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  1. Qi Lab CRISPR Page

    Type
    Collection
    ... CRISPR Qi Lab CRISPR Plasmids Qi Labs CRISPR Plasmids...Addgene You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts We have recently...CRISPR CRISPRi Cas9 gRNA plasmids from the Qi lab CRISPR...or human cells ( Qi et al. ). This CRISPR interfering system (CRISPRi), works independently of host cellular...uniqueness of CRISPRi, as compared to several recently published works on applying the wild-type CRISPR system...recently repurposed the bacterial immune system, CRISPR ( C lustered R egularly I nterspaced S hort P alindromic...genes. These plasmids are described in: Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific...

  2. Zebrafish Plasmid Collection

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    Collection
    ...Collection CRISPR Please refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology...examples of useful zebrafish CRISPR resources can be found below: UFlip CRISPR targeted integration vectors...for Targeted Integration Using CRISPR/Cas9 - Jeffrey Essner Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) expression...Joung Lab CRISPR/Cas9-based conditional mutagenesis in zebrafish - Wenbiao Chen Lab A CRISPR/Cas9 Vector...modified CRISPR-Cas9 system - Shuo Lin Lab Re-purposed Ac/Ds transgenic system enabling CRISPR/dCas9-interference...for the laboratory use of zebrafish. CRISPRz - A database of CRISPR/Cas9 target sequences that have been...morpholinos, zinc finger nucleases, TALENS, and CRISPR/Cas9-based gene editing make the zebrafish a stalwart...

  3. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Base...CRISPR plasmids that have been designed for use in mammalian systems. CRISPR... CRISPR Mammalian Expression CRISPR...CRISPR Plasmids: Mammalian Expression The following CRISPR plasmids have been designed for use in mammalian...mammalian expression systems. Cut Fully functional CRISPR/Cas enzymes will introduce a double-strand break (...Promoter Selectable Marker PI Publication Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit...->G (or T->C on the opposite strand) mutations. CRISPR-X, a base-editing system from the Bassik lab, uses...

  4. Guide RNA Expression Plasmids for Endogenous Zebrafish Genes

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    Collection
    ... CRISPR Joung Lab CRISPR Plasmids Zebrafish gRNA CRISPR-Cas/RGN expression plasmids...Zebrafish You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The Joung Lab...Lab recently described the use CRISPR-Cas/RNA-Guided Nuclease ( RGN ) technology to modify nine different...

  5. Genomic Deletions in Mammalian Cell Lines

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    Collection
    ... CRISPR References & Information Genomic Deletions in Mammalian Cell Lines CRISPR: Protocol...Mammalian Cell Lines Other Addgene Resources CRISPR Plasmids CRISPR Guide Addgene Blog Posts Addgene is proud...regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) 9 system may be re-purposed.... Perform maxi-prep for each CRISPR/Cas9 construct. Transfecting CRISPRs into Cells of Interest NOTE: ...using CRISPR/Cas9 CRISPR...Orkin's and Daniel Bauer's labs details the use of CRISPR/Cas9 to create genomic deletions in mammalian cell...of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9. Bauer DE, Canver MC, Orkin SH. J. Vis. Exp...

  6. CRISPR Plasmids - Yeast

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... CRISPR Yeast CRISPR Plasmids: Yeast Browse ... following CRISPR plasmids have been designed for use in yeast. Cut Fully functional CRISPR/Cas enzymes...CRISPR plasmids that have been designed for use in yeast. CRISPR...Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation ... Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand...selectable marker or cloning method. When using CRISPR, you will need to express both a Cas protein and...

  7. Jaenisch Lab CRISPR Plasmids

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    Collection
    ...Jaenisch Lab CRISPR Plasmids Available from Addgene You may also like... CRISPR Guide CRISPR Protocols gRNA... CRISPR Jaenisch Lab CRISPR Plasmids Jaenisch...gRNA Design Tools CRISPR Blog Posts CRISPR-on is an RNA-guided transcriptional activator system with two...CRISPR Cas9 gRNA plasmids from Dr. Rudolf Jaenisch's lab. CRISPR...: Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system...

  8. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

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    Blog Post
    ...increasing the size of the AAV donor template for CRISPR-mediated homology directed repairs (HDR). Split...sequential homology directed repairs (HDR) with CRISPR/Cas9. Donor A contains: 400bp homology arms to ...single step and serve as donors for two consecutive CRISPR-mediated HDR events which results in the integration....org/10.1172/jci34316 Bak RO, Porteus MH (2017) CRISPR-Mediated Integration of Large Gene Cassettes Using...

  9. FlyCRISPR

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    Collection
    ...also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts Harrison Lab - Wildonger... CRISPR flyCRISPR Lab CRISPR Plasmids flyCRISPR Plasmids Available from Addgene You may also...generating gRNAs 106.5 KB flyCRISPR website contains additional information CRISPR Optimal Target Finder ...Browse CRISPR Cas9 gRNA plasmids for drosophila research from the O'Connor-Giles, Wildonger, and Harrison...described in: Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease. Gratz SJ, Cummings AM...Wildonger Lab - O'Connor-Giles Lab email: flycrispr@gmail.com We have found that a variety of Cas9-mediated...

  10. Zhang Lab's CRISPR Frequently Asked Questions

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    Collection
    ... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang Lab CRISPR FAQs Zhang...Zhang Lab's CRISPR Frequently Asked Questions You may also like... Zhang Lab CRISPR plasmids General CRISPR...using the lab's CRISPR technology. Visit the Zhang Lab page and forum to learn more. CRISPR Design FAQs Homologous...the Bench FAQs CRISPR Design FAQs Should I use wildtype or double nickase for my CRISPR genome engineering...Dr. Le Cong, the first author of a seminal CRISPR article from Dr. Feng Zhang's Lab, has assembled a ... lab's CRISPR technology. Genome... CRISPR Plasmids Dr. Le Cong, the first author of one of the seminal articles from Dr. Feng Zhang's Lab...

  11. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    ...proteins to monitor the localization of these genes, CRISPR to disrupt their function, and C. elegans overexpression...peritoneal mesothelioma tumorigenesis. They plan to use CRISPR and lentiviral plasmids from Addgene to knockout...

  12. Church Lab CRISPR Plasmids

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    Collection
    ...Addgene You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts Bacteria and... CRISPR Church Lab CRISPR Plasmids Church Lab CRISPR...regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems that use short RNA...reagents for custom CRISPR mediated gene-targeting. A protocol for synthesizing gRNAs: hCRISPR gRNA synthesis...CRISPR Cas9 gRNA plasmids from Dr. George Church lab. CRISPR...acids. We have engineered the type II bacterial CRISPR system to function with custom guide RNA (gRNA)...Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems. Dicarlo JE, Norville JE, Mali P, Rios...

  13. Fujii Lab CRISPR Plasmids

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    Collection
    ... Fujii Lab CRISPR Plasmids Available from Addgene You may also like... CRISPR Guide CRISPR Protocols gRNA... Genome Engineering CRISPR Fujii Lab CRISPR Plasmids...CRISPR Cas9 gRNA plasmids from Dr. Hodaka Fujii's lab. Genome... gRNA Design Tools CRISPR Blog Posts Originally developed in 2009 (Hoshino & Fujii), the locus-specific...-binding molecules such as TAL proteins and the CRISPR system consisting of a catalytically inactive form....2018.03.150. PubMed . enChIP systems using different CRISPR orthologues and epitope tags. Fujita T, Yuno M,...Allele-specific locus binding and genome editing by CRISPR at the p16INK4a locus. Fujita T, Yuno M, Fujii ...

  14. CRISPR Plasmids - Plants

    Type
    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... CRISPR Plants CRISPR Plasmids: ...following CRISPR plasmids have been designed for use in plants. Cut Fully functional CRISPR/Cas enzymes...CRISPR expression plasmids that have been designed for use in plants. CRISPR...: Plants Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional... Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand...selectable marker or cloning method. When using CRISPR, you will need to express both a Cas protein and...

  15. Brzezinski Lab CRISPR Collection

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    Collection
    ...Collections Brzezinski Lab CRISPR Collection Brzezinski Lab CRISPR Collection The Brzezinski lab investigates...fusion for gene activation CRISPR/Cas9 Plasmid Variants Schematic of the CRISPR Collection. Plasmids 159654...Find CRISPR plasmids to study the regulatory networks involved in retinal development. Depositor...involved in retinal development the lab utilizes a CRISPR/Cas9 based approach to knockout, repress, and ...

  16. CRISPR Plasmids - Prime Edit

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Prime...Prime Edit CRISPR Plasmids: Prime Edit Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit...CRISPR nickase plasmids fused to reverse transcriptase for search and replace gene editing. CRISPR... CRISPR Prime... . Browse, sort, or search the tables below for CRISPR prime editing plasmids. Plasmids are available ...

  17. Addgene's eBook Collection

    Type
    Guide
    ...Close About Overlay Select CRISPR 101 January 2021 | Third Edition About CRISPR 101 January 2021 | ...Overlay Select CRISPR 101 en español January 2024 | First Edition, trans. About CRISPR 101 en español...experimental advice such as tips for planning your CRISPR experiment and designing your gRNA and how to use...traducción al español de nuestro libro electrónico, CRISPR 101. Close About Overlay Select Antibodies 101 ...

  18. Guide to Using Pooled Libraries

    Type
    Guide
    ...Using CRISPR/Cas9 Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening Pooled CRISPR Libraries...protocol. Read our CRISPR guide for more information on genome-wide screening with CRISPR/Cas9 . Browse our...of published selection mechanisms for lentiviral CRISPR libraries include: resistance to the anti-cancer...virus, make virus; this creates a pooled lentiviral CRISPR library. Apply pooled library to cells. DIVERGE... plasmids have been depleted by selection. In a CRISPR screen, negative screens are often used to identify...
Showing: 281 - 300 of 446 results