...decades to generate knock-out mice, but for various reasons it is not nearly as efficient in human stem cells...genetic background and do an apples-to-apples comparison of all those mutations. If you make induced pluripotent...backgrounds. It becomes more of an apples-to-oranges comparison. Addgene: Can you compare the TALEN kit to CRISPR...

...comparable experimental results. Using PiggyBac transposons, the Woltjen Lab compared different variants ...reprogramming discrepancies. The polycistronic piggyBac transposon vectors tested in this work are available at ...tool for use as a donor in FRET (fluorescence resonance energy transfer) and FLIM (Fluorescence Lifetime...1kb fragments are produced (see figure above). 3 Reasons The PSU Ladders Are Awesome Tools: They are comprised...

... Keown, a staff writer at the non-profit Fred Hutchinson Cancer Research Center. Susan Keown’s science...applied and I got it.” Science writing at Fred Hutchinson Cancer Research Center Work as a Science Writer...as you can to get your name on things.” Susan personally found this easier to do while still in school...

...ligase-mediated method, the more recently developed Gibson Assembly Cloning and Gateway® cloning technologies... toxic protein (CcdB) that acts as a DNA gyrase poison, locking up DNA gyrase with broken double stranded...killing by the F plasmid CcdB protein involves poisoning of DNA-topoisomerase II complexes.” J Mol Biol...

...Abrahrnsen L, Oesterlof B, Josephson S, Oestling M, Enfors SO, Persson I, Nilsson B and Uhlen M. Large-scale...

....34870.  Kaas GA, Zhong C, Eason DE, Ross DL, Vachhani RV, Ming G-L, King JR, Song H, Sweatt JD (2013) TET1...linked to disease has now become much like the 1980s song “Don’t Stop Believin’”; whenever you hear it (whether...

...spike protein, called HexoPro, was developed by Jason McLellan’s lab. This version of the spike protein... Lee AG, Liu Y, Chou C-W, Byrne PO, Hjorth CK, Johnson NV, Ludes-Meyers J, Nguyen AW, Park J, Wang N, ...Seaman MS, Schmidt AG, Chen B, Neuberg D, Bajic G, Harrison SC, Wesemann DR (2021) Memory B cell repertoire...

...Bradbury ARM, Trinklein ND, Thie H, Wilkinson IC, Tandon AK, Anderson S, Bladen CL, Jones B, Aldred SF, ...piece of music. It was tough to share or remix the songs, the CDs could get scratched over time, and it was...

...at chromosome 19q13.4 qtr. (AAVS1)). For these reasons, AAV is usually classified as a BSL-1 safety level...General viral handling safety   Containment and personal protection are key to lab safety and the same ...reduce the risk of an accident (the most common reason for lab accidents is not following safety protocols...

...to non-scientists and may make your perfectly reasonable paragraph unintelligible to a lay audience. ...HR on the judging panel. It’s important for this person to get a clear and concise explanation as to why...Writing Professional Facilitate Career Planning and Personal Development with an IDP Get Tips on Keeping Up...

... both NeuroMab and the Allen Brain Atlas. The comparison with two atlases enables us to see the reproducibility...literature suggest, providing an expectation and comparison on the antibody signal’s location. If a distinct... discuss the results together, explaining our reasoning to determine if the antibody is negative (poor...

... of tea, and you are more of an audio or visual person. Why don’t you record a podcast or put some effort...yourself as an educator for a short, animated TED-Ed lesson. 6) Network – Mingle with pros Where do you meet...Communication. In the US, UC Santa Cruz, MIT, UW Madison, and Boston University offer science communication...

...PMID: 14723844. 3. Atasoy D, Aponte Y, Su HH, and Sternson SM. 2008. A FLEX switch targets Channelrhodopsin...PubMed Central PMCID: PMC2593125. 4. Saunders A, Johnson CA, Sabatini BL. 2012. Novel recombinant adeno-...Wenbiao Chen Liquin Luo Massimo Scanziani Scott Sternson Roger Tsien Naoshige Uchida Ian Wickersham Hongkui...

...adolescence passed on a heritable trait to their grandsons that decreased their likelihood of death from ... Ong-Abdullah, Meilina, et al. "Loss of Karma transposon methylation underlies the mantled somaclonal .... 3. Kaati, Gunnar, Lars O. Bygren, and Soren Edvinsson. "Cardiovascular and diabetes mortality determined...

...slightly if you know a bit more about the other person. If you have a poster or talk at the conference...there parts of your elevator pitch where the other person seemed confused, bored, or particularly excited...after. For one conference I went to, I made it a personal goal to ask one question each day (this may be...

...., Polali, S., Anderson, E. D., Bell, A. M., Tzouanas, C. N., Avants, B. W., & Robinson, J. T. (2019).....4124.  Kattnig, D. R., Evans, E. W., Déjean, V., Dodson, C. A., Wallace, M. I., Mackenzie, S. R., Timmel...

...recommend using a Tris-based lysis buffer and sonication.  The obtained virions from cells and supernatant....2020.120 Lock, M., Alvira, M. R., Chen, S.-J., & Wilson, J. M. (2014). Absolute determination of single-stranded...L. H., Xiao, R., Lock, M., Lin, J., Korn, M., & Wilson, J. M. (2010). Efficient serotype-dependent release...

...efficiency. Figure used with permission from the Mark Dawson lab Re-transformation of the SPLINTR Libraries ...recombined backbones that may replicate faster. For this reason, if your library has inserts of different sizes... Hannah Dotson...

...is very tricky to use and fussy to optimize. One reason could be the instability of the supposedly alien...many may find this inconvenient. Another possible reason for the trickiness of NgAgo is the inability of... not snipping DNA, he tries random things (101 lessons on Spanish, C++, guitar, photography) and often...

...next thing. If I made a mistake, I evaluated what lessons I had learned – call it “troubleshooting.” Trying...I asked some colleagues to share valuable life lessons they have learned during their scientific training... them in a blog post on Born to Science: Life Lessons from Scientific Training More and more voices ...