Hot Plasmids and Viral Preps - January 2021
Type
Blog Post
...bringing the C-terminal and N-terminal Cre fragments together and permitting recombinase activity. Opto-Cre-Vvd... of bacterial gene expression
By Meghan Rego
Recombinases are enzymes that recognize, cut, and alter specific...translocation or cassette exchange. By utilizing recombinases to manipulate genomes, scientists can control...expression of their genes of interest. While recombinases are powerful gene expression tools in and of...Cells expressing an RFP reporter plasmid for recombinase activity were exposed to blue light. Cre-Vvd ...substantially improved activity as compared to other recombinase/photodimer pairs. Created with BioRender.com
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Hypercompact genome editor CRISPR-Casɸ
By Melina Fan
The Doudna Lab adds to the CRISPR-Cas toolbox...