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We narrowed to 601 results for: sam

Showing: 261 - 280 of 601 results
  1. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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    Blog Post
    ... the ccdB aspect, Gateway takes advantage of the same principle that cells will not propagate while expressing.... While these two strains ultimately perform the same function, there is some evidence that ccdB Survival...
  2. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...necessary and sufficient for retrograde transmission (Etessami et al., 2000, Wickersham et al., 2007). Thus, ...Mar 19;110(12):E1152-61 Pubmed PMID 23487772. 3. Etessami R, Conzelmann KK, Fadai-Ghotbi B, Natelson B, ...
  3. Viral Vectors 101: Virus Safety

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    Blog Post
    ... viruses are more dangerous than others, and the same holds true for types of viruses generated in the...personal protection are key to lab safety and the same principles apply to viral handling. Gloves are always...
  4. Progress Towards a PAM-Free CRISPR

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    Blog Post
    ...& Walton et al). Additionally, SpG Cas9 with the same PAM preference of NG was isolated by the Kleinstiver...several bps away from a desired site does not have the same devastating impact on efficiency as 10-15 bps away...
  5. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...contact Addgene. We may require that you send your samples to Addgene pre-transformed in this strain. ...with instructions on how to prepare and ship the samples to Addgene and contact your technology transfer...
  6. Plasmids 101: FLEx Vectors

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    Blog Post
    ...between the sites. If a DNA sequence is floxed in the same orientation, the SSR will excise the DNA fragment...reporter with one loxP site and one lox511 site in the same orientation, and placing the additional loxP and...
  7. How-to: 5 Steps to a Great Panel Discussion

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    Blog Post
    ...answers uninteresting, the panel members all say the same thing and one panel member dominates the discussion... for an intimate event, put the panelists on the same level as the audience. In my experience the best...
  8. The AAVantages of AAV in CRISPR Screens

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    Blog Post
    ...vivo. The general process of these screens is the same: Deliver CRISPR library to cells of interest. Select...Low, S. H., François, A., Suárez-Amarán, L., & Samulski, R. J. (2022). AAV vectors: The Rubik’s cube of...
  9. Production of Virus in Insect Versus Mammalian Cells

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    Blog Post
    ..., so the amount of cloning required is about the same between insect and mammalian systems. Baculovirus...P1 virus, so that if you ever need to remake the same virus, you can skip the first round of production...
  10. Viral Vectors 101: Producing Your rAAV

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    Blog Post
    ...known titer on every qPCR run. Note that the rAAV sample has to be digested with DNase before undergoing...Grimm, D., Kern, A., Pawlita, M., Ferrari, F., Samulski, R., & Kleinschmidt, J. (1999). Titration of AAV...
  11. Pooled Library Amplifications

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    Blog Post
    ...library from Addgene, they’ll need to amplify the sample for further downstream applications. As individual... all of the bacteria can grow and compete in the same container, making it easier for a smaller, recombined...
Showing: 261 - 280 of 601 results