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Showing: 221 - 240 of 1045 results

  1. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ... an antibiotic resistance gene to allow for antibiotic selection, but these resistance systems don’t always... develop resistance to selection toxins. Auxotrophic selection is useful for producing stable mammalian...of bacterial transformation is transfection. In stable transfection, DNA from the plasmid is incorporated...transfection, the vector remains episomal, meaning it stays separate from the genome. One option for episomal...Mammalian cells At least one of the following: to stably transfect the vector a viral ori in a compatible...shared aspect of ribosomal function. A puromycin resistance gene on your vector can protect both plasmid-...also block protein synthesis, and the neomycin resistance gene protects against any of them. But different...

  2. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...always a good idea to start close to your comfort zone and slowly expand from there. Start small: Collaboration...implementation, and now must focus on managing the established partnership. If you’re willing to do similar ...my graduate studies at Karolinska Institutet I started working with other groups. First within my department...example, I worked with some microscopy enthusiasts to start a microscopy seminar to connect the imaging community... scientific collaboration - show initiative and start organizing meetings and take minutes. Get to know... little in common with you is much harder than starting a collaboration with a colleague. However, reaching...discussing with strangers stopped feeling weird and started to be fun. I just had to remember to approach the...

  3. Finding Your Science Policy Path

    Type
    Blog Post
    ...high school to college to graduate school, standing at the start of a science policy career was much more...for science policy folks is on Twitter- start an account and start engaging with the #scipol hashtag. Often....  Who does science policy? I’ve intentionally started with the ‘who’ question because the science policy... from your alumni community is a great place to start. What is science policy? THE IMPOSSIBLE QUESTION...branch or in Congress, or can also happen at the state and local level. The big secret about science policy...tenured professors. There’s never a bad time to start your science policy journey.  In fact, working in...your career through engagement with your local or state governments. Most communities have opportunities...

  4. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...biology, innovative new imaging approaches are constantly being developed which combine computational processing...this. This recent push gives users the chance to understand the "black box" provided by the manufacturers.... 1: Open source hardware builds   Since I started my PhD, I have been convinced that most microscopy...creativity and feedback from the community to create state-of-the-art devices for research. There are a number...support the community in developing better, more sustainable, easily sharable products. Open-Source Microscopy...time-lapse images, quantitatively evaluating antibody staining, or counting cells are now routine, open-source... process framework FIJI are just three of the constantly growing number of "community-driven" software...

  5. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...Challenge #2: Technical obstacles to plant synthetic biology In addition to public resistance, an overall limited...limited understanding of plant biology is a significant technical obstacle to plant synthetic biology. We.... Read their previous blog post about how they started their company here. The first transgenic plant ...technology. Monoculture and chemical spraying, mainstays of modern agriculture, are now synonymous with...imagine any use for GM technology other than to sustain those techniques. We feel strongly that the scientific... We cannot build what we do not understand, and the complicated and unique metabolic processes of plants...project in a field with 30 years of basic research standing behind it. Our color changing flower involves ...

  6. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...bind DFHBI, is highly unstable in vivo due to poor folding and thermal instability at 37°C (Ouellet et ...to the instability of Spinach, and were able to develop an aptamer that was more thermally stable, exhibited... not photostable, and were presented in the study to support the uniqueness of the photostability of the...) and requires a tRNA scaffold to improve its stability in vivo. However, Spinach has proven useful in... RNA. Spinach-based riboswitches can be used as stable metabolite sensors for imaging in live cells. By... fluorescence. In the search for something more stable for quantitative imaging, Ming Hammond’s group ...lower dependency on magnesium and higher thermal stability, making it a potentially more useful tool for ...

  7. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ...imaging is an easy way to understand what is going on in the body at steady state or under physio-pathological...adult organisms, where tissues structures are more stable and local movements predominate (e.g. a basal progenitor...progenitor cell migrating to the epithelium). At this stage, only cells from the immune system show wide scale...information regarding immune cell mobility than the static microscopy images of chemically coloured lymphoid...immunologists the ability to count cells and statistically analyze cell movements. By using cells labelled...two-photon microscopy allowed scientists to better understand T cell initiation in the adaptive immune response...uncovered host-pathogen interactions leading to the understanding of effector function in infected tissues. Before...

  8. How to Prepare for an Industry Interview

    Type
    Blog Post
    ... The STAR interview method is a popular way to answer behavioral interview questions. STAR stands for ... describe all four parts of a STAR answer once the interview has started. Instead, I preferred to prepare...was my experience not long ago. I just recently started working in the pharmaceuticals industry after earning...several individual and/or small groups, usually starting with the hiring manager. These interviews lasted...Story telling shares many of the same elements as a STAR answer: a setting, a conflict, and a resolution....which is something not inherently captured by the STAR method. I like to think of the theme of my interview...resumes are like cliff notes, so I use these as a starting place for drafting my stories. In total, I prepare...

  9. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...antibody to stain a target protein in mouse tissues, causes a high degree of background staining. This occurs...secondary antibody) approach?  Staining for multiple targets When staining multiple targets indirectly, ...for a four-antibody panel, staining in parallel    In short, when staining multiple targets, look for...validated for your application, of course!) When the stars are aligned and the lab gods are smiling down at...the tissue sample. There are species-on-species staining protocols that use antibody fragments to block...endogenous immunoglobulins and reduce the background staining but they do not always eliminate the issue (Lu...better to choose primary antibodies from more distantly related species and/or use narrowly specific secondary...

  10. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    ...for you, don’t wait. Start communicating now. These 9 strategies will get you started:  Subscribe to Career...Take the DIY approach and start a blog. Stellar talents like Ed Yong jumpstarted their science writing career...Twitter account. Check out the hashtag #scicomm and start following science communication societies like @...local pub at a Pint of Science event. Or get on stage for a Science Slam – a sort of competitive TED talk..., set up a blog using a site like WordPress and start writing. Grow your audience by advertising your ...are open to guest contributions. If you decide to start your own blog, check out these two excellent articles... is the path you want to follow, check out the distance learning courses at the National Council for the...

  11. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...to gain support for CINSO. For instance, I tweeted to the Indiana State Museum and others when I was trying...engaging the public to promote an awareness and understanding of science. Like most science PhD programs, ...often called “nontraditional,” helped prepare me to start CINSO. Here, I share some of the tips I learned ...up and moved on to the next thing. If I made a mistake, I evaluated what lessons I had learned – call ...the process. As I mentioned, my graduate office staff was amazingly supportive. I talked to them about... had to take advantage of it. Blog and tweet I started my blog, Born to Science, as a graduate student...experience with my blog helped immensely when CINSO started brainstorming the resources we wanted to provide...

  12. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    ...Purdue University and is working to understand Lowe Syndrome, a devastating genetic disease caused by mutations...developing metastatic melanomas and other cancers. Joshua Wang (bottom right), also of the Wistar Institute... patients with the condition to reveal some “outstanding differences.” They now have some theories on ...behavior in greater detail. The goal is not just to understand the disease better but hopefully also to find...Rare Genomics Institute and Addgene will make a substantial difference in pushing the work forward. Xeroderma...Hendrik De Buhr (top right) and his colleagues at the Wistar Institute in Philadelphia, PA will put their award...the lab got interested in XP out of a desire to understand the early events in the transformation of healthy...

  13. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...control copy number and stability, which influence the plasmid’s ability to establish and maintain itself ...Understanding the characteristics of natural plasmids and how they function in nature can inform us on...others have a broad host range and can replicate and stably maintain the genes they carry among organisms belonging... many different phylogenetic groups (Jain and Srivastava 2013).  Recombinant vectors that are made from...from broad host range plasmids are more stable, can be easily cloned in different bacteria, and can be ...transferred to various experimental hosts (Jain and Srivastava 2013). They are also easily-shareable tools within...utilize diverse host replication machinery. For instance, the ori typically contains iterons that serve...

  14. How to Be an Excellent Trainee

    Type
    Blog Post
    ...rotation students, joining a new lab at any career stage, learning a new technique while visiting a lab, ...technical mentorship from a ‘bench mentor’. In some instances, the bench mentor is also the scientific mentor...mentor…but usually the bench mentor is a staff scientist, postdoc, lab manager, technician, or a graduate...there any relevant papers you can read prior to the start of your training? These could serve two purposes...and other educational resources to help you get started!) Are there protocols you can familiarize yourself...materials (maybe a step in the protocol you don’t understand the purpose of, or a different way of doing something...your bench mentor if they have time to answer outstanding questions you have, well before experimental ...

  15. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...schools and was started in 2005 in Kansas by Bobby Henderson, a physics graduate from Oregon State University...through synaptic connections (Shepherd, 2004). Understanding the structure and function of these neural circuits...researchers often want to do at neuronal synapses for instance. Also these tags need to be fused to scaffold ...scaffold proteins in order to be expressed stably in neurons. As compared to epitopes tags, FPs are intrinsically...circuits and dynamics. FPs are generally bright, stable and well tolerated by cells. They can be used for...The issue with these FPs is that they are less photostable than GFP. In addition, antibody-based amplification...useful for multicolor imaging experiments than standard FPs. The Looger Lab was thus able to easily perform...

  16. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ... its unbound state contains a fluorophore and a quencher in close proximity. For instance, the fluorophore...was less stable due its RNA nature, it paved the way to new biosensor possibilities. For instance, further...years ago, two independent groups, led by Jack Szostak and Larry Gold, developed methods for selecting... from experts on FRET techniques. By combining standard donor/acceptor FRET know-how with aptamers, aptabeacons...aptamer system. Knowing that GFP’s  structure stabilizes a 4-hydroxybenzylidene imidazolinone (HBI)-like...Spinach eventually evolved into Spinach2, a more stable and brighter version of the aptamer, but it still... of SELEX and FACS, Broccoli is inherently more stable in cells. The new aptamer also has a higher melting...

  17. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...regardless of visa or fellowship status, in addition to certain staff scientists and those in technical...$47,476 per year, under updates to the Fair Labor Standards Act (FLSA). This is having a major effect on ...students are exempted from the law. However postdocs, staff scientists and technicians in primarily bench-science...$43,692 to $50,000, an increase of 14.4%. In 2015, Stanford raised salary levels to $50,000, and as of October... The University of Notre Dame sets no minimum, stating that while they recommend NRSA levels, postdocs...not to be tracked. University of Buffalo and Ohio State both have minima of $30,000, and raising salaries...addition, both Brown and Brandeis universities have stated that they consider postdocs paid on stipends to...

  18. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    ...is also an avid long distance runner, gardner, and cook.      Lindsey M. Costantini received her PhD in...by guest bloggers Erik L. Snapp and Lindsey M. Costantini. "You underestimate the power of the Dark Side...non-cytosolic protein functions properly? We suggest starting by using the newly published oxidative optimized...used in various combinations. For example, with standard fluorescent microscope filter sets, users can ...when utilizing an optimized moxFP as opposed to standard FPs. The increase in signal is not due to a brighter...systems should strongly consider using moxFPs over standard FPs for the reasons listed above. In addition,...susceptible to the oligomerizing effects of many standard FPs. The moxFPs are now available through Addgene...

  19. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...method requires constant turbidity of the culture, so bacteria must be grown in a turbidostat. Only mutate...spectinomycin resistant phenotype in bacteria. While there are many known spectinomycin resistance mutations...only uncovered novel resistance genotypes, but also provide a better understanding of the key residues ...of wild-type E. coli, while only increasing the standard mutation rate seen during DNA replication by 120... than the original nCas9, while increasing the standard mutation rate by only ~2-fold over nCas9 levels...allow researchers to trace the progeny of a single starting cell. Additionally, multiplexing of guides allows... feature which they used to generate bacteria resistant to both spectinomycin and streptomycin, but could...

  20. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    ...grateful, I started setting aside 3-5 minutes to sit quietly at my bench before the start of any experiment... you’re much more likely to make a mistake. When you make a mistake, you’re more likely to be stressed...experiment failing because of a small, avoidable mistake. Or worse, when you're trapped in a pattern of ...same goes for your workflow. Next time you make a mistake, instead of silently vowing never to do that again...workflow, or your physical setup - to prevent the mistake, just like you would change variables to fix a ...a failed experiment.  For example, I used to constantly underestimate time needed to prep for an experiment...experiment. What fixed it was not trying even harder to start earlier, but simply moving all my prep work to the...
Showing: 221 - 240 of 1045 results