... Joining the Addgene crew!  This pattern was consistent: everyone I spoke with at Addgene, no matter their...hunting and interviews, I accepted a position at Addgene as a marketing and social media co-op. There are...realized I had made the right decision to choose Addgene for my co-op, but I know it didn’t take long. It... It may have been at one of my first “All Addgene” meetings, when Melina, our CSO and co-founder, approached...analytics, something that had never been done at Addgene before. This meant I was responsible for establishing...understand what metrics would be useful and how to organize the metrics in a digestible manner.  At first,...strategy. I really believe that the culture at Addgene cultivates trust between coworkers and that trust...

... 5’ of the gene they control. Promoters can be organism or tissue type-specific, which can help you restrict...10.1089/hgtb.2019.276 Additional resources on the Addgene blog Learn more about basic viral vector elements...cell-type expression in retinal cells Resources on Addgene.org Find AAVs for your experiment Browse our AAV...

... that mimic aspects of organ morphology and physiology in vitro and have emerged as a powerful model for...find all of the CRISPR plasmids available from Addgene, head over to our CRISPR Plasmids and Resources... CRISPR-based genome editing in organoids By Ashley Waldron Organoids are three-dimensional cultures that...toolkit for performing genetics studies in organoids. The Organoid EasyTag System is a straightforward workflow...in an organoid model (Sun, et al. 2021).  Figure 1: Example schematic from the Organoid EasyTag...fetal-lung derived organoid system, the approach is intended to be amenable to organoids derived from other...find in this article: CRISPR-based editing in organoid Biosensors for detecting strigolactones The CRISPR...

...transposons can be used to both introduce genes into an organism’s genome (see figure) and to disrupt endogenous...10.1111/imr.12137. PubMed. Find the Plasmids at Addgene Kowarz Lab plasmids Izsvak Lab plasmids  ...

...understand the part they play in the goals of the organization and feel the importance of their contributions...Managing People for Scientists. Subscribe to the Addgene Blog to follow the entire series as it is published...valued This often stems from being included in organizational decision-making. It is imperative that employees...

...where they are. 8)   Look into training from an organization such as COMPASS. Although COMPASS has a special...programs at both MIT and Boston University. At Addgene we're committed to scientific sharing – both your... Kendall Morgan...

...capability of transforming the genetic code of living organisms and its societal implications even before the ...pneumoniae bacteria could be transformed from one organism to the other. This is now famed as the Griffith...at @kartik_lakshmi. Additional resources on the Addgene blog Find more blog posts about CRISPR Learn more...about other genome editing methods Resources on Addgene.org Visit our CRISPR guide Find CRISPR plasmids ...

...out full plasmid sequencing at Addgene Additional Resources on Addgene.org Example plasmid analysis powered...annotation gaps in the plasmids available through the Addgene website. In addition, two initiatives are in the...is to enhance Collections so that a group or organization can annotate their sequences from a shared database...University of Chicago. He was an early depositor at Addgene, and conceived SnapGene because he needed it for...post-launch iterations.     Additional Resources on the Addgene Blog Read about our SnapGene powered plasmid maps...feature database. Powering the plasmid maps on Addgene.org with SnapGene Server is useful because it helps...

...Twitch calcium sensors, which are available at Addgene, can be used for ratiometric in vivo imaging. Griesbeck...and zebrafish – essentially all of the standard organisms for which extensive genetic tools are available...the first of its kind. “I hope by depositing at Addgene, people will start using them,” Merkx said. “For...(PubMed) Interested in More Hot Plasmids from Addgene? Check out our Hot Plasmid page, where we highlight...some of the popular & newly available plasmids at Addgene. If you're interested in plasmids for in vivo imaging... Kendall Morgan...

...received matches the expected sequence. Here at Addgene, we use NGS-based quality control to confirm the...the plasmid based on the predicted sizes and organization of different features within the plasmid. Restriction...necessary but also an easy process. Please vist Addgene's resource for plasmid verification to find additional...DNA gel.  More Plasmid Eductional Resources: Addgene's Molecular Biology Reference Find More Protocols...

...multi-color imaging. Find the HA frankenbody tool-kit at Addgene to get started with your experiments! What else... frankenbody can be used for imaging in whole organisms. The researchers used the HA frankenbody to successfully...PMCID: PMC6610143. Additional resources on the Addgene blog Learn about visualizing protein turnover ...fluorescent protein blog posts Resources on Addgene.org Read our fluorescent protein guide Find plasmids...

...that selection of a fluorescent probe (be it an organic dye or FP) is one of the most important steps in...excellent series of posts by Joachim Goedhart on the Addgene blog – outlining various important considerations...github @tlambert03.   Additional Resources on the Addgene Blog Fluorescence Microscopy Techniques - Which...Proteins for Multicolor Imaging Resources on Addgene.org Browse all fluorescent protein plasmids Find...

...Career Blog Posts Resources at Addgene.org Find Job Openings at Addgene ...fascinating biology is found in weird and rare organisms so I found it hard to stick to vertebrates alone...Pam_Hines and LinkedIn. Additional Resources on the Addgene Blog What Do I Do Now? Academic v. Non-Academic...

...plasmids from this publication at Addgene! Additional Resources on the Addgene Blog Find planarian tools for...without the need to handle a living, replicating organism. Furthermore, cell free systems can be freeze-...center brings plant science to life Resources on Addgene.org Browse our protocol videos to learn simple lab...

...All organisms share an innate goal to survive. This past year, scientists hijacked survival tactics of...innovative biological and translative technologies. Addgene has empowered researchers to harness previous experimental...For more information on CRISPR/Cas, check out Addgene’s CRISPR Guide or find CRISPR/Cas plasmids for your...

...expand Addgene’s services. There was an overwhelmingly positive response to the option of Addgene providing...allows us to maintain Addgene’s high quality standards?” Under the leadership of Addgene’s co-founder, Melina...In the middle of 2016 Addgene started distributing a small but growing catalog of ready-made AAV and ...Lentiviral preps. This new Viral Service represents Addgene’s largest new initiative since we started distributing... would like to thank some of the people and organizations who helped us reach this milestone. Viral service... capabilities to produce and distribute virus. Addgene is a 501(c)3 nonprofit and our plasmid service ...build a completely self-supporting service so that Addgene is not dependent on grants. Aiming to operate under...

...help guide and promote Addgene in its mission to make research easier. Addgene is excited to announce ... is what got him involved with Addgene - he says he’s “known of Addgene basically since the beginning ...ethic that Addgene has behind it is something that very much resonates with me.” About Addgene’s role in...Doench says, Addgene is “a central resource for what is worth having. The work that [Addgene’s] outreach ...do and to organize it and catalogue it, I mean that’s the critical function really…. Addgene’s role as ...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.       Resources... The Addgene Advisory Board consists of prominent members of the bioscience and tech transfer communities...

...on the Addgene Blog Learn More About the Addgene-Seeding Labs Plasmid Grant Check Out Addgene-Seeding... scientists globally, Addgene has partnered with various community organizations and workforce projects...of the Addgene-Seeding Labs Plasmid Grant awardees' projects progress. Subscribe to the Addgene blog for...Susanna Bachle and Ina Ersing Seeding Labs and Addgene collaborate to provide scientific resources, equipment...resource-limited settings. More specifically, the Addgene-Seeding Labs plasmid grant provide plasmids to ...to these researchers. In the third round of the Addgene-Seeding Labs plasmid grant we are excited to introduce...its position in the river back to the user.   At Addgene we're happy to be able to support diverse groups...

... to our viral vector pages on the Addgene website and on the Addgene blog to get some ideas for future...Neuroengineering Primer on Optogenetics Resources at Addgene.org Addgene's Viral Service Find Retrograde AAV Browse... Addgene recently attended the Society for Neuroscience 2017 conference in Washington DC (#SfN17). This...addition, there was an entire story-telling session organized by Story Collider and which featured a talk about...you can apply it in neuroscience. AAV tools from Addgene are already having an impact on the field Not to...horn too much, but many people who came to the Addgene booth (there were over 400 who signed up for our...virus (AAV) and lentivirus are now available from Addgene. While super useful, these vectors require a bit...

...’t. Try out the new Addgene search engine! Additional resources from the Addgene blog Learn about other...other features on Addgene's website Find a step-by-step guide to using Addgene's new search Read to learn... the different website navigation paths, the organization and completeness of our information, and the...posts or reagent items.  The technology behind Addgene’s new search  Like millions of other websites, we...data in particular and our deep understanding of Addgene’s customer’s needs. Categories We created a categorization...effort and cross-team collaboration (thanks to all Addgenies involved). The new homepage and navigational elements...