...generated using random genomic fragments from the organism of interest, or a defined cDNA library (e.g. the...number of two-hybrid systems available in model organisms, including bacteria, alternative fungi (C. albicans...Enzymol. 1983;100:293-308. PubMed PMID: 6312261. 2. Keegan, L., Gill, G., and Ptashne, M. Separation of DNA...

... that the drug prevents the growth of a living organism. As for multi-drug resistance: definitions vary...vary in the literature, but generally organisms with multi-drug resistance are resistant to three or more...is an intense amount of competition between microorganisms for available resources. This competition drives...

...over-express coding sequences from a given cell type or organism, have been used in a similar manner to Cas9 activators...lines (HEK293T, MCF7, U2-OS, Hela, N2A, 3T3) and organisms (Chavez et al., 2016). To learn more about the...Tuttle M, P R Iyer E, Lin S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, Davidsohn N, Housden...

...one either. Choose the topic and outline the organization of the review Once you start reading, there ...references) and it’s better to keep your references organized from the beginning. I also recommend using the...Manuscripts: Literature Searching, Reading, & Organizing Tips for Improving Your Next Manuscript 6 Tips...

... number of career paths--industry, nonprofit organization, law office, journal editor, etc.--so everyone...around the world, such as BosLab here in Boston. Organizations like this one are always looking for scientists... own independent research!). There are also organizations like Science from Scientists, which try to bring...

...(CNS) orchestrates complex processes enabling organisms to control their movements and behavior. These...to study connectomics (how neural circuits are organized and how neurons connect to each other). Combined.... 2. Shepherd, G.M. (2004). In "The synaptic organization of the brain". Oxford University Press, New ...

...distinct subcellular environments, including the organelles that compose the secretory pathway, endocytic...formation, and proteolytic cleavage. FPs localized to organelles are equally susceptible to these non-native modifications... Lee Snapp. "Fluorescent proteins in cellular organelles: serious pitfalls and some solutions." DNA and...

...non-human primates [7, 8], zebrafish [9], and c. elegans [10]. If used properly, optogenetics is sure to...Cellular and Molecular Biology from the University of Michigan and he is currently a Postdoctoral Fellow in the...neural activity in freely moving Caenorhabditis elegans." Nature methods 8.2 (2011): 147-152. PubMed PMID...

...your host organism. Because transcription machinery differs between cell types or organisms, promoters...  Kendall Morgan...

... to living cells thus APEX is not suitable for organoid or in vivo studies (Che and Khavari, 2017). Find...many fusion proteins this would lead to protein/organelle aggregation. The Ting lab hypothesized that the...Efficient proximity labeling in living cells and organisms with TurboID." Nature biotechnology 36.9 (2018...

...the observation of proteins in living cells and organisms. Both components of the chimera are encoded by...). However, these assays are qualitative. The Organized Smooth Endoplasmic Reticulum (OSER) assay (Costantini...hindrance by the fluorescent protein. Thus, scientist began using relatively long linkers between the protein...

... were identified using systematic evolution of ligands by exponential enrichment (SELEX). After 4-6 rounds...single molecules and for live-cell imaging in C. elegans. Other groups have used Mango to study RNA-protein...PubMed Central PMCID: PMC3852148. Warner, Katherine Deigan, et al. "Structural basis for activity of highly...

...has also been implemented in a variety of other organisms, such as sphingomonads and bacillus.  ADH1 The...shown that the Q system is also functional in C. elegans. Subsequent work by the Luo and Potter labs has...repressible binary expression system in Caenorhabditis elegans.” Nat Methods 9(4) (2012): 391-5. PubMed PMID: ...

...signaling molecules, transcription factors, and ligands. The presenter then showed which genes they had...that were essential for the activity of different ligands. Such knowledge could be very useful for drug discovery... to block the cellular response to a particular ligand (such as when a component of a microbe or a cytokine...

... blood stream to commute to and invade multiple organs, and most importantly escape the immune mechanisms...Cancer cells also produce programmed cell death ligand (PD-L1) that inhibits the cytotoxic activity of...environments for tumor cells spreading to other organs during metastasis. Evading Recognition Tumor cells...

... MC, Metzger A, Titus KR, Gilgenast TG, Gong W, Beagan JA, Raj A, Phillips-Cremins JE (2019) LADL: light-activated...Biology. Springer US, pp 261–281 Manna D, Maji B, Gangopadhyay SA, Cox KJ, Zhou Q, Law BK, Mazitschek R, Choudhary...for remote-controlled genome editing of internal organs and tumors. Sci Adv 6:eabb1777 . https://doi.org...

...from Science in the News, a graduate student organization that works to promote science communication....non-scientists. These programs are all run and organized by graduate student researchers. That’s right,...

...Plant Breeding, Genetics, and Biotechnology at Michigan State University with the backing of a prestigious...:47-9:35 : Kingdom's biotechnology research at Michigan State University 9:36-10:29 : Kingdom's transition...

... data on the date, material, requesting PI & organization, and country. This information can be of personal...data only, information on requesting PI(s) & organization(s) is not shown on the dashboard. Key features...

...Plasmids 101: Origin of Replication By Kendall Morgan Save Time and Money by Making Your Own Competent...101: The Promoter Region – Let's Go! By Kendall Morgan Plasmids 101: Mammalian Vectors By Marcy Patrick...