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  1. Worm Expression Resources

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    Collection
    ... individually. CRISPR Read Addgene's CRISPR Guide for a general introduction to CRISPR technology. Addgene's... tag insertion using CRISPR/Cas9 and the selection of C. elegans strains. CRISPR plasmids from John Calarco's...genome editing in C. elegans via a CRISPR-Cas9 system. Cloning-free CRISPR for C. elegans , which uses purified...Browse plasmid tools, kits, and resources (such as CRISPR and synthetic biology collections) for use in C... feeding worms bacteria expressing dsRNA or use CRISPR/Cas9 gene editing to easily engineer specific transgenic...contain tools for use in worm research. This includes CRISPR, RNAi, empty backbones, fluorescent proteins, and...Addgene's worm CRISPR resources include plasmids expressing Cas9, empty gRNA backbones, and constructs ...

  2. CRISPR Plasmids - Parasites

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Eukaryotic... CRISPR Parasites CRISPR Plasmids... Libraries Toxoplasma CRISPR Knockout Pooled Library - Toxoplasma gondii CRISPR genome-wide knockout gRNA...CRISPR expression plasmids that have been designed for use in parasites. CRISPR...Plasmids: Parasites Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional... research for decades. Recent developments with CRISPR-Cas9 gene editing have propelled both reverse and... forward genetics of these parasites. Moreover, CRISPR-dCas9 gene regulation has also been established...

  3. Plasmids 101: Golden Gate Cloning

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    Blog Post
    ...efficiently in just a few steps. More recently, CRISPR technology has adapted Golden Gate cloning for ... you are using the Golden Gate method to create CRISPR/Cas9 constructs, assemble standard plasmids parts... Check out Addgene’s restriction cloning guide! CRISPR 101: Multiplex Expression of gRNAs Choosing a Molecular...

  4. CRISPR Plasmids - Purify Genomic Loci

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Identifying... CRISPR Purify CRISPR: Purify Genomic ...Catalytically inactive CRISPR plasmids for purification of target loci. CRISPR...Genomic Loci Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...essential to understanding locus function. Using CRISPR, researchers have expanded chromatin immunoprecipitation... conventional methods for genomic purification, CRISPR-based purification methods are more straightforward... with a genomic region of interest in vivo. For CRISPR-based purification, design your gRNA sequence to...

  5. CRISPR Plasmids - Epigenetics

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    ... CRISPR Epigenetics CRISPR Plasmids: Epigenetics Browse CRISPR Plasmids By Function...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts To...CRISPR plasmids for targeted epigenetic modification, including histone acetylation/demethylation, and...and are potentially heritable in dividing cells. CRISPR Activate and Interfere technologies also indirectly...enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity... . Browse, sort, or search the tables below for CRISPR plasmids for epigenetic modification in mammalian...

  6. CRISPR Plasmids - Tagging

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... CRISPR Protein Tagging CRISPR Plasmids...Read our CRISPR 101 post for more information about HDR/repair template design. How to use CRISPR to tag...for a CRISPR-based system to add a tag (currently using FLAG) to endogenous proteins. This CRISPR/Cas plasmid-system...SapTrap CRISPR/Cas Toolkit SapTrap is a modular toolkit from Erik Jorgensen's lab for building CRISPR targeting...CRISPR plasmids for endogenous tagging of your gene of interest. CRISPR...Plasmids - Protein Tagging Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit...

  7. CRISPR Plasmids - RNA Editing

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type... CRISPR RNA Editing CRISPR Plasmids...CRISPR plasmids based on Cas13 designed to precisely edit RNA targets. CRISPR...Plasmids: RNA Editing Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional... Type VI CRISPR systems, including the enzymes Cas13a/C2c2 and Cas13b, target RNA rather than DNA. Fusing... of Cas enzymes? CasPEDIA is an encyclopedia of CRISPR systems with wiki entries describing enzyme activity... . Browse, sort, or search the tables below for CRISPR plasmids for RNA editing in mammalian systems. ...

  8. Calarco Lab CRISPR Plasmids

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    ...Lab CRISPR Plasmids Available from Addgene You may also like... CRISPR Guide CRISPR Protocols gRNA Design... CRISPR Calarco Lab CRISPR Plasmids Calarco Lab...Design Tools CRISPR Blog Posts A CRISPR/Cas system designed and optimized for work in C. elegans . The...CRISPR Cas9 gRNA plasmids from Dr. John Calarco's lab. CRISPR...Heritable genome editing in C. elegans via a CRISPR-Cas9 system. Friedland AE, Tzur YB, Esvelt KM, ...

  9. Microbes: Look, Listen, and Tell

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    Blog Post
    ...Resources on the Addgene Blog Read Our Post on CRISPR Methods for Bacterial Genome Engineering Learn ...Addgene.org Check out Our Microbiology Resource Page CRISPR plasmids designed for use in bacteria Deposit Your...

  10. CRISPR Plasmids - gRNAs

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    ... CRISPR gRNAs CRISPR Plasmids: gRNAs Browse CRISPR Plasmids By Function Genome...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...PAM sequence. Which CRISPR application is this gRNA sequence compatible with? CRISPR knockout experiments...experiments use targeting sequences within exons, whereas CRISPR activation or repression experiments use targets...enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity...containing gRNA sequences designed to be used with any CRISPR nuclease and function. Please see the publication...

  11. CRISPR Plasmids - dCas9-FokI

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts A...FokI CRISPR: dCas9-FokI Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...off-target effects of CRISPR. Browse, sort, or search the tables below for CRISPR-FokI plasmids. Plasmids...CRISPR plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs). CRISPR... CRISPR dCas9-FokI ...

  12. Zhang Lab CRISPR Page

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    ... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang...Zhang Lab CRISPR Plasmids Available from Addgene Zhang Lab CRISPR Resources Tips for and FAQs about the ...Zhang Lab CRISPRs Browse All Zhang Lab Plasmids Zhang Lab Website You may also like... CRISPR Guide CRISPR...phages and plasmids. CRISPR loci in microbial hosts contain a combination of CRISPR-associated (Cas) genes...specificity of the CRISPR-mediated nucleic acid cleavage. We have harnessed the type II CRISPR nuclease system...backbone cloning information: CRISPR-Cas9 mouse toolbox protocol 637.8 KB CRISPR-Cas9 Cre expression vectors...CRISPR Cas9 gRNA plasmids from the Zhang lab Genome...

  13. CRISPR Plasmids - Single-Strand Break (Nick)

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    ...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR/Cas... CRISPR Nick CRISPR Plasmids: Nick...CRISPR nickase plasmids for the generation of single-strand breaks. CRISPR...Nick Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation...enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity... . Browse, sort, or search the tables below for CRISPR nickase plasmids. Plasmids are available for expression...

  14. CRISPR Plasmids - RNA Targeting

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type...CRISPR RNA Targeting CRISPR Plasmids: RNA Targeting Browse CRISPR Plasmids By Function Genome Editing Cut ...CRISPR plasmids for targeting RNA sequences, which include the enzymes C2c2/Cas13a and Cas13b. CRISPR... Type VI CRISPR systems, including the enzymes Cas13a/C2c2 and Cas13b, target RNA rather than DNA. In ... of Cas enzymes? CasPEDIA is an encyclopedia of CRISPR systems with wiki entries describing enzyme activity... . Browse, sort, or search the tables below for CRISPR RNA targeting plasmids. Plasmids are available ...

  15. Plan Your Experiment

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    ... Science Guides CRISPR Guide Plan Your Experiment Plan Your CRISPR Experiment Jump To: Plan Your Experiment...you started using CRISPR in your research. Although we will use the example of CRISPR/Cas9 in mammalian...express the CRISPR machinery. For more difficult cells (e.g. primary cells), viral delivery of CRISPR reagents...conducting genome-wide screens using CRISPR AAV transduction CRISPR elements are inserted into an AAV transfer...lipids Transient expression of CRISPR components Short window of CRISPR activity may decrease off-target... Cas9 Validate Results Publications Get Started CRISPR is a powerful system that enables researchers to... cells, many of these principles apply to using CRISPR in other organisms. First, consider the genetic...

  16. CRISPR Plasmids for Genomic Visualization

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Using... CRISPR Visualize CRISPR Plasmids:...CRISPR/Cas plasmids for labeling and visualization of genomic loci. CRISPR...Plasmids: Visualize Genomic Loci Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit...fluorescent proteins to visualize targeted genomic loci. CRISPR imaging has numerous advantages over other imaging...like fluorescence in situ hybridization (FISH), CRISPR imaging offers a unique method for detecting the...chromatin dynamics in living cells. The fluorescent CRISPR system has been used for dynamic tracking of repetitive...

  17. CRISPR-Cas/RGN expression plasmids for human cells

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    ... CRISPR-Cas/RGN expression plasmids for human cells You may also like... CRISPR Guide CRISPR Protocols... CRISPR Joung Lab CRISPR Plasmids Human CRISPR-Cas...gRNA Design Tools CRISPR Blog Posts This page lists plasmids required to practice CRISPR-Cas/RNA-Guided ...Joung lab CRISPR-Cas/RGN expression plasmids for human cells CRISPR...zifit.partners.org/ZiFiT/ChoiceMenu.aspx by choosing the “CRISPR/Cas: Design oligos for making guide RNAs” link ...

  18. CRISPR-Cas/RGN expression plasmids for Zebrafish

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    Collection
    ...Zebrafish CRISPR-Cas/RGN expression plasmids for Zebrafish You may also like... CRISPR Guide CRISPR Protocols... CRISPR Joung Lab CRISPR Plasmids Zebrafish...gRNA Design Tools CRISPR Blog Posts This page lists plasmids required to practice CRISPR-Cas/RNA-Guided ...Joung lab CRISPR-Cas/RGN expression plasmids for Zebrafish CRISPR...zifit.partners.org/ZiFiT/ChoiceMenu.aspx by choosing the “CRISPR/Cas: Design oligos for making guide RNAs” link ...

  19. CRISPR Plasmids - Zebrafish

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... CRISPR Zebrafish CRISPR Plasmids: Zebrafish...following CRISPR plasmids have been designed for use in zebrafish. Cut Fully functional CRISPR/Cas enzymes...CRISPR plasmids that have been designed for use in zebrafish. CRISPR...Zebrafish Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...selectable marker or cloning method. When using CRISPR, you will need to express both a Cas protein and...

  20. Boxem Lab CRISPR Plasmids

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    ...Addgene You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts We adapted the... CRISPR Boxem Lab CRISPR Plasmids Boxem Lab CRISPR...CRISPR Cas9 gRNA plasmids from Dr. Mike Boxem's lab. CRISPR... the S. pyogenes CRISPR/Cas9 system for use in C. elegans . We provide four different vectors for expression...digested vector. These plasmids are described in: CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis ...promotor. 47943 pMB70 : Can be used to express a CRISPR guide RNA in C. elegans from U6 regulatory sequences...
Showing: 241 - 260 of 446 results