Skip to main content
Addgene
Showing: 261 - 280 of 525 results

  2. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ... using droplet digital PCR Droplet digital PCR involves partitioning a PCR reaction mixture into approximately... Witte, Anna Kristina, et al. "A systematic investigation of parameters influencing droplet rain in the...

  4. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...ownership of their work and become more actively involved in the learning process. As trainees become more... and effort and/or perceived professional risk involved in setting up a CURE, especially if it’s a new...

  6. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ... in using the 3rd-generation system is that it involves transfecting four different plasmids into the ...quickly check that a construct is functional before investing time and resources into making a stable cell line...

  7. Lab to Office Culture Shock

    Type
    Blog Post
    ...culture shock? Working in an academic research lab involves a few key features that can be difficult to replicate...sometimes wish for a stretch of time that doesn’t involve critical thinking. Former Addgenie Jessica Welch...

  9. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...gene commonly associated with CRISPR arrays and involved in spacer acquisition following infection, as ... we appreciate the opportunity to share these invaluable reagents with the scientific community. Note...

  10. How to Write a Scientific Review Article

    Type
    Blog Post
    ... Perhaps you, a colleague, or your adviser was invited to write on a particular topic. Alternatively, ...review articles Whether submitting a review by invitation or by your own accord,  once you have these rules...

  11. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...resources, time, and experimental needs. Since its invention in the late 1990s, Gateway cloning technology ...technology The Gateway cloning method, developed by Invitrogen, is an in vitro version of the integration and...

  12. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...resistance testing is requested clinically, this involves growing a standardized amount of the bacteria ...focus on creating new antibiotic therapies, and investigate more effective drug targets (ones that are less...

  13. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...in a competitive ELISA the reporter signal is inversely proportional to the level of antigen in the sample...in a competitive ELISA the signal intensity is inversely proportional to the amount of antigen in the sample...

  14. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...researcher see which of the multiplexed sgRNA was involved in a particular edit, and which type of edit was...open source and free for all non-profit uses. We invite you to try ICE today and let us know how it works...

  16. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...delivered through transfection of naked DNA. Other investigators who had been working to develop high efficiency..., which is particularly well suited for strand invasion, a key step in the homologous recombination pathway...

  18. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ... a suicide switch in cyanobacteria, synthetic inverters in E. coli, and biosensors in E. coli: Čelešnik...017129. Hsia, Justin, et al. "Modular Synthetic Inverters from Zinc Finger Proteins and Small RNAs." PloS...

  19. Evolution of Lab Techniques

    Type
    Blog Post
    ...real-time PCR Southern blotting, named for its inventor Edward M. Southern (1), can be used to detect ...commonplace, researchers used restriction mapping to investigate DNA sequence content by comparing the fragments...

  20. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...sample type. Sample Preparation This step can involve multiple smaller steps that all help to ensure ... Similar to Sample Preparation, this step can involve multiple smaller steps, but the aim is to preserve...
Showing: 261 - 280 of 525 results