...Special Collections). Arguably, oncology has benefited most as cancer cells are amenable to culture in vitro...about since 1966 when Stanley Gartler reported that most of the 18 cell lines he had studied (the majority...lines suffer from contamination with HeLa being the most frequent contaminant, closely followed by T24 bladder...their original description. The oldest, largest, and most diverse of these repositories is the American Type...

...essential. In this article, we’ll go through some of the most important considerations to make when planning and... tissue sample where they bind to their targets, most commonly proteins. You can then use fluorescent ...choosing a primary, so we’ll start there. Some of the most important considerations are to make sure your secondary...technique can provide you not only answers to your most pressing research questions, but also stunning images...

... even great, speaking skills can be attained by almost anyone with enough practice. I always take a poll...a lot of data.  I can’t say this enough…more is almost never better. 10. Be enthusiastic You don’t have...possible, practice enough so that you can deliver most of your talk without notes. Don’t be hard on yourself... “and who has deposited to the repository?” When most or all of the hands go down it is a great segue ...

... be treated with “one-size-fits-most” NHEJ-mediated editing, but most other diseases will require HDR-...Collections Pages Duchenne muscular dystrophy: The most common inherited disease The genetic disease targeted...editing used in the exon skipping approaches here. In most genetic diseases, the mutational landscape is broad...

...composed of an n-terminal DNA recognition domain and a mostly c-terminal nuclease domain. Feng Zhang's group ...untranslated in the nucleus, confirming the hypothesis that most mRNAs are not translated until they are exported...mammalian cells. Multicolor CRISPR labeling chromosomal loci in genome Thoru Pederson’s lab at the University...dynamic interactions between intra- and inter-chromosomal domains. The authors anticipate that this tool..., genome rearrangements and cancer-associated chromosome shattering. This method is based on the recognition...intra-nuclear distance between loci on different chromosomes. The advantage of having this kind of system ...can be difficult to compare their performance, as most translation initiation sequences are optimised for...

...designs if we do so. The most extreme version of this (and possibly the most effective if it were more... a result that confirms their hypotheses even if most other scientists do not. These scientists with positive...from the journal’s perspective, probably not what most readers want to read. As Susanna Bachle points out...

...SpCas9, with a PAM preference of NGG, is still the most widely-used Cas enzyme, the development of SaCas9... knockout via CRISPR cutting. For CRISPRa, it is most-efficacious to target a ~100nt window upstream of... a ~100nt window downstream of the TSS gives the most activity. Thus, a given gene will only have a dozen...CAGE-seq to directly capture the mRNA cap, provides the most accurate mapping (Radzisheuskaya et al., 2016). ...

...Broadly useful controls Positive and negative controls Most biological experiments require positive and negative...the experimental group.     Figure 1: A, Most biological experiments will benefit from positive...that must be considered (Burry 2011). Perhaps the most critical are those that confirm primary antibody...example at sites of inflammation. This tends to be most problematic when staining cells or tissues that ...

...essential to understand them in order to design the most specific assays possible. In this blog, we will ...tools generating a prediction for off-target levels, most of them will also give you a specific list of the... Graphic courtesy of Maya Kostman. The most commonly used Cas, spCas9, has gone through multiple...exact gRNA matches (Rabinowitz and Offen). Of note, most of these optimized Cas enzymes prevent cutting at...

...generation Tet-Off vectors (Tet-Advanced)” are the most recent variants of the Tet-Off system.  For more...post contains only a brief overview of some of the most common repressible promoter systems, but there are... PMC3574576. Lai, Sen-Li & Tzumin Lee. “Genetic mosaic with dual binary transcription systems in Drosophila... for transgene expression, lineage tracing, and mosaic analysis.” Cell 141(3) (2010): 536-48. PubMed PMID...

...recombinases. How might one read the state of an RSM? The most straightforward way to read the state of an RSM ...disadvantages. Sanger sequencing is simple and the most accurate method as it directly reads the state of...that we defined in our paper. In our opinion, the most convenient strategy is the fluorescent reporter ...output plasmid (single copy, bacterial artificial chromosome backbone) contains the register. Depending on...

... humanity were the two most popular topics that sparked the imagination of most of the authors during ...engineering in pop culture SciFi is one of the most imaginative and most important genres of literary fiction. ...

...but I didn’t know what level of expression was the most efficient for gene editing. A virus encoding GFP... practice you would select the Cas9 line that’s “most efficient at editing” to introduce the gRNA, but...regions of the gene with multiple gRNAs, but the most important is that you could avoid potential off ...sites targeted by my specific gRNAs (Figure 4). The most difficult part of the assay was designing primers...

...different DNA sequences or target sites. Integration of most transposable elements (TEs) results in the duplication...they do not use an RNA intermediary when they move. Most Class II transposons have a non-replicative “cut...different types of transposons, DNA transposons are most commonly used in the laboratory for genome manipulation...for both delivery methods, but since TEs insert mostly in intergenic regions, gene disruption is less ...

...self-labeling protein tags. The basics These tags most often consist of an enzyme that has been mutated...variants run up to HaloTag11. HaloTag is now the most popular self-labeling tag in Addgene’s collection...aren’t going to work in certain applications — the most common limitation is that certain probes are not...Jing & Cornish, 2011). HaloTag and SNAP-tag are the most popular self-labeling enzymes in Addgene’s collection...

...without a high number of samples (~5). Tukey’s test – Most conservative test. Higher false negatives, lower... false positives. Duncan’s multiple range test – Most powerful. Low false negatives, high false positives...detailed in this article. These are just some of the most common parametric comparison tests along with some...tool belt. To analyze your hard-earned data in the most effective and accurate way possible it’s important...

... you officially call it a “project team” or not, most scientific collaborations fall within this category...-game here, unless we manage to make Addgene the most environmentally friendly organization on the planet... Kennedy once famously said “I think this is the most extraordinary collection of talent, of human knowledge...don’t understand to this day, my post-doc adviser almost never had lab meetings. In my nearly 4 years in...

...that is more neutral, causing PA-GFP to display almost no fluorescence when illuminated with 488nm light...emission of green light is more phototoxic to cells, most scientists working on in vivo imaging models perfer...photoactivatable fluorescent proteins Some of the most important applications for PA-FPs involve enhancing...0.5-1% osmium tetroxide). These conditions destroy most PA-FPs, but, in 2015, the Looger Lab designed 2 ...

...range of parts, including copy number machinery, chromosomal integration modules, and many more of the usual...conditional gene expression, multiplexed CRISPR, and chromosomal integration of larger expression cassettes (Shaw...addition to additional elements for S. cerevisiae chromosomal integration and gene expression, the system includes...both targetability and effectiveness. Perhaps the most intuitive CRISPR MoClo application is to assemble...

...experiment. You may find yourself defaulting to the most popular or familiar options without even realizing...directly impact the way you can utilize these dyes. The most-used fluorescent dye is fluorescein isothiocyanate...matched with the experimental setup and objectives. Most systems can only excite and read a set number of...such as superior brightness, photostability, and most importantly, a photoswitching ability. This photoswitching...