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  1. Summer SciComm Series: A PhD in Science Communication

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    Blog Post
    ...fitting that I’ve ended up (again) dispelling the same myths and misconceptions fifteen years later. What..., peer-reviewed study. The second group read the same abstract but with the names reversed, meaning that...
  2. 22 Hot Plasmid Technologies from 2014

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    Blog Post
    ...use of the two systems in different tissues of the same animal. Flipbow additionally incorporates SUMO tags...which express self-excising Cre recombinase from the same transgene as the Brainbow cassette, simplifying ...expression plasmids for a given species are based on the same backbone to permit expression levels to be directly...
  3. Clear and Accessible Writing

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    Blog Post
    ...that helps visually impaired readers receive the same information as sighted readers.    Figure...Excellent! We asked our workshop participants to do the same thing, and even though only about 10% of them had...
  4. Plasmids 101: Screens vs. Selections

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    Blog Post
    ...defined disease phenotype. These screens employ the same downstream manipulations as a forward or reverse...unlike in screens. Selections begin and end with the same steps as screens – they start with manipulations...
  5. Addgene’s Viral Service - Why Virus? Why Now?

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    Blog Post
    ...2004. We’ve already distributed over 500 viral samples to scientists all over the world. Now that the ..., tracked production and distribution of viral samples. The Office Team made sure we could ship internationally...
  6. Sweating the Small Stuff: Details in the Lab

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    Blog Post
    ...running it unchanged over and over again, and the same goes for your workflow. Next time you make a mistake... to match your plate layout can help you track samples as you add them. Moving your reagents from your...
  7. Antibodies 101: Buffers, Storage, and Conjugates

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    Blog Post
    ... the antibodies time-sensitive, using precious samples, or being compared to other data in a manner that...signaling molecule, that all need to be detected in the same assay. This is one of the most complex subjects ...
  8. Kiran Musunuru on the Newest TALEN Genome-Editing System

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    Blog Post
    ...of individuals and find several mutations in the same gene or in multiple genes, all of which seem to ...insert all of those variants into stem cells with the same genetic background and do an apples-to-apples comparison...
  9. Lighting Up Cell Signaling with Photoswitchable Kinases

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    Blog Post
    ...hypodermis and gut cells of C. elegans causes the same phenotype. Activation of the Raf-MEK-ERK signaling...signaling pathway with psRaf1 or psMEK1 in these same cells by 24-48 hours exposure to blue light also induced...
  10. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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    Blog Post
    ... the ccdB aspect, Gateway takes advantage of the same principle that cells will not propagate while expressing.... While these two strains ultimately perform the same function, there is some evidence that ccdB Survival...
  11. Viral Vectors 101: An Introduction to AAV

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    Blog Post
    ...episomal and does not divide and replicate in the same way the host DNA does. Because of this, fast dividing...McCarty, D. M., Paul E. Monahan, and Richard J. Samulski. "Self-complementary recombinant adeno-associated...
  12. The time and cost required to make a plasmid

    Type
    Blog Post
    ...overall inflation of USD has gone up 19.8% in the same time period. For ease of calculations*, let’s split...inflation at… roughly 34.9%, which means that the same plasmid would cost $155.25 in reagents and consumables...
  13. All in a Twist: dsRNA

    Type
    Blog Post
    ...systems can lead to local dsRNA production. In the same vein, genotoxic stress, such as chemotherapy treatment...dsRNA finds its way outside of the mitochondria, the same inflammatory response can activate. These types ...
Showing: 241 - 260 of 581 results