...coli: purifying from Bacillus subtilis and Pichia pastoris pCri also includes plasmids designed for protein...bacterium Bacillus subtilis and the yeast Pichia pastoris. The improved secretory capacities of these microbes...producing difficult-to-fold proteins. In addition, P. pastoris has the capacity to glycosylate proteins and may...

... can edit the documents and track the revision history. Now you’ll be able to make updates to protocols...consider moving your paper spreadsheets for reagent storage locations, such as the list used to organize your...samples. Once you've taken some time to get organized, stop back and let us know what worked for you. Share ...

...was immediately interested in applying. This had stood out to me because it gave me a full description ...clear that Addgene wanted to ensure applicants understood what the expectations were for the intended position...make sure people feel connected.    As I became accustomed to the routine, I realized that the “9-5” was...

...postdoc-driven Future of Research Conference at Boston University last week.  This fantastic city-wide... supporters from all of the research centers in Boston.  I was relieved because it finally feels to me...program.  His strongest comments were about the astounding lack of reproducibility being recognized in published...

...should be able to tell your story for you and they should also be easily understood and consistent in design...Writing and Publishing Institute. Writing is the cornerstone of any scientist’s career. We use writing to ...

...protein (LanYFP) from the marine invertebrate Branchiostoma lanceolatum in 2012. Using structural modeling...applications from traditional imaging to FRET and stochastic single-molecule super resolution imaging. mNeonGreen...monomeric green fluorescent protein derived from Branchiostoma lanceolatum." Nature methods 10.5 (2013): 407...

...says. Percent sequence identity was a different story - the team found that the percent sequence identity...Addgene as a large data set. Alec A. K. Nielsen & Christopher A. Voigt also recently published a tool (trained...PMCID: PMC6200774.   2. Nielsen, Alec AK, and Christopher A. Voigt. "Deep learning to predict the lab-of-origin...

...sequences from a shared database of custom common features. Second, customers have asked for a way to annotate... involves iterative refinements in response to customer feedback. An example of this approach is SnapGene...

...of Dr. Elke Mühlberger and Dr. Rachel Fearns at Boston University School of Medicine, this post describes..., and a modified gRNA to direct a variety of customizable functions to your DNA sequence of interest. ... how the Casilio system works and how you can customize it for your experimental needs. Note: Casilio ...

...sequence of a mCherry fluorescent protein followed by a stop codon and the coding sequence for a green fluorescent...success of the change in expression of GFP, for the stop codon should have changed from “TGA” to “TGG.”  ...acceptor and donor sites, and introducing premature stop codons.    New from the viral service By Jason...

...to argue otherwise). To be sure, throughout the history of medical research, there have always been people...CRISPR/Cas9 research” but whether or not we will stop shuffling our feet on necessary actions to review...the future of genome editing technology. We must stop punting decisions on scientific, ethical, regulatory...

...quick turn-around time of Sanger sequencing have historically matched the needs of most molecular biology ...displayed at the individual read level, and because custom primers or "primer-walking" reactions are completely...problems. He received his PhD in Bioinformatics from Boston University, and did postdoctoral work at Harvard...

...enormous lattice of identical subunits. A brief history of R bodies R bodies have been studied for many...recombinant E. coli on paramecia. European Journal of Protistology 48, 290–296 (2012). PubMed PMID: 22356923. 3... Polka, J. K. & Silver, P. A. A Tunable Protein Piston That Breaks Membranes to Release Encapsulated Cargo...

...Addgene has developed in terms of the collection, storage, and distribution of the plasmids and their ability... Michigan, Ann Arbor and obtained her J.D. from Boston University School of Law, where she was on law ... of Advisors and Audit Committee at the Greater Boston Food Bank and participates locally in Read To A...

...design a custom primer. Because Sanger sequencing can usually sequence just 1 kb of DNA, custom primers...All_in_one_CRISPR/Cas9_LacZ, a CRISPR gRNA plasmid from Lynne Postovit’s lab. It contains an ampicillin resistance marker...

...assembled a cross-team team, with members from the Customer Service, Data Curation, Outreach, Product, Scientist...particular and our deep understanding of Addgene’s customer’s needs. Categories We created a categorization...of our information in order to more granularly customize the search engine, and a scoping mechanism that...

...them.   When you think about it, virtually every milestone in a scientist's career is mediated by some kind...flagship workshop.  Our 2018 workshop took place in Boston - you can revisit the activities of our attendees...careers in science communication The strength of story telling Science communication: 9 strategies to get...

...RNA-editing Cas13 enzymes have taken the CRISPR world by storm. Like RNA interference, these enzymes can knock ...from this paper are available at Addgene. Yan, Winston X., et al. “Cas13d Is a Compact RNA-Targeting Type...Plasmids Check out our CRISPR Guide Read our CRISPR History  ...

... addition, there was an entire story-telling session organized by Story Collider and which featured a ...the different parts of the brain stimulated by stories. In retrospect, I should have expected this given...

...in C++ and Java Command line history: Tracking command line history by recording all commands entered...addition to output files, this is also where you can store any power point, excel, or other files relevant ...