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We narrowed to 611 results for: sam

Showing: 261 - 280 of 611 results

  1. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...hypodermis and gut cells of C. elegans causes the same phenotype. Activation of the Raf-MEK-ERK signaling...signaling pathway with psRaf1 or psMEK1 in these same cells by 24-48 hours exposure to blue light also induced...

  3. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...non-physiological RNA-protein interactions due to sample processing. Require prior knowledge that the protein...control). BioID2 had no detectable signal at the same time point. E. coli’s BirA* was tested at its optimum...

  4. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ... the ccdB aspect, Gateway takes advantage of the same principle that cells will not propagate while expressing.... While these two strains ultimately perform the same function, there is some evidence that ccdB Survival...

  5. Summer SciComm: Preprints

    Type
    Blog Post
    ...communication. Researchers often post a preprint at the same time as, or before, submitting the manuscript to...plasmids that were used in a preprint provides all the same benefits as sharing those plasmids alongside a journal...

  6. Addgene: The First Twenty Years

    Type
    Blog Post
    ...Because we have a resource like Addgene to all get the same virus.” — Grad student visiting Addgene’s booth ...quality reagents, and we realized we could use the same principles to provide recombinant antibodies, another...

  7. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...episomal and does not divide and replicate in the same way the host DNA does. Because of this, fast dividing...McCarty, D. M., Paul E. Monahan, and Richard J. Samulski. "Self-complementary recombinant adeno-associated...

  8. The time and cost required to make a plasmid

    Type
    Blog Post
    ...overall inflation of USD has gone up 19.8% in the same time period. For ease of calculations*, let’s split...inflation at… roughly 34.9%, which means that the same plasmid would cost $155.25 in reagents and consumables...

  9. All in a Twist: dsRNA

    Type
    Blog Post
    ...systems can lead to local dsRNA production. In the same vein, genotoxic stress, such as chemotherapy treatment...dsRNA finds its way outside of the mitochondria, the same inflammatory response can activate. These types ...

  10. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...isolated which target and cleave RNA through the same mechanism as traditional spCas9. If you are looking...Cas13 and has been proposed to be useful for the same applications (RNA knockdown, RNA editing, etc.)....

  14. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...necessary and sufficient for retrograde transmission (Etessami et al., 2000, Wickersham et al., 2007). Thus, ...Mar 19;110(12):E1152-61 Pubmed PMID 23487772. 3. Etessami R, Conzelmann KK, Fadai-Ghotbi B, Natelson B, ...

  16. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...contact Addgene. We may require that you send your samples to Addgene pre-transformed in this strain. ...with instructions on how to prepare and ship the samples to Addgene and contact your technology transfer...

  18. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...& Walton et al). Additionally, SpG Cas9 with the same PAM preference of NG was isolated by the Kleinstiver...several bps away from a desired site does not have the same devastating impact on efficiency as 10-15 bps away...

  19. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ... viruses are more dangerous than others, and the same holds true for types of viruses generated in the...personal protection are key to lab safety and the same principles apply to viral handling. Gloves are always...
Showing: 261 - 280 of 611 results