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We narrowed to 610 results for: sam

Showing: 221 - 240 of 610 results

  1. Important Considerations When Using AAVs

    Type
    Blog Post
    ...chemogenetic receptors delivered by AAV within the same cell types. Not only can we compare these methods...McCarty, D. M., Paul E. Monahan, and Richard J. Samulski. "Self-complementary recombinant adeno-associated...PMC190186. 9. Grieger, Joshua C., and Richard J. Samulski. "Packaging capacity of adeno-associated virus...

  2. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...the power of the technique relies heavily on the same fluorophore being imaged multiple times in order...Additional PA-FP molecules are then activated in the same manner, until enough molecules have been analyzed...18727145. PubMed Central PMCID:PMC4007248. 11.Hess, Samuel T., Thanu PK Girirajan, and Michael D. Mason. "...

  3. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ... these regions, even if they are present in the sample. Other techniques, like electron microscopy and...RNA-bound state (PDB ID 4ZT0, bottom), seen from the same angle. Box highlights the DNA-binding region of ... structure (right, PDB ID 7S4X), viewed from the same angle to highlight conformational differences. HNH...

  4. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...multiplexed gRNAs? By expressing multiple gRNAs on the same plasmid, you’ll make sure that each cell that gets...situation: you only need to express two gRNAs at the same time. One system you could use is pX333 from the...options that are available from Addgene follow these same assembly principles, but they’re optimized for different...

  7. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...function like your average AAVs — and have many of the same restrictions, like limited cargo space (~4.7 kb)...systems, which use two or more AAVs to transduce the same cell, can accommodate larger, modular genetic payloads...efficient AAV-PHP.eB, developed and deposited by the same lab. AAV-PHP.eB is the most commonly requested systemic...off-target organs. AAV.CAP-B22 was developed through the same process as AAV.CAP-B10 and shows high transduction...

  8. AAV Packaged on Request is Here!

    Type
    Blog Post
    ...plasmids and your viral packaging service in the same place, making it easier to go from selection to ...control.  Our packaged on request AAV service uses the same trusted production and quality control processes...

  12. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    ...toolkit - aptly named GreenGate – offers all of those same advantages to researchers working in plant model...allows us to design modules that always have the same recognition site but distinct sites where the enzyme...

  13. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    ...N- and C-terminal. Each set of plasmids uses the same restriction enzymes for cloning (see schematic below...one need to remove the tag post-purification. A sample of pCri constructs: pCri-1a, -4a and -9a. 6xHis...

  14. Science as Art

    Type
    Blog Post
    ...could provide a new tool to study old and unique samples, such as those collected by Darwin on his voyage...incompatible with the need to conserve these unique samples, meaning that we know very little about these specimens...

  15. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    ...produce your Packaged on Request prep and run the same quality control assays we run with all of our in-stock... be 1x1013 and titers for AAV2 to be 7x1012, the same titers found in our in-stock preps. If a request...

  16. A Better Way to Get Customer Support: The Help Center

    Type
    Blog Post
    ...resources with our new Help Center! Created using the same reliable Zendesk system that powers our email support...support team’s attention, where we can then offer the same high level of direct service you’ve come to expect...

  17. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    ...wrong. Whether it be a bad fridge freezing your samples, or a dysregulated water bath boiling your experiments...researcher doing some experiments in Brazil can have the same issues as one doing experiments in France. We can...

  18. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    ...optogenetics in a mouse experiment. Viral delivery is the same in both cases, but activation depends on small molecule...repolarize faster.  Chemogenetics does not offer the same level of control over timing. Because chemogenetics...
Showing: 221 - 240 of 610 results