We narrowed to 1,039 results for: LAS

...for an estimated 2% of global plastic generation. Less than 9% of plastics are ever recycled, so adopting...mail. Recycle plastic packaging Packaging is a huge contributor to the increase in plastic manufacturing...services don’t offer plastic recycling, specialized companies can recycle plastic-- you just have to find...other scientists save resources by sharing plasmids Find plasmids at Addgene ...recycling! With the popularity of single-use items, plastic is a substantial portion of lab waste. Despite ...manufacturing, accounting for 40% of total plastic usage (Geyer et al., 2017). While most single-stream or city ...Corning takes back and recycles a lot of their plastic packaging (from Corning®, Falcon®, or Axygen® product...

...multi-subunit complex employed by class 1 systems. A putative new class 2 nuclease, Cpf1 (CRISPR from Prevotella...endonuclease of a class 2 CRISPR-Cas system." Cell (2015). PubMed PMID: 26422227. Find plasmids from this publication...: Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system. Figure from Zetsche et al.,...., 2015. How was Cpf1 found and tested? Class 2 CRISPR systems, including the type II Cas9-based system...Francisella), was annotated in several genomes and is classified as a type V CRISPR system. Like Cas9, Cpf1 contains...Francisella Cpf1 (FnCpf1), they employed an E. coli plasmid depletion assay to discover FnCpf1’s PAM sequence....1 (2016): 31-34. PubMed PMID: 27918548. Find plasmids from this publication at Addgene. 3. Kim, Daesik...

...CRISPR mediated genome engineering. 10. Plasmids 101: Control Plasmids Need help thinking about the types ...Scientists eBook Download the Plasmids 101 3rd Ed. eBook Read the Most Recent Plasmids 101, CRISPR 101, and Career... two eBooks (Management for Scientists and the Plasmids 101 2nd ed, the 3rd edition has now been released...top of the many developments in science and the plasmids, technologies, and researchers behind them. As...most popular posts continue to be those from our Plasmids 101 and CRISPR 101 series. These posts aim to ... the background they need to get started using plasmids and the revolutionary CRISPR technology in their...and how it’s used in CRISPR genome editing.   2. Plasmids 101: Cre-lox Cre recombinase can be used to generate...

...background via P1 transduction. Plasmid Expressing lambda red genes from a plasmid allows for a mobile recombineering... flexibility to modify the E. coli chromosome, plasmid DNA or BAC DNA.  To use the lambda red recombineering...bacteria with integrated defective prophage 2) from a plasmid, 3) from mini-λ or 4) from the lambda red phage...phage pL promoter.  It is a good idea to choose plasmids that also express the repressors associated with...limit leaky expression of the Red system.  Using a plasmid to express the lambda recombineering system is ... do this is to express lambda red genes from a plasmid with a heat sensitive origin of replication.  Once... them at 42℃.  Mini-λ A hybrid between using a plasmid and stable integration of a defective prophage ...

...developed the plasmids, pPSU1 and pPSU2. When restriction digested with PstI or EcoRV, these plasmids generate...Penn State Ladder: Homemade Plasmid Prep Penn State Ladder: Commercial Plasmid Prep Kit Amount 1000 Lanes...Two plasmids that can be used to make inexpensive 100 bp or 1 kb DNA molecular weight ladders were recently...polyacrylamide gels. Get Tips on Verifying Your Plasmid Table 1: Comparing Typical Comericial Ladders...are significantly cheaper. When using a homemade plasmid prep protocol, it cost less than $10 to make 1000... 100 bp or the 1 kb ladder. Using a commercial plasmid prep kit increases the costs to $20-30 per ladder...prep protocol here and the pPSU1 and the pPSU2 plasmids are available from Addgene. Learn How to Analyze...

... labs and hinders experiments is mycoplasma contamination. Mycoplasma contamination has been shown to ...routinely test for mycoplasma contamination. There are several commercially available mycoplasma detection kits...larger microbes such as yeast, fungi, or bacteria, mycoplasma can be extremely hard to detect with levels reaching...cells per ml before the media becomes cloudy. Mycoplasma compete with host cells for nutrients and can...the cell line’s growth and behavior. Signs of a mycoplasma contamination can be subtle and may include reduced...positive controls. Routinely test your cell line for mycoplasma and discard it if the culture has become overly...after transfecting with the transfer and packaging plasmids. A benefit of the multiple harvest strategy is...

...loved helping to answer your plasmid and cloning questions through our Plasmids 101 series and by responding...Melina Fan Plasmids 101: The Promoter Region – Let's Go! By Kendall Morgan Plasmids 101: Mammalian...Joanne Kamens Plasmids 101: Antibiotic Resistance Genes By Marcy Patrick Plasmids 101: Yeast Vectors...FAQs Answered! By Caroline LaManna Plasmids 101: What is a plasmid? By Margo R. Monroe Best wishes...as seen through the lens of Addgene’s blog and plasmid repository. Our blog is just over a year old, and...detailed information about this new technology. Our Plasmids 101 series continues to grab scientists’ attention...Genome Editing By John Doench (Guest Blogger) Plasmids 101: Origin of Replication By Kendall Morgan ...

...Chromatin-related genes with plasmids available from Addgene The links below pull up lists of plasmids containing the...containing lists of plasmids from the indicated publications. You can request all of these plasmids directly from...consolidate every conceivable chromatin-related plasmid in a collection that grows on a daily basis, the... does not have it. That said, if you know of a plasmid that we definitely don’t have that you really want...study chromatin and want to start sharing your plasmids through Addgene, don’t hesitate to start the process...these pages to learn when Addgene receives new plasmids containing these genes. As with the PIs above,... In-exhaustive Chromatin-related genes with plasmids on Addgene.org AGO1 BRCA1 CREBBP CUL1 H2AFX...

...CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...Read the paper in Science Find the new SHERLOCK plasmids More precision with dual-nuclease Cas9-Cas9 ...Nature Communications Find the Cas9-Cas9 chimera plasmids A modular toolkit for genome editing in plants...post Read the paper in Science Find the Cas14 plasmids Anti-CRISPR proteins for optogenetic control...paper in Nature Methods Find the anti-CRISPR plasmids Tagging human transcription factors with GFP...White’s lab recently deposited a set of CRISPR plasmids to tag human transcription factors with GFP. As...

...our plasmid collection page Find more plasmids and resources for plant resources on Plant Plasmids Collection...box” for its common use in plasmid manipulation and genetics research. Plasmids are ideal tools for studying...Model Organisms blog post Read more about plasmids in our Plasmids 101 blog series Learn more about CRISPR...disease models.  Check out Addgene’s collection of plasmids containing Rattus norvegicus genes! Mexican axolotl...several vectors for use in the axolotl including plasmids for use in CRISPR and Tol2 transposase induced... and after embryogenesis. Addgene has over 600 plasmids that contain Xenopus genes and a tool-kit from...transformations and the use of Arabidopsis in the classroom on our blog. Now that you’ve learned more about...

... were in the cytoplasm of dendrites, and only a small proportion were found on the plasma membrane (top...to gold particles localized in the cytoplasm or bound to the plasma membrane. Note that the use of the... the DREADD receptors need to be located on the plasma membrane to be activated by the DREADD actuator...membrane in mice neurons, most of them were still cytoplasmic. Use of ‘small’ tags to identify proteins Given... the limited expression of hM4Di‐mCherry at the plasma membrane in monkey neurons, we next hypothesized...might improve the transport of this DREADD to the plasma membrane. The haemagglutinin (HA) tag is a small...tag resulted in abundant expression of hM4Di in plasma membranes in both species (bottom panel in Fig....

...service features 210 plasmids: empty backbones, fluorescent protein tags, SARS-CoV-2 plasmids, etc. It is our...to Elute and Store Your Plasmid DNA? by Paolo Colombi Should I store my plasmid in TE, Tris, or water?...Here we cover five methods to do this. 5. Plasmids 101: Plasmid Incompatibility by Leah Schiesow If you're... We've had 11 blog posts on COVID-19 featuring plasmid tools from depositors who quickly shared their ...you're thinking about hosting multiple plasmids in one cell, it might be that you can't stably maintain...This blog post features two mechanisms that cause plasmids to be incompatible. 4. RNA Extraction Without...CRISPR to detect SARS-CoV-2, including many with plasmids deposited at Addgene! 2. DNA Purification Without...

... target a cell class in mice is not a good predictor for targeting the same cell class in NHPs (R=0.38... target a cell class in monkeys was a good predictor for targeting the same cell class in humans (R=0.67... GFP marker (CatCH-GFP). The lab deposited AAV plasmids containing the active promoters at Addgene. Performance...specific cell types (ex: rods or cones) or cell classes (ex: photoreceptors which encompassed both rods...targeting More than 70% of expression was from a single class of cells  Of the four different promoter designs...of the retina.  Find the AAV synthetic promoter plasmids! Comparing synthetic promoters performance in ...the odds of that promoter targeting the same cell class in all three species are still greater than random...

... in E. coli with two plasmids (Figure 3 below): Input Plasmid: The input plasmid (high copy, ColE1 origin...inducible promoter (PPhlF).  Output Plasmid: The output plasmid (single copy, bacterial artificial chromosome...through Addgene. Their plasmid maps and GenBank files can be found on their plasmid pages. All two-input...indicated figures in our paper (1)) Input Plasmid Output Plasmid Fig. 3A pNR64 pNR160 Fig. 4A pNR220...Materials and Methods section of our paper (1). Lastly, it is possible to build gene regulatory RSMs (...two-input RSMs and three three-input RSMs. The plasmids used to construct these RSMs are listed in the...paper (1). These parts are also annotated on the plasmid maps and GenBank files uploaded to Addgene. We ...

... tube is mislabeled or someone grabs the wrong plasmid? What if bacteria snuck into the prep during the... human error, fragments of DNA from the helper plasmids or the cell genome can be packaged inside the ...) are aligned to the reference sequence of the plasmid used to create the viral prep. In a clean viral...this reference sequence. Second, we perform a megaBLAST search on all the reads that did not map to the...bacterial genomes, cloning vectors, and helper plasmids (Chadeuf et al., 2005, Wright et al., 2011). In...correspond to the contaminant. Each contig is then BLASTed against the NCBI nucleotide collection database...database, and it may also be aligned to plasmids in our inventory containing the suspected contaminating sequence...

...in a 1-plasmid system, in which Cas9 is included on the gRNA-containing plasmid, or a 2-plasmid system...contain thousands of gRNA plasmids, discerned only by a unique barcode on each plasmid. As such, sequencing...are composed of many different gRNA-containing plasmids, each with a different genomic target. The goal...sequences and ease of generating gRNA-containing plasmids. CRISPR genome wide-screening experiments commonly... target every gene in the genome, or a specific class of genes? Addgene currently carries several genome-wide... selection of sub-libraries targeting specific classes of human genes. What are the steps involved in ...other types of experiments, check out our CRISPR Plasmids and Resources Page. References McDade JR, Waxmonsky...

...Baird, who has created plasmids as a technician in the Davidson lab for the last four years. “Researchers...Molecular Expressions collection including some 3,300 plasmids along with image galleries and educational resources...resources to go with them. This collection of plasmids is available in an easily searchable format on Addgene...Fluorescent Protein Collection webpage. These plasmids are divided into the following categories: Over...list of cell markers all tagged with mEmerald. Plasmids to monitor protein-protein interactions through...Specialty constructs for biosensing. Over 300 plasmid backbones spanning a rainbow of fluorescent proteins...Proteins - Making the Right Connection.  mEmerald plasmids and many more! Baird has a helpful tip for anyone...

...bacteria with the plasmid can grow. As the bacteria divide, they replicate and retain the plasmid. This allows... Inside Addgene Posts! Learn all about Plasmids in our Plasmids 101 series! ...Every plasmid sample enters Addgene the same way. A package is delivered by a mail courier and then the...about Developing Lab Management Software    The plasmid's journey begins First, each package is opened and... to hundreds of samples, with an average of 10 plasmids per deposit. Each week an average of 16 deposits...allow us to store multiple types of tube for each plasmid we have at Addgene. Each sample at Addgene has ...strains that have been treated to easily take in plasmids (transformation). Transformed bacteria are then...

...For the last 14 years, scientists have been coming to the Addgene website in search of plasmids. Now, scientists...synthetic biology page Search for a plasmid Find Addgene's most popular plasmids ...Addgene as a large data set. Addgene has over 65,000 plasmids in the repository, each verified by sequencing...at its sequence. MIT collaboration for gene classification This inquiry started when Neil Thompson, a ...is synthetic or natural with 97.7% accuracy. Classifying genes as natural or synthetic But how can you...organism, it may express protein more efficiently. To classify gene sequences as natural or synthetic, the researchers...simulations and machine learning, they created a classifier that predicts whether a gene was natural or synthetic...

...Would you buy and use a plasmid if it didn’t include any plasmid sequence data? Of course not. Unfortunately...depositing your plasmids encoding antibodies, ScFvs, and nanobodies to Addgene! The plasmids will be made...Plückthun, 2015a, b). Recombinant antibodies are plasmid-based and offer numerous advantages, including ...antibodies that you have come to expect from our plasmid and viral services. The first step will be working...working with the community to build a resource of plasmids encoding recombinant antibodies as well as other...through our usual service (see our current antibody plasmid collection) and we plan to introduce affinity reagents...through distributions of research materials, such as plasmids and viral vectors. However, it typically takes...