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We narrowed to 353 results for: SAM activators

Showing: 241 - 260 of 353 results

  1. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    ...commission in the United States Air Force. She left active duty to pursue a degree in a synthetic biology ... synthetic biology lab Keira and I worked in the same lab for four years. It was a cutting-edge synthetic... on the detector plants, we expected to see that same anger. Instead, social media comments were more ...

  2. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... reporter plasmid with very low basal activity shRNA/RNAi Gene silencing Browse RNAi empty vectors... Mammalian pLX304 - 3rd gen lentiviral vector lentiSAMv2 - Lentiviral sgRNA cloning backbone pLKO.1-blast...

  3. Antibodies 101: Conjugation

    Type
    Blog Post
    ...each of these enzymes allow you to detect enzyme activity (and therefore your antibody) either through a... antibody will have been verified to perform the same as its unconjugated version, but if you find yourself...

  4. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ... include tetracycline or doxycycline mediated activation of Cre to either induce or repress Cre expression...repair is a random process, zygotes edited using the same nuclease may not result in genetically identical...

  5. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    ... Doench CRISPR-Cas9: Tips for Optimizing sgRNA Activity by John Doench All CRISPR Posts  ...Beatty et al., 2012). These features make AdV an attractive candidate for in vivo delivery of advanced genome...achieved by isogenic AdVs encoding TALENs targeting the same chromosomal region. The CRISPR/Cas9-derived RNA-...in a second paper published in Nature Methods the same year that delivering RNA-guided nucleases or TALENs...

  6. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...Beyond simply monitoring protein localization and activity, CRISPR tagging systems can be modified to assay... 2-3). Since donor plasmids of the same type will produce the same unique SapI 5’ overhang, different ...about Fluorescent Protein Timers Check out Photoactivatable Fluorescent Proteins Use Rosella to Monitor...

  7. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...proteins that target specific tissues are areas of active study (for a review click here). Alternative envelopes...often: Q1: Are retroviruses and lentiviruses the same thing? Can I package lentivirus with retroviral ...both lentiviruses and gamma-retroviruses use the same genes for packaging (that's gag, pol, and env), ...initial infection. SIN is shorthand for self-inactivating, which is achieved by deleting a large portion...

  8. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ... one’s work. As a community, we must therefore actively work to remove the stigma around sharing negative...well for a neighboring cell type, even within the same brain region or nucleus. Just optimizing AAV expression...vectors, many from people trying to answer the exact same question I was: What AAV vector is best for my specific...wanted to see more negative data (Figure 2.) At the same time, when scientists who support open science, ...

  9. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...lines in which each cell has the capacity to be activated to produce infectious murine leukemia virus. Virology...instructions in the same producer cells as the virus. Because it is made at the same time as the other ... a retroviral particle, which is essentially the same for viruses and viral vectors. Key to the wild-type...

  10. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ...one area at a time and try to say “no” to other activities. Take care of yourself. Take a vacation alone...whatever works for you, but most days go home at the same time. Use time management tools and tactics to plan...need to match up exactly when you fold them. The same with sheets – wash them and put them right back ...family by forming a social circle of people in the same situation. Go to church, join a club, meet the neighbors...

  11. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ... reciprocal, forming a neuronal loop which is activated and strengthened during memory formation and memory...connections that are functionally relevant, i.e. activity-dependent neuronal tracing. Apart from mapping...al., 2008) AAV6 (Towne et al., 2010), AAV8, -9 (Masamizu et al., 2011); AAV5 (Aschauer et al., 2013). Anterograde...PMCID: PMC5529245. Choi, V.W., McCarty, D.M., and Samulski, R.J. (2005). AAV Hybrid Serotypes: Improved Vectors...PMC2895694. Hollis, E. R. 2nd, Kadoya, K., Hirsch, M., Samulski, R. J., and Tuszynski, M. H. (2008). Efficient...PMID: 25374356. PubMed Central PMCID: PMC4269290. Masamizu, Y., Okada, T., Kawasaki, K., Ishibashi, H., Yuasa...PubMed Central PMCID: PMC5572074. Salegio, E.A., Samaranch, L., Kells, A.P., Mittermeyer, G., San Sebastian...

  12. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...system incorporates the Tet-off transcriptional activation system to drive high levels of expression of ... 10 cm2 dishes. While this option is attractive because the same procedure for a single 10 cm2 dish can...systems such that it no longer requires the viral transactivator, Tat. For more information on the different...

  13. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...host chromosomes, further minimizing potential activation of innate immunity. AAV vectors are also capable... PubMed Central PMCID: PMC3152950. 3. Jacobson, Samuel G., et al. "Improvement and decline in vision with...

  14. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...wide variety of screens, including interference, activation, and knockout. Most CRISPR screens rely on ...expression of lentiviral vectors make them an attractive option. However, safety requirements and expression...vivo. The general process of these screens is the same: Deliver CRISPR library to cells of interest. Select...Low, S. H., François, A., Suárez-Amarán, L., & Samulski, R. J. (2022). AAV vectors: The Rubik’s cube of...

  15. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...back, better and safer. It is now one of the most active and exciting areas of science. This is hopefully...retinal cells - AAV - were becoming available. At the same time advances in human genetics led to the identification...24664759. PubMed Central PMCID: PMC4267879. 10. Samulski RJ, Muzyczka N., 2014. AAV-Mediated Gene Therapy...

  16. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...State University Use Nanobodies to Control Protein Activity Learn more about protein tags Read about E. coli...figures 4A-C). And since they have limited cross-reactivity between different IgG subclasses and different...primary antibody-nanobody mix can be added to your sample, thus eliminating the secondary antibody incubation...568, or Alexa Fluor 647 before application to the sample. Staining with this multi-color staining workflow...

  17. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...used to measure conformational changes, cleavage activity using FRET-based biosensors, and interactions ...What is FRET? In their JCB 2003 paper, Sekar and Periasamy defined FRET as "a distance-dependent physical...341-50. doi: 10.1002/bies.201100098. Sekar RB, Periasamy A. Fluorescence resonance energy transfer (FRET...

  18. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ... dendritic cell function and increased B cell activation. PLOS ONE, 12, e0180664. https://doi.org/10.1371... are represented as a series of dot plots in the same order. However, in an actual flow cytometry run,...parallel.  Let’s imagine we’re looking for cells in a sample that are negative for marker A and positive for... proxy for nucleic complexity of the cell.  The sample will have been labeled using a panel of markers... dots. In a contour plot, each line contains the same number of events. Thus, contour lines that are close...

  19. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...the nucleus where they exert their recombinase activity (Logie and Stewart 1995; Metzger et al. 1995)....reintroduction, inversion and re-inversion happen at the same probability. Continuous inversion of the DNA sequence...recombination can only occur between target sites of the same sequence in FLEx vectors, the DNA sequence that ...manipulations in vivo, FLEx vectors expressing the light activatable ion channel under the control of Cre recombinase...neuron and its synapse should be visualized at the same time from two different plasmids this approach becomes...connectivity before and after the gene knockout in the same mouse.   Scientists always strive for more....
Showing: 241 - 260 of 353 results