... view all kits. Kit Types Fluorescent Proteins Cloning Genome Engineering Pathways & ORFs Synthetic Biology...

...of one clone that are specific to a certain epitope, or polyclonal, meaning there are many clones that ...antibodies. Recombinant antibodies are created by cloning antibody genes from specific hybridoma cells into... bind to any given antigen. A mature B cell can clone itself to enable higher production of a single antibody...where each culture should produce the same antibody clone, or from plasmid-based expression. Monoclonal antibodies...

... Protocols Cloning by Limiting Dilution Isolating a Monoclonal Cell...transgene expression over time, as the lower expressing clones take over the polyclonal cell pool. Generating ...new window) or isolating individual cells with cloning cylinders. Workflow Timeline This protocol begins...

... plasmid as AAV. We currently do not offer DNA cloning services. To find out more about a specific plasmid...

...AscI at the 3' end. These inserts can then be sub-cloned into a series of expression vectors which have ...

... Anti-SARS-CoV-2 Nucleocapsid Protein [mBG17] - Clone mBG17, recombinant mouse monoclonal antibody targeting... Anti-SARS-CoV-2 Nucleocapsid Protein [mBG86] - Clone mBG86, recombinant mouse monoclonal antibody targeting...

...pMSCV PIG (Puro IRES GFP empty plasmid) MSCV For cloning and gene expression; select with puromycin or screen...

...Information During several different stages of molecular cloning, it is important to get a quick and accurate reading...

...also like... Polymerase Chain Reaction Plasmid Cloning by PCR Agarose Gel Electrophoresis Primer Design...

...vectors, DNA encoding zinc finger arrays should be cloned into plasmids also available through Addgene--the...

...Superfamily Research You may also like... Ras Pathway RAS Clone Collections Richard Kahn Lab Plasmids GTPase Plasmids...

...Cancer Drug Resistance Checkout the RAS Pathway Clone Collection ...

... you spent hours in the biosafety cabinet with cloning rings or following labor-intensive limiting dilution...

... alleles may be warranted (20-30% of all tagged clones were bi-allelic in our hands). Take-home message...

...be used with the recently developed Golden Gate cloning method to assemble “multiple DNA fragments in an...

...with fluorescent proteins to visualize and track clones of cells over time. Examples of useful tools for...

... based on factors such as selectable marker or cloning method. To isolate mammalian vectors, simply type...

...Digital Droplet PCR for AAV QuantificationPlasmid Cloning by PCR...

...contain an intron, preventing Cre expression when cloning in E. coli . This alteration enables the generation...deleting the floxed portion of a construct during the cloning process). VCre and SCre are alternative Cre recombinases...infection/transfection efficiency, selecting desired clones, and making transgenic mice. ID Plasmid/Article...

...Bacterial Transformation You may also like... Plasmid Cloning by Restriction Enzyme Digest Restriction Digest...