...) the courage to make mistakes and 2) the integrity to recognize these mistakes quickly. However, many...same atmospheric physics to understand exoplanets that we use to understand the Earth! Denver, CO, USA ...we, scientists and non-scientists alike, need to stay active and involved if we wish to make positive ...role of science in our democracies will require constant vigilance and actions beyond marches, as well ...Institute, most women of reproductive age live in a state with at least two abortion restrictions that conflict...not only believe in the separation of church and state, but also the separation of fact and fiction. Our...depends on it. Deanna McLafferty is an editorial assistant and activist. Washington, DC, USA 38.9072°N 77.0369...

...in an open inactive state, a closed active state, and an artificially active state due to closure by FP...long distance runner, gardner, and cook.   References 1. Snapp, E.L., et al., Formation of stacked ER cisternae...you are using a tag with a serious drawback. The standard EGFP plasmid that used to be sold by Clontech ...its molecular affinity, termed the dissociation constant or Kd, and its concentration. The smaller the ...membranes together and can wrap the membranes into stacked structures including organized smooth endoplasmic... to a dimerizing FP has potential to form large stable polymers of dimers. 3) FRET biosensors that contain...of tubules can be grossly distorted into dense stacked membranes termed Organized Smooth Endoplasmic Reticulum...

... worked from Standard Operating Procedures (SOP’s) and every item I tested was constantly tracked with...touch with my colleagues and have misunderstandings.  Working long distance, you need to be very self-motivated...people from another background or culture could understand. I did, however, find it very hard to speak with...to be in the lab doing experiments  without the constant fear of failure because I knew the tests worked...the different tests and became efficient, I then started to become bored because I was not being mentally...mentally challenged. For these and other reasons, I started  looking for other career options after 6 months...redundancies can be made within the company; new staff hired in anticipation of the contract suddenly have...

...always a good idea to start close to your comfort zone and slowly expand from there. Start small: Collaboration...implementation, and now must focus on managing the established partnership. If you’re willing to do similar ...my graduate studies at Karolinska Institutet I started working with other groups. First within my department...example, I worked with some microscopy enthusiasts to start a microscopy seminar to connect the imaging community... scientific collaboration - show initiative and start organizing meetings and take minutes. Get to know... little in common with you is much harder than starting a collaboration with a colleague. However, reaching...discussing with strangers stopped feeling weird and started to be fun. I just had to remember to approach the...

... job and job requirements. It’s okay to start from a standard version, but take some time to make it fit...your application, but it can certainly help you stand out.  In some fields, a cover letter is vital, particularly...a good idea to talk to people in the field to understand what the norms are. What is a cover letter for... to master ddPCR.  Finally, it’s a good idea to state why you’re applying to the specific company or lab...struggling to think of what to put in your cover letter, start by looking at the requirements in the job description...rationale for designing it the way you did, and state that it would help you manage a large number of ...yourself and your background. It’s a great place to state why you’re applying to the job, company, and/or ...

...high school to college to graduate school, standing at the start of a science policy career was much more...for science policy folks is on Twitter- start an account and start engaging with the #scipol hashtag. Often....  Who does science policy? I’ve intentionally started with the ‘who’ question because the science policy... from your alumni community is a great place to start. What is science policy? THE IMPOSSIBLE QUESTION...branch or in Congress, or can also happen at the state and local level. The big secret about science policy...tenured professors. There’s never a bad time to start your science policy journey.  In fact, working in...your career through engagement with your local or state governments. Most communities have opportunities...

...biology, innovative new imaging approaches are constantly being developed which combine computational processing...this. This recent push gives users the chance to understand the "black box" provided by the manufacturers.... 1: Open source hardware builds   Since I started my PhD, I have been convinced that most microscopy...creativity and feedback from the community to create state-of-the-art devices for research. There are a number...support the community in developing better, more sustainable, easily sharable products. Open-Source Microscopy...time-lapse images, quantitatively evaluating antibody staining, or counting cells are now routine, open-source... process framework FIJI are just three of the constantly growing number of "community-driven" software...

...Challenge #2: Technical obstacles to plant synthetic biology In addition to public resistance, an overall limited...limited understanding of plant biology is a significant technical obstacle to plant synthetic biology. We.... Read their previous blog post about how they started their company here. The first transgenic plant ...technology. Monoculture and chemical spraying, mainstays of modern agriculture, are now synonymous with...imagine any use for GM technology other than to sustain those techniques. We feel strongly that the scientific... We cannot build what we do not understand, and the complicated and unique metabolic processes of plants...project in a field with 30 years of basic research standing behind it. Our color changing flower involves ...

...bind DFHBI, is highly unstable in vivo due to poor folding and thermal instability at 37°C (Ouellet et ...to the instability of Spinach, and were able to develop an aptamer that was more thermally stable, exhibited... not photostable, and were presented in the study to support the uniqueness of the photostability of the...) and requires a tRNA scaffold to improve its stability in vivo. However, Spinach has proven useful in... RNA. Spinach-based riboswitches can be used as stable metabolite sensors for imaging in live cells. By... fluorescence. In the search for something more stable for quantitative imaging, Ming Hammond’s group ...lower dependency on magnesium and higher thermal stability, making it a potentially more useful tool for ...

...imaging is an easy way to understand what is going on in the body at steady state or under physio-pathological...adult organisms, where tissues structures are more stable and local movements predominate (e.g. a basal progenitor...progenitor cell migrating to the epithelium). At this stage, only cells from the immune system show wide scale...information regarding immune cell mobility than the static microscopy images of chemically coloured lymphoid...immunologists the ability to count cells and statistically analyze cell movements. By using cells labelled...two-photon microscopy allowed scientists to better understand T cell initiation in the adaptive immune response...uncovered host-pathogen interactions leading to the understanding of effector function in infected tissues. Before...

...distribution status. Hold for publication: we will initiate our MTA process and perform standard quality ...DH5α, NEB Stable, or ccdB Survival.­ Whenever possible, we propagate plasmids in the standard cloning strain... need to create an account with Addgene before starting the process. After you have logged in to your ...article information You have three options when starting the deposit: Figure 2: Choose to submit published...manuscript is published in a peer-reviewed journal. To start this process, simply enter your preprint title or...available for distribution as soon as they pass our standard QC process and MTA has been approved. You can...considered large deposits and are exceptions to our standard workflow.) Our spreadsheet contains columns that...

...for you, don’t wait. Start communicating now. These 9 strategies will get you started:  Subscribe to Career...Take the DIY approach and start a blog. Stellar talents like Ed Yong jumpstarted their science writing career...Twitter account. Check out the hashtag #scicomm and start following science communication societies like @...local pub at a Pint of Science event. Or get on stage for a Science Slam – a sort of competitive TED talk..., set up a blog using a site like WordPress and start writing. Grow your audience by advertising your ...are open to guest contributions. If you decide to start your own blog, check out these two excellent articles... is the path you want to follow, check out the distance learning courses at the National Council for the...

... The STAR interview method is a popular way to answer behavioral interview questions. STAR stands for ... describe all four parts of a STAR answer once the interview has started. Instead, I preferred to prepare...was my experience not long ago. I just recently started working in the pharmaceuticals industry after earning...several individual and/or small groups, usually starting with the hiring manager. These interviews lasted...Story telling shares many of the same elements as a STAR answer: a setting, a conflict, and a resolution....which is something not inherently captured by the STAR method. I like to think of the theme of my interview...resumes are like cliff notes, so I use these as a starting place for drafting my stories. In total, I prepare...

...antibody to stain a target protein in mouse tissues, causes a high degree of background staining. This occurs...secondary antibody) approach?  Staining for multiple targets When staining multiple targets indirectly, ...for a four-antibody panel, staining in parallel    In short, when staining multiple targets, look for...validated for your application, of course!) When the stars are aligned and the lab gods are smiling down at...the tissue sample. There are species-on-species staining protocols that use antibody fragments to block...endogenous immunoglobulins and reduce the background staining but they do not always eliminate the issue (Lu...better to choose primary antibodies from more distantly related species and/or use narrowly specific secondary...

...to gain support for CINSO. For instance, I tweeted to the Indiana State Museum and others when I was trying...engaging the public to promote an awareness and understanding of science. Like most science PhD programs, ...often called “nontraditional,” helped prepare me to start CINSO. Here, I share some of the tips I learned ...up and moved on to the next thing. If I made a mistake, I evaluated what lessons I had learned – call ...the process. As I mentioned, my graduate office staff was amazingly supportive. I talked to them about... had to take advantage of it. Blog and tweet I started my blog, Born to Science, as a graduate student...experience with my blog helped immensely when CINSO started brainstorming the resources we wanted to provide...

...Purdue University and is working to understand Lowe Syndrome, a devastating genetic disease caused by mutations...developing metastatic melanomas and other cancers. Joshua Wang (bottom right), also of the Wistar Institute... patients with the condition to reveal some “outstanding differences.” They now have some theories on ...behavior in greater detail. The goal is not just to understand the disease better but hopefully also to find...Rare Genomics Institute and Addgene will make a substantial difference in pushing the work forward. Xeroderma...Hendrik De Buhr (top right) and his colleagues at the Wistar Institute in Philadelphia, PA will put their award...the lab got interested in XP out of a desire to understand the early events in the transformation of healthy...

...schools and was started in 2005 in Kansas by Bobby Henderson, a physics graduate from Oregon State University...through synaptic connections (Shepherd, 2004). Understanding the structure and function of these neural circuits...researchers often want to do at neuronal synapses for instance. Also these tags need to be fused to scaffold ...scaffold proteins in order to be expressed stably in neurons. As compared to epitopes tags, FPs are intrinsically...circuits and dynamics. FPs are generally bright, stable and well tolerated by cells. They can be used for...The issue with these FPs is that they are less photostable than GFP. In addition, antibody-based amplification...useful for multicolor imaging experiments than standard FPs. The Looger Lab was thus able to easily perform...

... its unbound state contains a fluorophore and a quencher in close proximity. For instance, the fluorophore...was less stable due its RNA nature, it paved the way to new biosensor possibilities. For instance, further...years ago, two independent groups, led by Jack Szostak and Larry Gold, developed methods for selecting... from experts on FRET techniques. By combining standard donor/acceptor FRET know-how with aptamers, aptabeacons...aptamer system. Knowing that GFP’s  structure stabilizes a 4-hydroxybenzylidene imidazolinone (HBI)-like...Spinach eventually evolved into Spinach2, a more stable and brighter version of the aptamer, but it still... of SELEX and FACS, Broccoli is inherently more stable in cells. The new aptamer also has a higher melting...

...regardless of visa or fellowship status, in addition to certain staff scientists and those in technical...$47,476 per year, under updates to the Fair Labor Standards Act (FLSA). This is having a major effect on ...students are exempted from the law. However postdocs, staff scientists and technicians in primarily bench-science...$43,692 to $50,000, an increase of 14.4%. In 2015, Stanford raised salary levels to $50,000, and as of October... The University of Notre Dame sets no minimum, stating that while they recommend NRSA levels, postdocs...not to be tracked. University of Buffalo and Ohio State both have minima of $30,000, and raising salaries...addition, both Brown and Brandeis universities have stated that they consider postdocs paid on stipends to...

...control copy number and stability, which influence the plasmid’s ability to establish and maintain itself ...Understanding the characteristics of natural plasmids and how they function in nature can inform us on...others have a broad host range and can replicate and stably maintain the genes they carry among organisms belonging... many different phylogenetic groups (Jain and Srivastava 2013).  Recombinant vectors that are made from...from broad host range plasmids are more stable, can be easily cloned in different bacteria, and can be ...transferred to various experimental hosts (Jain and Srivastava 2013). They are also easily-shareable tools within...utilize diverse host replication machinery. For instance, the ori typically contains iterons that serve...