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  1. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this....org for more information and assistance with the deposit process. Want more information on the wide variety...publication or plasmid information page, or contact the depositing PI for more information on the experimental...publication associated with each plasmid for more information on how it was originally used. Here are a few...
  2. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...let us know using the Cloning Grade DNA Suggestion Form . Please note that this does not guarantee that ... our collection. The plasmids available in this format include a variety of popular plasmids and backbones...individual plasmid page to find current pricing information Standard Quality Control Plasmid verified by ...Industry PI FAQ What Quality Control analyses are performed on my cloning grade DNA order? For each cloning...DNA preps of this plasmid available, but helps to inform us of which plasmids would be most helpful to requesting...
  3. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...ligase complex. Finally, when auxin is added in the form of auxin hormone indole-3-acetic acid (IAA), the...behave with a new protein, but you can make an informed choice! References and Resources References Bondeson...
  4. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...exogenously to measure a phenotypic response in the form of fluorescence or voltage indicators.   Viral... here!). The only issue with these GEVIs? They perform poorly in two-photon conditions, which are necessary...
  5. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...transsynaptically). This is because the recombinant forms of these viruses typically used in research aren...after, we learned about organs and the jobs they perform. This became foundational later on when we studied...
  6. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    ...case, the larva will metamorphose into its adult form. In all, it takes roughly 10 days for an egg to ...Some resources will direct you to the latest information about gene function, while others offer fly stocks...Flybase.org is absolutely essential. You can look up information about your fly gene using the search box in the... started. Any given gene’s webpage will have information on where the gene is expressed within the fly...website as well. Additionally, they have useful information on topics such as choosing the right balancer...
  7. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...MMEJ (microhomology-mediated end-joining)? MMEJ, a form of alternative end-joining, requires only very small...’s become clear that the power of this editing platform lies in the diversity of nucleases and their applications...
  8. Neurodegeneration Research Collection

    Type
    Collection
    ...or after a person's mid-sixties. The early-onset form accounts for less than 10 percent of all cases of... of Alzheimer's disease. Of these early-onset forms, a fraction of these are due to a mutation in one ...NeuroMabSeq platform. Mitchell et al. Sci Rep. 2023 Sep 27. See More Fluorescent Proteins Find information on ...has assembled the following to help you find information and plasmid collections that may help with your...Differentiation Factors Find plasmids used to transform iPSCs and fibroblasts into neurons and more. New...Commun. 2023 Dec 5. See More Antibodies Find information on Addgene's collection of plasmid-based antibodies...Link opens in a new window) A news website and information resource dedicated to helping researchers accelerate...
  9. AAV Molecular Tools

    Type
    Collection
    ...fill out our form to let us know what you would like to see available in viral prep format. Please note...
  10. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type
    Blog Post
    ...protein interaction partners (PIPs), the FP fragments form the complete FP because the PIPs they’re tethered...to bring them into close proximity (Figure 1). Formation of the complete FP results in a fluorescent signal...selection of FP fragments and, preferably, some information about the structures of the tested PIPs. Many...fuse the FP fragments. In the absence of this information, you can fuse each FP fragment to the N- and ...your results. Unfortunately, without structural information, constructing appropriate controls may requires...
  11. Hot Plasmids - August 2020

    Type
    Blog Post
    ...synthetic oscillator, bistable network, stripe pattern-forming incoherent feed-forward loop. Find the plasmids...systems at Addgene! Schematic of the evolution platform to generate TEV protease variants. Catalysis by...the right places, which can provide misleading information on where exactly a structure or protein is located...dissolved at a higher concentration, and results in uniform NB biotinylation. Once induced, the NBs could be...developed fluorescently labeled piggyBac vectors for uniform and sustained expression of single or multiplexed...
Showing: 241 - 260 of 963 results