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Showing: 241 - 259 of 259 results

  1. Molecular Biology Protocol - Restriction Digest of Plasmid DNA

    Type
    Protocol
    ...ideal conditions with very clean DNA, so using a little more enzyme is advisable. Reactions are often performed...enzyme than you will need, but that's okay because a little more enzyme is usually better. Mix gently by pipetting...

  2. Pipetting Protocol

    Type
    Protocol
    ...Containers to hold measured liquid (ex: microfuge tube, bottle, etc.) Labels for containers Reagents Liquid for...measured liquid (ex: another microcentrifuge tube, a bottle etc.). If there is already other liquid in the ...

  3. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...PEI-MAX powder to 900 mL deionized water in a 1 L bottle and stir on a magnetic stir plate. Stir until all...into a clean microcentrifuge tube. Pro-Tip Cells settle quickly and need to be resuspended before sampling...

  4. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...X PBS, 1X pH 7.4, VWR 45000-446 250 mL sterile bottles, Corning 430281 Deionized water IgG isotype standard...LC) proteins, respectively. There should be very little background staining. Pro-Tip A large shift in the...

  6. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ... Pipettes, 10 mL, VWR 89130-898 1 L polystyrene bottle, Corning 430518 PBS, 1X pH 7.4, VWR 45000-446 TMB... mL of Tween-20 to into 999.5 mL 1X PBS Cap the bottle and invert several times to mix. Carefully remove...

  7. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...stable alternative, such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add 55 mL of heat-inactivated...especially in the corner of the well as cells tend to settle there. Once the cells have expanded but before ...

  8. DNA Quantification

    Type
    Protocol
    ...accurately read DNA concentration and purity in as little as 1μl. Regardless of whether you have a NanoDrop...

  11. Fluorescence Titering Assay

    Type
    Protocol
    ...stable alternative, such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add 55 mL of heat-inactivated...

  12. General Transfection

    Type
    Protocol
    ...stable alternative such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add 55 mL of heat-inactivated...

  13. Lentivirus Production

    Type
    Protocol
    ...stable alternative, such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add 55 mL of heat-inactivated...

  14. Colony Formation Titering Assay

    Type
    Protocol
    ...stable alternative, such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add 55 mL of heat inactivated...

  19. CRISPR Library Amplification

    Type
    Protocol
    ... of altering the composition of the library. Bottlenecks, fitness differences, and plasmid recombination...
Showing: 241 - 259 of 259 results