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  1. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...known titer on every qPCR run. Note that the rAAV sample has to be digested with DNase before undergoing...Grimm, D., Kern, A., Pawlita, M., Ferrari, F., Samulski, R., & Kleinschmidt, J. (1999). Titration of AAV...

  2. Pooled Library Amplifications

    Type
    Blog Post
    ...library from Addgene, they’ll need to amplify the sample for further downstream applications. As individual... all of the bacteria can grow and compete in the same container, making it easier for a smaller, recombined...

  3. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ... this purpose.Learn about the effects of CO2 on sample pH Cell morphology: Shape and size matter You should...contaminated.Learn how Addgene uses barcodes to track samples Keep track of passage numbers Keep an eye on the...

  4. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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    Blog Post
    ...mammalian cells, finding xCas9 3.7 displayed about the same editing rate as SpCas9 at an NGG PAM. When they ...Adenine base editor xCas9 3.7-ABE also follows the same pattern, with improved editing at an NGG PAM (69...

  9. Uncovering Ancestral Bias in CRISPR Research

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    Blog Post
    ...sequences of guides across 8 CRISPR libraries. Samples are divided into those of African ancestry(orange... on these and any other CRISPR screens using the same reference genomes and libraries. Further complicating...

  10. MXS Chaining

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    Blog Post
    ...e.g., a set of various mammalian promoters), the same cloning procedure can be used to incorporate each... individual plasmids and are each flanked by the same multiple cloning site (MCS). Modules are assembled...

  11. AAVs in Retinal Gene Therapy

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    Blog Post
    ...retinal cells - AAV - were becoming available. At the same time advances in human genetics led to the identification...24664759. PubMed Central PMCID: PMC4267879. 10. Samulski RJ, Muzyczka N., 2014. AAV-Mediated Gene Therapy...

  12. Plasmids 101: Secondary Nanobody Toolbox

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    Blog Post
    ...primary antibody-nanobody mix can be added to your sample, thus eliminating the secondary antibody incubation...568, or Alexa Fluor 647 before application to the sample. Staining with this multi-color staining workflow...

  13. Tips for Screening with Yeast Two Hybrid Systems

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    Blog Post
    ...libraries of bait and prey against each other at the same time in order to try and access the entire interactome...the protein partner or the UAS/reporter gene, the same bait and prey libraries can be screened using both...

  14. Your Lentiviral Plasmid FAQs Answered

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    Blog Post
    ...often: Q1: Are retroviruses and lentiviruses the same thing? Can I package lentivirus with retroviral ...both lentiviruses and gamma-retroviruses use the same genes for packaging (that's gag, pol, and env), ...

  15. To Codon Optimize or Not: That is the Question

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    Blog Post
    ...amino acids and three stop codons, meaning that the same amino acid can be encoded by more than one codon...organisms. There are several codons that encode the same amino acid. Each organism, however, may preferentially...

  16. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...Support is essential for success. For possibly this same reason, it can be one of the more constant and safe...many different backgrounds and realizing that the same approach does not work for everyone. The benefit...

  17. Viral Vectors 101: Preparing Pooled Libraries

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    Blog Post
    ...plasmids, all of which need to be represented in the same proportion after amplification as they were prior...visualization) that confirm diversity of the library sample, and any other information or scripts provided ...

  18. The Challenges of Cell Culture

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    Blog Post
    ...materials available from commercial repositories and the same cell line from a colleague?”. The main difference...) profiling can be used to ID the cell line. The same test is so good that it is used as a forensic technique...

  19. Getting the Most from Your Lentiviral Transduction

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    Blog Post
    .... Know your target cells Labs tend to follow the same general procedure for lentiviral infections, produce...but also between different lots of serum from the same source. Labs that routinely produce lentivirus may...

  20. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...machinery in order to replicate—but not always to the same extent. As described in our previous Origin of Replication...proteins, it can be regulated indirectly via the same mechanisms that affect DnaA and IHF. RepA also controls...
Showing: 281 - 300 of 581 results