Plasmids 101: 5 factors to help you choose the right cloning method
Type
Blog Post
... experiments are also generally comparable so the best way to compare costs is to look at the prices of...In today’s post, we will focus on a high-level comparison of popular cloning methodologies, with a special...least 50 cloning reactions. The ligase cost is comparable to that of the restriction enzymes and overall... in a price of a few dollars per reaction.
By comparison, Gibson assembly can cost over $15.00 per reaction...more streamlined, because they will come with comprehensive and tested protocols, as well as premixed and..., like Gibson or SLIC, since one can pool the components and define their final order through sequence...Golden Gate assembly allows one to simultaneously complete an ordered set of scarless ligations following...