...collate some of the current “best” FPs, and I collaborated with him on a web-based interactive visualization...deal more information that one might like to store about FPs than is currently represented at fpvis (complete...thousands of scientists routinely using and thinking about FPs, it would be great to have a centralized, community-editable...resource for storage and dissemination of information about FPs. FPbase.org FPbase is a project written in ...comprehensive community resource for information about fluorescent proteins.  It was designed with the ... there is plenty of room for improvement and elaboration.  Current projects include incorporation of biosensors...protein evolution lineages, 2-photon spectra, more elaborate spectrum analysis tools, and a better way to store...

...manipulation more hands on While teaching courses about gene therapy and genetic manipulation, I and other...with some other professors, I went home thinking about how to solve this problem. I didn’t want to face...therapeutic strategies work. Find Addgene Blog posts about other educational tools Playing BUILD YOUR PLASMID...winner? Ultimately, society should be! This is a collaborative game, as it should be in real scientific practice...practice transferable skills like team building, collaborative critical thinking, and communication among others.... My impression was that they had clearer ideas about gene therapy in the following lessons. And let me...biology Learn how BioBuilder can help teach students about Synthetic Biology Find Arabidopsis educational resources...

...each of your meeting types (see above). We’ve provided some tips above for what works well at Addgene,...opposite effect. A great meeting should produce collaboration - a sense of dialogue and community among participants...meeting to discuss something difficult, be excited about the prospect of working toward a solution. At the...sections for each person to add a bullet or two about what they are working on. Building this into the...purpose of the meeting (see “defining the scope” above) or if you can cut it down to thirty minutes. ...some tips. Before long, you will be running collaborative, informative, energizing meetings all your own...

...start their own academic research laboratory. How does one even go about starting a lab? Obviously, funding...always helps. But how does one build a strong collaborative research team motivated by similar visions and...booklet. What has been the most surprising thing about becoming a PI? Tom Ellis has a one word answer: ... has to deal with. My inbox currently stands at about 65,000 messages and dealing with email can be half...the first couple of hires to build a strong, collaborative team. And take advantage of the many resources...Medical School) for taking the time to speak with us about adding new scientists to their labs. Further Reading...

...sponsored the capital construction of our new BL2+ laboratory and the infrastructure capabilities to produce...our generous, sharing depositors. Over 3,000 laboratories are sharing plasmids (with 16 labs currently...helping thousands of sharing scientists from laboratories all over the world. With this reach and exposure...In early 2016 we surveyed hundreds of scientists about various ways we might expand Addgene’s services....provides viral vector technical service (details about virus production protocols and quality control can...material. The Outreach Team is getting the word out about this new resource and providing fantastically useful...Outreach Team has been making sure scientists know about the new initiative and serving all the new educational...

...You never know when you will meet a potential collaborator Some of these people you meet will review your...know personally Scientists in your field will know about grants and projects that might help your work Tenure... your card, they have to give you theirs! Think about how you want to introduce yourself and what you ...those you would like to try and meet. Don’t worry about contacting someone much more senior than you – meeting...questions you are pondering and be ready to talk about them. People love to help especially when solving...“Is this seat taken? Can I join you?” Next talk about the food and then move on to science. More great...conference meal etiquette are here. Don’t only talk about science and work – casual socializing is how people...

... related blog posts Learn about our cancer collection pages Read more about the tumor microenvironment...the tumor. How do microenvironment enzymes and metabolic reprograming influence tumors development? Being... vice versa, let’s take a look at cancer cell metabolism. Cancer cells promote oxidative stress leading...of cells affect tumor progression, enzymes and metabolism reprogramming aren’t the whole story. It’s essential...particularly interested in science journalism and writing about biomedical issues. Follow her on Twitter @BrbaraPinho6...resources page  Find plasmids for your research Learn about Addgene's viral service ...

...and gives you a sense of what people are excited about. Following scientists in fields outside your main...research organizations, and universities often tweet about publications in the fields of research they support...journals: They publish papers, so naturally they tweet about papers too. You can follow journals related to your...field of research or larger journals that publish about a broad range of topics.  Twitter paper bots: Twitter...number of retweets, likes, and comments on a tweet about a paper can help you gauge how “hot” a paper is....papers that the scientific community is talking about and jumping into the discussion! Semantic Scholar...and search for papers later. Pro tip Learn more about authors by exploring author pages (for example, ...

...resources on the Addgene blog Learn more about SapTrap Read about CRISPR/Cas9 in C. elegans Browse our Plasmids...always emphasized the need for open science and collaboration. The field already has comprehensive reference...planned for August 15th, 2019. For more information about the aims and approach of CeNGEN, check out their...elegans. This database provides detailed information about C. elegans 3’UTRs structures, alternative polyadenylation...addition to the wealth of open resources described above, there were many plasmid tools presented at the ...by the worm community. For additional resources about C. elegans and the plasmids we have at Addgene check...

...heard about it, I got excited from a computer scientist perspective. But, the more I thought about it, ...maintains a list of active spaces near you.   Learning about C. elegans with the OpenWorm Project One such space... an example of one of the problems we work collaboratively to solve, we want to focus our research on ...mutant known as CX51 that exhibits motor defects above 25 degrees. Computer scientists and biologists work... work together to compare the predictions about the effects this mutation has on locomotion in our computer...optimizing the health of domesticated cats and learning about feline microbiomes. One of the aspects of this project...

...spectrum is located in a range of wavelengths of about 380–700 nm. Photons of lower wavelengths are higher... 650 nm, APC-Cy7's global excitation maximum is about 750 nm. If we were to use a laser that excites at...optimal excitation happens at higher wavelengths of about 750 nm. Source: https://www.bdbiosciences.com/en-us... we start building your first panel, we'll talk about one last principle of color emission. As you can...our blog post on color compensation to learn more about them. Alright, let's build your first panel! Designing...Conclusion You made it! In this post, you learned about the principles behind fluorophores and their colors...this article again. In the beginning, we talked about the relationship between a photon's wavelength and...

...potential beneficiaries. Collaborative therapy For the most efficient outcomes, collaborative therapies must be... of the tolerance-producing antigens enunciated above. Finally, signals released from the microenvironment...are in their clinical trial phase. In all of the above therapeutics, techniques like genome engineering...are beginning to see the fruits of many years of labor with up and coming immunotherapy treatments. As ...clinic, physicians and researchers are learning much about how treatment regimens can be improved and how the...ahead of the disease in patients and developing collaborative treatments will go a long way in combating this...Additional Resources on the Addgene Blog Learn About Our Cancer Collection Pages Plasmid Grant to Aid...

... electrophysiology data too but that’s what collaborators are for). For example, if you study neural pathways...intended brain region and/or cell type (I’ll talk more about this in detail later). But what are the different...of your opsin, but keep you eyes open for posts about the next steps in the coming weeks. Expression of...Addgene. See the Deisseroth lab homepage for details about types of constructs available, instructions for ...1, 2, 4]. And, of course, the best way to learn about the many different opsin variants and how they can...point.  In brief, some standard opsins (see table above) include Channelrhodopsin2 (Chr2), a blue-light ...construct. Stereotaxic surgery  As mentioned above, AAV opsin constructs are injected directly into...

... elevator pitch slightly if you know a bit more about the other person. If you have a poster or talk at...seemed confused, bored, or particularly excited about? If you tried tweaking your pitch throughout the...conference If there’s a cool new method that you learned about at the talk, bring it to your next lab meeting. ...questions. If you or your lab identifies a potential collaborator from the meeting, reach out to them, pointing...their talk at the meeting and why you think a collaboration would be beneficial. The poster session Before...conference. Networking All of the things we've mentioned above could be considered networking, but here are some...you remember who they are and what you’ve talked about. After the conference Follow up with those that ...

...larval stage, Drosophila will pupate, which takes about one week from the initial deposition of the fertilized...there are many resources to help you learn more about fly biology and fly-based experimental systems. ...resources will direct you to the latest information about gene function, while others offer fly stocks and...and other reagents. If you’re ever confused about the best way to study YFG in flies, there is likely an...absolutely essential. You can look up information about your fly gene using the search box in the upper ...In the next post, you’ll learn the fundamentals about how many of these resources work. Many thanks to...Site Specific Recombination in Drosophila Learn about Cre-Lox Resources on Addgene.org Find Drosophila...

...Foundation's Linda Kahl explains the Addgene collaboration this way: "Addgene is one of the leaders in ... our core values." Addgene spoke with Endy more about the BIOFAB and BIL gates plasmid kits, the Biobricks...reference to George Boole who in 1854 was thinking about logic and analyzing human language and observed ... freely available? Endy: When we start thinking about languages, humans have two types of languages: one...does; it's free to use and that tends to be true about most successful human-to-human languages. Who owns...signals. Let's say I wanted to detect every primary metabolite in a cell. The only way to do that at scale right... what can you use biology to make? If you think about the BIOFAB collection and really dig into its engineering...

...share. I spend a lot of time telling scientists about Addgene’s repository, and explaining how to deposit..., lab managers, and grad students. We’ve talked about their hot-off-the-presses papers, the awesome new... learned along the way that a connected and collaborative community makes not just for a better day at...technologies and breakthrough research. Insights about how to navigate through all stages of your career...cloning and plasmid-based technologies. Commentary about scientific sharing and the open-access movement....So join the conversation! Do you have a question about culturing iPS cells? Confused by which system to...

...have been written about CRISPR alone, and Zimmer himself has written entire books about optogenetics. Defining... been thinking about a long time. For the scientists who like to learn everything about one specific topic...wisely makes no such attempt. He is transparent about his own biases--many of which will be shared by ... a massive, expensive project that required collaboration from scientists around the world. The paper ...As a scientist who enjoys learning a little bit about many different things (part of the reason Addgene...

...SLIC cloning Learn about Gibson Assembly Additional Resources on Addgene.org Read about Choosing a Molecular...this type of primer does not provide information about the orientation of the insert. Orientation-specific...Orientation-specific primers: If you need information about insert orientation, then you might consider designing orientation-specific...insert Backbone-Specific Provides information about the size of the insert and if it's in the plasmid...plasmid dependent Doesn't provide information about the orientation of the insert Orientation-Specific... before pipetting them onto the gel. The figure above summarizes generalized expected results for the ...

...common cancers like lung (about 224,000 diagnosed this year) or breast cancer (about 246,000 diagnosed this...Mesothelioma Cancer Alliance. We have learned much about the causes of cancer and the different avenues that... asbestos exposure to the time of diagnosis) is about 35 years, but it usually ranges from 20-50 years... an orphan cancer; it is rare disease with only about 3,000 new mesothelioma patients diagnosed every ...from mesothelioma several years ago – recently collaborated with Senator Barbara Boxer to introduce the ...Additional Resources on the Addgene Blog Find out More About Cancer and Addgene's Cancer Collection Pages Learn...