...consider the donor first where labeling specificity is most important. Donor labeling and the need for specificity...changes due to this bulky insertion are inevitable. Most FP fusions for FRET studies are at the N- or C-terminus...is required and thus far, this method has proven most effective only in a few cell types (E. coli and ... in chemical warfare [Vilensky & Redman, 2003]). Most importantly, these compounds cross cell membranes...

...various tools and components necessary to run it? And, most importantly, what are the methodological and experimental...one: Deciding how you want to use optogenetics The most important question to consider is what exactly do...neuronal promoters such as synapsin or camKIIα drive most of the constructs. As an alternative, some constructs...of interest (as shown in the figure below). Since most of the precision of optogenetics is dependent on...

...year of first reagent sent, year with most reagents sent, and the most recent full calendar year When the... information can provide valuable insight to the most requested plasmid, the number of requested plasmids...

...Editor's Choice" series. Each month, I'll summarize the most popular post of the month and point out one or more...excitement surrounding anything to do with CRISPR, our most popular post in July (with over 700 views) was an...and similar on-target editing when compared to the most commonly used Cas9 variant, SpCas9. These properties...

...Developmental Studies Hybridoma Bank. Antibodies are the most widely used class of protein-binding reagents. In...monoclonal antibodies obtained from serum.  Our most popular monoclonal antibodies include those directed...More Blog Posts about Scientific Sharing: The 10 Most Distributed Plasmid Technologies in Addgene's First...

... of all citations in 1995 to 47% in 2013 – yes, almost half.   The effect of online access on scientific...interesting aside, Nature recently reported that the most cited work in history is a 1951 description of an...Prize-winning research typically weren’t among the most cited. And, as scientific discourse has increasingly...

...Marketing Coordinator Networking “Many, perhaps most of the most interesting jobs are never advertised. You...favorite career tip is, or what has helped them the most. Take a look below! In the lab “Have two projects...

... Protein purification can be one of the most stressful lab activities. Working with proteins requires...proteins are overexpressed from a plasmid construct, most often in special E. coli strains designed for protein...few different vectors to see which gives you the most functional protein. The pCri system, recently deposited...

...coding, labels, and post-its to make finding your most used protocols & references easy to find It’s happened...know where to start your search. Then identify the most frequently used pages in your notebooks and go wild..., or references that your labmates ask to borrow most frequently. Copy these into an electronic file sharing...

... 50 base pairs of sequence homologous to the chromosomal site of insertion. The linear PCR product is ...associated with plasmid-based overexpression. Tim Formosa, at the University of Utah, has built a complete...terminator-(selection marker). Additionally, Dr. Formosa has deposited six plasmids with a multiple cloning...

...my interest was renewed in part due to FOMO  but mostly because of this article: “A systematic identification...2017. 1. Among scientists, historians have the most Twitter users. Biologists rank #8. Figure 1: Highly...than other disciplines. The majority of tweets for most disciplines contain scientific domains between 0...

...Overview of the parts of CRISPR. The bacterial chromosome encodes a tracrRNA (in some systems including...are called Cas, short for “CRISPR-associated.” The most famous of these proteins is Cas9 nuclease, but there...DNA CRISPR array: The section of the bacterial chromosome that stores a genetic record of previously encountered...

... Unfortunately, unless they’ve worked in a lab, most people don’t get to experience this very human side...the stop motion format for our videos as it is the most approachable for LEGOs. Stop motion consists of ...takes an entire week of our free time. After taking almost a thousand pictures, my husband Enzo will spend...

... a scientist. It is shocking how little pre-work most PhD students and postdocs do to ensure the advisors...inhibited in sharing concerns. Ask alumni of the lab. Most labs have websites listing alumi and it is easy ...ResearchGate and LinkedIn to find past lab members. Most scientists love to talk about their past lab experiences...

...non-academic jobs as “alternative” anymore.   Since most scientists start out in academia, it is especially...waiting Work is concentrated to business hours (mostly), but can often achieve more structured time management...and what parts of being a scientist you enjoy the most. Having a better idea of where you want to go will...

...year were synthetic. They also found out that the most common expression system within the Addgene repository...of unique gene sequences is Proteobacteria - the most frequent transfer of genes is from Proteobacteria...biology page Search for a plasmid Find Addgene's most popular plasmids ...

... development as your mentor. How do you make the most of this new relationship? Consider adding formality... a mentoring relationship really productive, the most important step you can take is to set goals to work...teaching to help a Mentee reach goals. One of the most important contributions made by a Mentor is to help...

...fluorescent proteins (FPs) have become one of the most indispensable tools in modern biological research...probe (be it an organic dye or FP) is one of the most important steps in the design of an imaging experiment...soon as possible after submission. Using FPbase The most obvious use case for FPbase is finding information...

... plasmids. These libraries are some of Addgene’s most exciting and versatile offerings! We recently re-amplified...protocol, but the overall workflow is similar for most libraries. Here are the key points you’ll need to...Figure 3A). Addgene finds that this representation is most easily understood visually. As an example, the curves...

...cell differentiation fate to tumor suppression (and most everything in between), understanding how, when,...selections In its current format, the MORF library is most immediately useful for TF library screens. Previously... More about Lentivirus Figure out How to Get the Most from Your Lentiviral Transduction Additional Resources...