...developed the plasmids, pPSU1 and pPSU2. When restriction digested with PstI or EcoRV, these plasmids generate...significantly cheaper than commercial ladders. Are you interested in making your own DNA ladder? You can find the...available from Addgene. Learn How to Analyze a Restriction Digest References 1. Henrici, R. C., Pecen, ... Miniprep Protocol Learn How to Analyze Your Restriction Digests Make Your Agarose Gel Other Resources...

...parts in a specific orientation using Type IIS restriction enzymes (Weber et al., 2011). It can be performed...units (TUs) used to express your favorite gene-of-interest, all the way to the complex multi-TU constructs...and tags makes EcoFlex a great option for those interested in recombinant protein purification and metabolic...looking to express high levels of their gene of interest in yeast should also take a look at the Yeast ...stacking, increasing production of a protein of interest, and improving the nutrient profiles within given...

...dabbles in science policy, public outreach, and restorative justice.  Follow her on twitter @sung_vanessa...age live in a state with at least two abortion restrictions that conflict with scientific evidence. We need...Campus.  He studies viruses including HIV and is interested in global health policy.  He is also chair of...Michigan, where programs like the Great Lakes Restoration Initiative are at risk of major cuts and where...science and positively reinforce their scientific interests. Our marches across the world have had an impact...

...wanted to measure expression of your protein of interest in a single cell? Or perhaps, you need to analyze...fluidics system they pass through a flow cell which restricts the size of the stream and forces the cells to...? However, fusion proteins tag your protein of interest with a fluorescent reporter on the N- or C-terminus... around the specific population that they are interested in and further analyze that subset of cells. .... An immunologist, for example, might be interested in the lymphocytes and “gate” that population for ...

...always been interested in the ways that I could change the world around me. My strong interest in the intersections... marketing internship at Addgene. Although my interests are very clear, I had no idea how to translate...would be a perfect way of exploring my different interests but also a way of challenging what I am used to...

...multi-color imaging experiments. Interested in epitope tags? Others may be interested in attaching epitope tags... fluorescent proteins (FPs) to a yeast gene of interest. These fluorescently tagged genes are expressed...tags to their genes of interest, allowing for easy capture and detection of proteins and complexes, without...

...heavy restrictions on what I could and couldn’t do as an intern. I expected that these restrictions would...choose what gene to focus on. The gene choice had restrictions. It couldn’t be a Covid gene, a toxin, or an...running on a time crunch and knew that if we were to restart the procedure we would not have enough time. During...

... students to network, find groups with common interests, and settle into university life. The fairs are...1996, Cambridge Biosoc’s goal has been to promote interest in the biological sciences amongst the student...meetups. The society hopes to help young scientists interested in this field excel and develop their career ...from, and mentor while pursuing your research interests. Scientific societies like those mentioned above...

...the first gRNA using restriction enzyme BbsI and the second gRNA using restriction enzyme BsaI. If you’... of gRNAs Golden Gate Assembly uses Type IIS restriction enzymes, which cleave outside of their recognition...These expression vectors each contain Type IIS restriction sites flanking the promoter-gRNA construct, but...oligonucleotides. These plasmids contain Type IIS restriction sites that flank the promoter-gRNA constructs...

...well-equipped. Many institutions are sincerely interested in connecting with the public in a meaningful...scientific background and bring with them established interests and areas of expertise. Perhaps the strongest ... disparate spheres of research. In my case, an interest in neuroscience was carried over and allowed to... new intellectual environment. Over time, this interest came to incorporate other facets which occupied...

...plasmid with your gene of interest, you would replace mCherry with your gene of interest. pBLADEONLY: This plasmid...a pBAD33-based plasmid expressing your gene of interest, all you have to do is co-transform pBLADEONLY...pBLADEONLY. That way, a previously cloned gene of interest, under the pBAD promoter, can be controlled with light...

...available. Aguilar is a basic cell biologist. He got interested in Lowe Syndrome initially because of studies...disease associated with the gene, it piqued their interest. They obtained cells from patients with the condition...of the Wistar Institute, says that the lab got interested in XP out of a desire to understand the early...diseases at the same time. The limitation of budget restrained us from costly functional analyses.  “We believe...

...recapitulate the natural biology of a cell type of interest better than immortalized lines derived from the...it’s much more difficult to introduce a gene of interest into primary cells, so most primary cell lines...cell penetrating peptide (CPP) to a protein of interest thereby giving it the capacity to enter cells....29(1):33-41. PubMed. Protein transduction: unrestricted delivery into all cells? Schwarze SR, Hruska...

...protein (BFP, GFP, or RFP) to the genomic loci of interest. Pairs of identical hairpins within one gRNA will...HaloTag with red fluorescent ligands to the loci of interest. Dual color detection In order to label two loci...sgRNA-Sirius-8XPP7) to recruit GFP to the loci of interest. To determine how the sgRNA-Sirius-8XPP7 and sgRNA-Sirius...that result in yellow fluorescence at the loci of interest, the team could visualize three loci and measure...

...standardized DNA constructs, but its use of Type IIS restriction enzymes results in scarless assembly. Finally...allowing fragments to be taken directly from restriction enzyme digests to DNA assembly. What makes it...containing regions of homology) cut from plasmids via restriction enzyme digestion, and used directly in DNA assembly...as easy as obtaining a plasmid with the gene of interest (search Addgene plasmids by gene here), designing...

...community by funding their travel to a conference of interest. In this post, we’re proud to announce our first...components - an enzyme that breaks down the HMO of interest into its component simple sugars (including lactose...controlled GFP. When the enzyme breaks down the HMO of interest, the lactose released activates expression of ...His-tagged ABC transporter known to bind to the HMO of interest. Fatima specifically proposes using this sensor...

...Relationships need nurturing. Perhaps you’ve met someone interesting at an event. Or, perhaps you have a colleague...or other information that may be helpful or of interest to them. Don’t send a long email. If you have ...with people by discussing non-work topics and interests. Offer help when you can, instead of just asking... with people you don’t like or who do not show interest in you. Network with the people that you want ...

...site of interest. A gRNA is used to direct the PolI3M-nCas9 complex to a DNA site of interest, which nCas9...introduce diversification by targeting a site of interest with dCas9 fused to the Activation Induced Cytidine...nicking Cas9. A guide directs Cas9 to a site of interest, where it nicks its target DNA (left). Cas9 dissociates...

...with antibodies specific to your protein(s) of interest. Once the complex is captured, it is treated so...digestion can be appropriate when your protein of interest is a low abundance transcription factor or cofactor...point you have (1) crosslinked your proteins of interest to any DNA they were bound to (2) fragmented the...antibodies to specifically select for proteins of interest (4) isolated your antibody:protein:DNA complexes...

... spectrophotometer Restriction Digests Cut and analyze plasmid DNA with restriction enzymes Agarose Gel...pooled-plasmid libraries Diagnostic Restriction Digest Verify a plasmid using restriction enzymes Watch the Video!...new window) Link to Video Restriction Cloning Assemble plasmids by restriction digest and ligation Cloning...Cloning by PCR Generate restriction sites by PCR Modification by Annealed Oligo Cloning Add a short stretch... Cloning (LIC) Scarless cloning with Type II restriction enzymes and T4 polymerase pLKO.1 - TRC Cloning...