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  1. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ... at Addgene, ComSciCon, and many more great organizations. At ComSciCon, our primary mission is to strengthen...respective fields. To help attendees launch new organizations that will extend opportunities to practice science... create them.  Seek out collaborations with organizations that practice science communication or start...
  2. Hot Plasmids - May 2022

    Type
    Blog Post
    ...proteomic mapping in both cell culture and whole organisms (see our 2019 overview). Until recently, the approach...dedicated transgenic line. Using zebrafish as a model organism, the Parton Lab recently created a flexible system...Given that the liver is an immunologically active organ, this advance is likely to afford the ability to...
  3. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ...stakeholders, the product managers collate and organize that information and form it into a roadmap--a... features, programs, or tools to build. After organizing the information, a software product manager works...everything running smoothly, you’ll want to be really organized. Often, product managers will have three or more...
  4. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    ... work with Drosophila as an experimental model organism. I then described the Gal4/UAS system used by ... survival. For those interested in development, organs of interest, such as the intestines, can be dissected...have!) (4). The power of Drosophila as a model organism is in its amenability to genetic manipulation....
  5. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...engineer a specific function in these multicellular organisms. However, the rewards are great if you take the...are needed to increase the body of knowledge. Organizations like Open Plant can then build on this research...plant circadian clock. Petunias have been a model organism for plant research for decades, and the biochemical...
  6. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...defined as the inability of an organism to synthesize a particular organic compound required for its growth...DNA transformation system.Unlike most other microorganisms, yeast have both a stable haploid and diploid...
  7. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

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    Blog Post
    ...like to start a group mentoring program at your organization, contact me! I can provide a step-by-step, how-to... people from different labs, departments or organizations as it brings a diversity of perspective. Sometimes...transition. Schedule your first meeting and get organized. Discuss your general areas of interest and what...
  8. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...lentiCRISPR v2-sgCOQ2 COQ2 U6 Multiple system atrophy Boyi Gan 186221 pBMN BFP-Parkin PRKN BFP Parkinson's Michael...Frontotemporal dementia in GRN mutation carriers Michele Pagano 210366 TMEM106B-EGFP TMEM106B GFP CMV, T7 Frontotemporal...Frontotemporal dementia in GRN mutation carriers Michele Pagano 210899 pFBOH-MHL_LRRK2:2141-2527 LRRK2 His polH...
  9. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...More…” menu. You can also follow companies and organizations to see their news and job postings. 4) Use ...connections can connect you to people at that organization. This type of connection can help you gather...that is not relevant to business or volunteer organizational work. LinkedIn is not the platform for politics...
  10. dTAG - You're it!

    Type
    Blog Post
    ...conjugating a CRBN-binding ligand (thalidomide) to an FKBP12F36V binding ligand (ortho-AP1867) via various...molecule is dTAG-13 due its immediate action, potent ligand performance, great depth of degradation, absolute...
  11. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...celebrate! As we enter this new growth phase of the organization and the expansion of our impact, I’d like to...service and contribution! Leadership On an organizational level, we keep passing our audits with flying...even via Twitter. We love working with local organizations like Just-a-Start, the Northeastern co-op program...
  12. PITChing MMEJ as an Alternative Route for Gene Editing

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    Blog Post
    ...of NHEJ, HDR, and MMEJ repair varies from organism to organism. MMEJ doesn’t yield a “perfect repair” like...researchers can exploit for CRISPR gene editing. In organisms where HDR is downregulated, MMEJ represents another...
  13. CRISPR in the Clinic

    Type
    Blog Post
    ...functional correction of the gene in clonally expanded organoids. This work, and many others, pushed the field ... post was originally written in 2014 by Kendall Morgan and updated in 2023 by Susanna Stroik.  References...of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients." Cell Stem Cell. ...
  14. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...concentrations and transients in living cells and organisms. Unlike chemical indicators, these DNA constructs...constructs can be delivered to intact tissues and organs noninvasively and target a defined population of cells...Genetically encoded fluorescent indicators for organellar calcium imaging. Biophysical journal. 2016 Sep...
  15. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...tech transfer team will communicate with your organization’s technology transfer office or other authorized.... rerio (zebrafish), D. melanogaster (fly), C. elegans (nematode), S. cerevisiae (budding yeast), S. pombe.... The name of the Principal Investigator and Organization where your plasmids were first created. From...
  16. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...generated using random genomic fragments from the organism of interest, or a defined cDNA library (e.g. the...number of two-hybrid systems available in model organisms, including bacteria, alternative fungi (C. albicans...Enzymol. 1983;100:293-308. PubMed PMID: 6312261. 2. Keegan, L., Gill, G., and Ptashne, M. Separation of DNA...
  17. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...Chromatography columns containing appropriate immobilized ligands are incubated with cell culture supernatant containing...antibodies. The antibodies bind the immobilized ligands and can be eluted once the other materials have...incubated with the cell suspension. Antibodies bind ligands on the beads and a magnet is used to separate the...
Showing: 241 - 260 of 601 results