...technology or as a therapeutic.  A (very) brief history GCE came onto the synthetic biology scene in 1989.... While the codon used is often one of the three stop codons ­– most commonly the UAG codon – in some ... V. W. (2021). Genetic Code Expansion: A Brief History and Perspective. Biochemistry, 60(46), 3455–3469...

...project, I read the book “Every Other Thursday: Stories and Strategies from Successful Women Scientists... group to be intrinsic to their success. Their stories made it clear to me that, executed correctly, the...renowned Healthcare Businesswomen’s Association (HBA) Boston Chapter and the Massachusetts Association for Women...

...cells. LITE Two-hybrid system integrating the customizable TALE DNA-binding domain with cryptochrome 2 ...

...targeted the following antigens: GFP, RFP,  the histone protein H2A2B, and the actin-binding protein gelsolin...however, compatible with most multi-labeling immunohistochemical protocols where either a directly labeled...RANbodies) as versatile, small, sensitive immunohistochemical reagents. PNAS. PubMed PMID: 29440485. Additional...

... by guest bloggers Joachim Goedhart and Marieke Mastop from the Section of Molecular Cytology and Van ...our guest bloggers Joachim Goedhart and Marieke Mastop! Joachim Goedhart is an assistant professor at ...follow him on twitter: @joachimgoedhart.   Marieke Mastop is a PhD candidate at the Section of Molecular ...

...faculty (at time of writing, Brigham And Women's in Boston also just announced that they were reversing their...does not appear to hold. There are institutions in Boston - Massachusetts General Hospital, Mass Eye and ...that are not raising salaries when all others in Boston (some of them literally across the street from ...

...well received, and then when the team started brainstorming ideas for the next workshop, they realized that...to practice and teach reproducibility An early milestone for the group was the “reproducibility for everyone... Schwessinger for helpful conversations on the history of the group, lessons learned, and to Sonali Roy...

...Segall-Shapiro, Thomas H., Eduardo D. Sontag, and Christopher A. Voigt. "Engineered promoters enable constant...2. Kittleson, Joshua T., Sherine Cheung, and JChristopher Anderson. "Rapid optimization of gene dosage...Synthetic Biology: A Conversation with MIT’s Christopher Voigt Resources on Addgene.org See the synthetic...

...indicated below and pool-clone them into the pAWp28 storage vector (Figure 1): Forward oligo: 5’- CACCGNNNNNNNNNNNNNNNNNNNGTTTGGGTCTTCGAGAAGACCTATTCXXXXXXXXC...bp barcode, respectively. Once cloned into the storage vector, the pooled, barcoded gRNA library is now...backbone pAWp12 (Figure 2). Cloning from the pAWp28 storage vector to the pAWp12 lentiviral vector should retain...

...six figures. Averaging ten panels each. (For this story, it is important to know that the student presenting... it. When my mind went blank, my options were to stop talking until I could present something coherent... something as simple as telling yourself, “I can stop and check my notes if I need to,” or “It is okay...

...CRISPR editing in human embryos has been met with a storm of media coverage. But the paper published August... studies, the edited embryos proceeded to the blastocyst stage at similar rates to untreated controls....researchers were able to examine each individual blastomere to determine its genotype. However, this detailed...

...part of the recognition sequence, they can be customized to direct assembly of DNA fragments. When designed...utilize the BsaI and BsmBI Type IIS sites such that custom TAL arrays could be constructed quickly and efficiently...Nov 5. PubMed. Efficient design and assembly of custom TALEN and other TAL effector-based constructs for...

...experiences. Good coaches will have loads of success stories and plenty of people to vouch for them. Trust... of scientists here) I know many coaches in the Boston area. Sarah Cardozo Duncan is a proven coach who...has an outstanding blog and, while her PhD is in History, I think she would be a good match as a coach for...

...When this interaction occurs, BirA* activity is restored, and nearby proteins are labeled with biotin. ...but labeling ceases when these two bait proteins stop interacting. This feature is particularly useful...

... In the US, UC Santa Cruz, MIT, UW Madison, and Boston University offer science communication programs... and money to a full-time graduate course, UWE Bristol offers an intensive 4-day Masterclass in science...or upload your data to Zooniverse. The Natural History Museum has downloadable resources to enable you...

...science, dating back several hundred years, with historical ties to psychology and philosophy (the mind-body...in a certain type of behavior (for a review see Stoica et al., 2013). Vector delivery may also be achieved...service for their behavioral studies. References Stoica L, Ahmed SS, Gao G, Sena-Esteves M. Gene transfer...

...your target protein in each well. The reaction is stopped by the addition of hydrochloric acid (for more ...(TMB). The addition of hydrochloric acid, which stops the reaction, turns the solution yellow. The absorbance...serial dilution of your experimental sample, and/or stopping the HRP-TMB reaction more quickly. Just make sure...

...born before 1985, you might remember going to the store and buying CDs when you wanted to hear a piece of...technology makes it easy for scientists to share, remix, store, and track these essential components of the scientific...useful in perpetuity.  Second, plasmids are easy to store and share, making recombinant antibodies the most...

...plasmids at Addgene! Knock-in: Introducing tags or stop signals Once Cas9 generates double-strand breaks...very long ncRNAs is impractical. Instead, tags or stop elements can be inserted to disrupt the transcription... adjacent genes are complex and not yet fully understood, so it is important to carefully select the target...

...so many scientists who were potential Addgene requestors and depositors. China is in one of the top 10...good job of seeing tRNAs because they contain “hard stop” mutations. New techniques used by the Lowe lab ...can view public circRNA datasets and download the custom python scripts needed to discover circRNAs in RNA-seq...