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  1. CRISPR 101: Cas9 vs. The Other Cas(s)

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    Blog Post
    ...degradation. Cas3 DNA digestion can proceed up to multiple kilobases, but only in one direction. Cas3 is ...used in lieu of siRNA or shRNA for efficient, multiplexable RNA knockdown. Cas13 has also stirred up interest...

  4. Mouse Modeling, Part 2: Breeding and Crossing Mice

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    Blog Post
    ...genome, they are not commonly used to introduce multiple mutations. This is because as more mutations are...challenge that may arise when crossing mice with multiple mutations is if the mutations are on the same ...

  5. Viral Vectors 101: Calcium Sensors

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    Blog Post
    ...binding of the biosensor to endogenous calmodulin. Multiplexing Are you combining your calcium reading with ...you need to free up crowded optical space for multiplexing, GECO sensors are available in green, blue, ...

  6. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ...pre-designed sets, so identical searches can return multiple answers. You might want to take advantage of their...SplitSet finds optimal fusion sites to split it into multiple fragments. You can define the number of fragments...

  7. Hot Plasmids and Viral Preps - March 2021

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    Blog Post
    ...elegant system that allows for the assembly of multiple DNA fragments efficiently and accurately in a ...system will allow investigators to rapidly create multiple synthetic chromatin-modifying proteins quickly...

  8. Adenoviral Vector Production and Troubleshooting

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    Blog Post
    ...which time the cells are scraped, and lysed by multiple freeze-thaw cycles. The resultant lysate is your...cytopathic effect.” Amplification can be repeated multiple times (2-4 rounds) at increasing scale over the...

  10. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

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    Blog Post
    ...editing efficiency of prime editing, whereby a multitude of configurations can be used to introduce specific...efficiencies for diverse prime editing systems in multiple cell types. Cell, 186, 1–17. https://doi.org/10.1016...

  11. 10 Basic tips for mammalian cell culture

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    Blog Post
    ...fast-growing cell lines (such as HEK293, which multiplies every 16 hours). So if you, for example, split... its passage number. Especially in a lab where multiple people are working on different things, this will...

  12. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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    Blog Post
    ...According to this logic, ω-dCas9 variants recognizing multiple PAMs should have a fitness advantage over proteins... and CAA PAMs, they saw that xCas9 3.7 cleaves multiple PAMs at a higher efficiency than SpCas9: NGT (...

  13. Google Forums Round Up: First Impressions of NgAgo

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    Blog Post
    ..., the widespread success of CRISPR-Cas9 across multiple model organisms (3) lies in the versatility of...which could be less desirable if one has to target multiple sites in a single experiment. Also, the NGG PAM...

  14. A Career in Grants: How To Become a Grant Professional

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    Blog Post
    ...  Skills such as research, collaboration across multiple disciplines, writing, project management, and ...knowledge of the 8 core competencies through a multiple choice exam.  The bottom line on being a grants...

  15. Plasmids 101: Choosing an Antibiotic Resistance Gene

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    Blog Post
    ...attention to antibiotic selection. If you feel like multiple of the above antibiotics would work for you, they...Antibiotic Resistance Stability Time Saving?  Works for multiple organisms? Compatible with most bacteria? No satellite...

  16. Plasmids 101: Protein tags

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    Blog Post
    ...depicted in the accompanying cartoon, they have a multitude of uses including (but not limited to) purification...localizes your protein to the nucleus? Or you want multiple rounds of purification to get your protein as ...

  18. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...interdisciplinarity index of research for single or multiple disciplines – you can select the disciplines and...networking, visual appeal, instant sharing across multiple other sites), I created a new platform for sharing...

  20. Bioinformatics at Addgene

    Type
    Blog Post
    ...inherent in Nextflow’s design, we were free to use multiple programming languages (Python, Perl, Java, R, ... adaptable property gave us the freedom to use multiple open-source sequence assembly tools at the appropriate...
Showing: 281 - 300 of 544 results