...about 100 bps into the run, it got “dirty”, with multiple sequence traces overlapping each other! When the... Wu X, Jiang W, Marraffini LA, Zhang F (2013) Multiplex Genome Engineering Using CRISPR/Cas Systems. Science...-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering...

...counts making them fast and viable options for multiplex sequencing in many laboratories. Nevertheless,...DSBs, doesn't require high read counts, fast multiplexing Requires delivery of dsODN (potentially toxic...dsODN), doesn't require high read counts (fast multiplexing) Requires large cell population, senstive to...

... protein because the antibodies will recognize multiple epitopes on the protein. However, each animal ...advantage of flow cytometry is the ability to use multiple fluorescent signals (up to a dozen or more!) to...relative or absolute amount of the expression of multiple proteins on the same cell simultaneously as well...

...Chloride Close the bottle and mix by inverting multiple times Adjust pH to ~8.5 Filter sterilize through...DMEM Complete. Pipet back and forth vigorously multiple times to obtain a single cell suspension (no clumps...

...KarplusIBEX Knowledgebase: A Data Resource for Multiplex Tissue ImagingResearchers: Andrea Radtke, Ziv ...Cynthia Arokiaraj; Viviana Gradinaru; Tim ShayMultifunctional Neuronal Fibers: Modulation from Gut to BrainResearchers...

...way to increase CRISPR editing efficiency across multiple CRISPR enzymes. Plasmids for CRISPR coselection...segment on regulating telomere length with TZAP Multiplexible plant genome engineering toolkit The Voytas ... well as Cys4, tRNA, and ribozyme systems for multiplex gRNA expression. Expression of toolkit components...

... they have developed this further to provide multiplexed detection, higher sensitivity, and more. Read...lab compared the efficiency and specificity of multiple wild-type and engineered variants of Cas nucleases...

... which display sophisticated interactions with multiple other proteins. Again, little to no change in ... place to start. These tags are easy to use in multiple common applications. Due to their limited effects...

...less experienced grads Manage a project involving multiple scientists, create and stick to timelines Be a...another field maybe, and publish Join a lab that has multiple collaborations, even with Industry labs Work on...

...feedback and input on multiple topics every time you meet.  Seek feedback in multiple formats      Ask ...

...e.g. SNPs) exist. Additionally, consider testing multiple gRNAs targeted to the same locus to ensure their...elegans gRNA sequences. You can also filter by multiple keywords that are separated by a single space ...

...protein purification through simple and efficient multiplexing. pCri plasmids are divided into two sets, each...schematic below), enabling a researcher to test multiple tags for one protein quickly and easily. These...

...both colors allows for increased options for multiplexing in experiments, including ones that study alpha2...Reiner, A., … Patriarchi, T. (2023). Sensitive multicolor indicators for monitoring norepinephrine in vivo...

....1918.) Near-infrared fluorescent proteins for multicolor in vivo imaging. Shcherbakova and Verkhusha (...10;4:2153. doi: 10.1038/ncomms3153. PubMed) Multicontrast photoacoustic in vivo imaging using near-infrared...

...as Slack. Share data and documents While having multiple scientists edit the same document may sound like...collaborators. Being able to present data from multiple aspects of a collaboration and immediately discuss...

...for selection of plasmid-containing bacteria. Multiple Cloning Site (MCS) Short segment of DNA which ...maintain your plasmid stocks. The guide also contains multiple protocols and troubleshooting tips to make plasmid...

...Development-specific genes – if a gene functions at multiple stages in development, its depletion may result...be observed. Functionally redundant genes – multiple genes which function in overlapping roles can ...

...frequently used in molecular biology to isolate, multiply, or express the insert they carry in the target...has the CMV promoter for mammalian expression, a multiple cloning site to insert YGOI, a bacterial selection...

...biosynthetic pathways. Biosynthetic pathways containing multiple enzymes could be successfully reconstituted in.... The lab demonstrated that XMAS-TREE can be multiplexed and can be used in human pluripotent stem cells...

...modular, selection-based gene tagging approach Multiple features of C. elegans have made CRISPR/Cas9 so...HDR mentioned above, an accessible germline, and multiple selectable markers. Bob Goldstein’s lab previously...