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  2. AAV ddPCR Titration

    Type
    Protocol
    ...AAV titer range of 5E+12–5E+13 GC/mL, where GC is genome copies and generally represents the physical number...

  4. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...be delivered to cells both transiently and for genomic integration. Lab-engineered viruses will package...package your genetic cargo in lieu of their own genomes and deposit this cargo upon host infection. Packaging...

  8. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...knockdown, Tet and Cre-regulated expression, CRISPR genome editing, and more. Not surprisingly, we receive...: Download our eBook: Plasmids 101 Learn about genome-wide screening with CRISPR and Lentivirus Read ...

  10. Call for Guest Bloggers

    Type
    Blog Post
    ... Biogen interested in applying CRISPR for mouse genome editing. Post: Generating Mouse Models Using CRISPR...

  13. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...CNS). AAVs are popular tools because: 1) their genomes are easy to manipulate, 2) they have long-term ... it’s parent AAV.PHP.B: 1x1011 vs 1x1012 viral genomes (vg) per mouse. Figure 3 shows a gross comparison...

  14. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...-stranded intermediate into the single-stranded genome that's then packaged into AAV’s protein shell, ...: AAV titering by qPCR quantifies the number of genome-containing viral particles that are present in ...

  15. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ... sites, the phage integrates into the bacterial genome flanked by two new recombination sites (attL-left...fragments may be cloned: PCR fragments, cDNA or Genomic DNA and is available for all kind of organisms ...

  16. Editor's Choice, July 2016

    Type
    Blog Post
    ...with over 700 views) was an update on new CRISPR genome editing technology, Cpf1. Written by our Science...

  17. AAV Packaged on Request is Here!

    Type
    Blog Post
    ...purity testing, and sterility testing, plus viral genomic sequencing to confirm the identity of the packaged...

  19. CRISPR Library Amplification

    Type
    Protocol
    ...RNAs targeting different genes in an organism's genome, for example. Amplification is usually necessary...

  20. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...alone, and this is expanding as we obtain more genomic data about plasmids (Shintani et al., 2015). Now...22153351 Shintani M, Sanchez ZK, Kimbara K (2015) Genomics of microbial plasmids: classification and identification...
Showing: 281 - 300 of 534 results