...all of our CRISPR blog posts Download the CRISPR 101 eBook Read our genome engineering blog posts Resources...Addgene.org Read our CRISPR guide Find CRISPR plasmids by function Check out our genome engineering guide...

...Improvement of pCVD442, a suicide plasmid for gene allele exchange in bacteria." Plasmid 51.3 (2004): 246-255. PubMed...Additional resources on the Addgene blog Read our Plasmids 101 blog posts Find our CRISPR blog posts Learn ...exogenous enzymes, require multiple transformation and plasmid curing steps, and can generate off-target mutations...a mutated allele introduced from a conjugative plasmid replaces the chromosomal copy. This is a versatile... These two steps are (Figure 1): The initial plasmid integration step (the so-called “single crossover... an allelic exchange experiment? Find the pTOX plasmids here! Continued development of new allelic exchange...recombineering Resources on Addgene.org Find plasmids for allelic exchange Read our molecular biology...

...and Myers Tagging Plasmids Protein Species Tag Donor Plasmid gRNA plasmid gRNA plasmid FLAG pFETCh_Donor... as the plasmids and protocols needed for using them, are described below. Read our CRISPR 101 post for.... Multiple gRNA plasmids may be used. The HDR (homology directed repair) donor plasmid with homology arms...individual tagging event. The kit contains 26 plasmids; 21 of the plasmids are for use in SapTrap reactions for...the remaining 5 plasmids include Cre and FLP expression vectors, a general cloning plasmid, and a prebuilt...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR plasmids for endogenous tagging of your gene of interest. CRISPR...

...aptamer downstream of a known promoter. Addgene’s Plasmids 101: Aptamer Fluorophores describes the enrichment...Browse fluorescent protein plasmids and resources See all Jaffrey lab plasmids ... cellular processes by transforming cells with plasmids encoding aptamer-tagged RNA and later introducing...chemical biology 9.10 (2014): 2412-2420. PubMed PMID: 25101481. Filonov, Grigory S., et al. "Broccoli: rapid ...

... CRISPR User) The Challenges of Cell Culture Plasmids 101: Mammalian Vectors Resources on Addgene.org...Our Protocols Pages Browse Fluorescent Protein Plasmids Find Mammalian Expression Vectors ...of sterile truncated pipet tips (USA Scientific #1011-8410) is also placed in the hood. Finally, a sterile...

...Agarose Method Plasmids 101: Mammalian Vectors Resources on Addgene.org Find mammalian plasmids for your ...

...of Cre-lox. Check out our previous blog post, Plasmids 101: Cre-lox, if you need a quick primer on how ...research? Check out Brainbow plasmids available from Addgene! Find Brainbow Plasmids @Addgene: Brainbow 1.0...1.0, 1.1, 2.1 plasmids Brainbow 3.0, 3.1, 3.2 plasmids References: Transgenic strategies for combinatorial...

...Gating in Flow Cytometry Antibodies 101: Flow Cytometry Antibodies 101: Validation Resources on addgene.... (tagged or untagged) protein When expression plasmids are available, or the necessary expertise to clone...involves the transient transfection of expression plasmids in a given cell line. For flow cytometry, it is...

... Find CRISPR Plasmids for use in Mammals Check out Our CRISPR Guide Pages Find Plasmids for Mouse Targeting...the target site, and a donor oligonucleotide or plasmid as the repair material if needed (for knock in ..., the gRNA, Cas9, and donor oligonucleotide or plasmid components are brought together and microinjected...provided (in the form of an oligonucleotide or plasmid), precise changes can be made in the genome via...mice carrying the intended mutation. With a donor plasmid, the outcome is less predictable. In our best case.../srep32565 Resources on the Addgene Blog CRISPR 101: Mammalian Expression Systems and Delivery Methods...Viral Vector. iScience 9:286–297 . https://doi.org/10.1016/j.isci.2018.10.030 Qin W, Kutny PM, Maser RS, Dion...

...the CRISPR 101 eBook Resources on Addgene.org Read the CRISPR guide Find CRISPR plasmids by function...function Browse our entire plasmid collection ... Cell, 184(22), 5635-5652.e29. https://doi.org/10.1016/j.cell.2021.09.018 Nelson, J. W., Randolph, P. ...Target DNA. Cell, 156(5), 935–949. https://doi.org/10.1016/j.cell.2014.02.001 Yan, J., Oyler-Castrillo, P....

...Vectors 101: Optogenetics Additional resources on Addgene.org Optogenetics Guide Optogenetics Plasmids References...module, which are also available as lentiviral plasmids if you want to make a stable cell line. This design...developing embryo is accessible for injection of plasmids and other materials.    Figure 3: Optogenetic...Guide or browse our collections of optogenetics plasmids – and keep an eye out for future updates to the.... PLoS Genetics, 19(5), e1010765. https://doi.org/10.1371/journal.pgen.1010765.  Berlew, E. E., Yamada...Biology, 32(21), 4660-4674.e6. https://doi.org/10.1016/j.cub.2022.09.010.  Ridley, A. J. (2016). Rho GTPase... in Cell Biology, 36, 103–112. https://doi.org/10.1016/j.ceb.2015.08.005.  Tei, R., Bagde, S. R., Fromme...

...lentiviral plasmids Additional resources on the Addgene Blog  How to design your CRISPR gRNA CRISPR 101: Ribonucleoprotein... Cell 81, 7, 1553-1565 (2021). https://doi.org/10.1016/j.molcel.2021.02.007 Moroz-Omori, E., Satyapertiwi...

...Additional resources on the Addgene blog Find our Plasmids 101 blog posts Read blog posts about using Cre-lox...

... about FLEx vectors Download our Plasmids 101 eBook Read our plasmids blog posts Resources on the Addgene...molecular biology reference Find Cre-lox plasmids at Addgene Browse plasmids at Addgene ...stimuli in the mesolimbic dopamine system." Neuron 101.1 (2019): 133-151. PubMed PMID: 30503173. PubMed Central...the nervous system: a clinical reality." Neuron 101.5 (2019): 839-862. PubMed PMID: 30844402. Juarez, ...

...Additional resources on the Addgene blog Read our CRISPR 101 blog posts Learn about some considerations when designing...software Resources on Addgene.org Find CRISPR plasmids and resources Download the CRISPR eBook Read our...

...CRISPR 101 blog series Resources on Addene.org Read Addgene's CRISPR Guide Find CRISPR plasmids for your...Expression. Cell 152:1173–1183 . https://doi.org/10.1016/j.cell.2013.02.022 Sajwan S, Mannervik M (2019)...Fluorescence Imaging. Cell 159:635–646 . https://doi.org/10.1016/j.cell.2014.09.039 Zhou H, Liu J, Zhou C, Gao N...

...comprehensive data sheets on many FP variants Plasmids 101: Green Fluorescent Protein (GFP) Three Review...

...Blog Read Our CRISPR 101 Posts Read Our Plasmids 101 Posts Read All of Plasmid Technology Posts Resources...amazing plasmid technologies developed by our community of depositors. With over 40,000 plasmids avaliable...amazing new plasmid tools that have come through our doors in 2015. Do you have a favorite new plasmid tool ...system plasmids are available through Addgene and are already very popular. Use these plasmids to increase... can visualize: Cell Structure Plasmids: Cell Structure Plasmids: ER mCherry-Sec61 beta BFP-Sec61...luciferase reporter gene plasmid pGL3 pGL3 luciferase reporter gene plasmids have been used extensively...find all of our Hot Plasmid articles on our website. We update the our Hot Plasmids articles every quarter...

...! More resources on the Addgene blog Antibodies 101: ELISAs The Four ELISAs and When to Use Them A Control...Antibody collection Addgene's Recombinant Antibody Plasmids collection  ...

...Use PASTE can be delivered via a single dose of plasmids, works in non-dividing and primary cells, and ...Precision and Flexibility to CRISPR Engineering CRISPR 101: RNA Editing With Cas13  CRISPR Cheat Sheet Resources...bioRxiv 2021. 11.01.466786; doi: https://doi.org/10.1101/2021.11.01.466786 Additional Resources on the Addgene...