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  1. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...isolated which target and cleave RNA through the same mechanism as traditional spCas9. If you are looking...Cas13 and has been proposed to be useful for the same applications (RNA knockdown, RNA editing, etc.)....

  2. Summer SciComm: Preprints

    Type
    Blog Post
    ...communication. Researchers often post a preprint at the same time as, or slightly before, submitting the manuscript...plasmids that were used in a preprint provides all the same benefits as sharing those plasmids alongside a journal...

  3. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...non-physiological RNA-protein interactions due to sample processing. Require prior knowledge that the protein...control). BioID2 had no detectable signal at the same time point. E. coli’s BirA* was tested at its optimum...

  5. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...& Walton et al). Additionally, SpG Cas9 with the same PAM preference of NG was isolated by the Kleinstiver...several bps away from a desired site does not have the same devastating impact on efficiency as 10-15 bps away...

  6. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ... viruses are more dangerous than others, and the same holds true for types of viruses generated in the...personal protection are key to lab safety and the same principles apply to viral handling. Gloves are always...

  7. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...between the sites. If a DNA sequence is floxed in the same orientation, the SSR will excise the DNA fragment...reporter with one loxP site and one lox511 site in the same orientation, and placing the additional loxP and...

  8. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...contact Addgene. We may require that you send your samples to Addgene pre-transformed in this strain. ...with instructions on how to prepare and ship the samples to Addgene and contact your technology transfer...

  12. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...necessary and sufficient for retrograde transmission (Etessami et al., 2000, Wickersham et al., 2007). Thus, ...Mar 19;110(12):E1152-61 Pubmed PMID 23487772. 3. Etessami R, Conzelmann KK, Fadai-Ghotbi B, Natelson B, ...

  14. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...answers uninteresting, the panel members all say the same thing and one panel member dominates the discussion... for an intimate event, put the panelists on the same level as the audience. In my experience the best...
Showing: 261 - 280 of 581 results