9 3.7 displayed about the same editing rate as SpCas9 at an NGG PAM. When they ...Adenine base editor xCas9 3.7-ABE also follows the same pattern, with improved editing at an NGG PAM (69...

  9. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ... this purpose.Learn about the effects of CO2 on sample pH Cell morphology: Shape and size matter You should...contaminated.Learn how Addgene uses barcodes to track samples Keep track of passage numbers Keep an eye on the...

  16. MXS Chaining

    Type
    Blog Post
    ...e.g., a set of various mammalian promoters), the same cloning procedure can be used to incorporate each... individual plasmids and are each flanked by the same multiple cloning site (MCS). Modules are assembled...

  17. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...primary antibody-nanobody mix can be added to your sample, thus eliminating the secondary antibody incubation...568, or Alexa Fluor 647 before application to the sample. Staining with this multi-color staining workflow...

  19. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...retinal cells - AAV - were becoming available. At the same time advances in human genetics led to the identification...24664759. PubMed Central PMCID: PMC4267879. 10. Samulski RJ, Muzyczka N., 2014. AAV-Mediated Gene Therapy...

  20. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...sequences of guides across 8 CRISPR libraries. Samples are divided into those of African ancestry(orange... on these and any other CRISPR screens using the same reference genomes and libraries. Further complicating...
Showing: 281 - 300 of 610 results