...that the CRISPR system was involved. Today, that same laboratory enjoys a successful edit rate of over...later it became quite clear that others thought the same. Since January 2013 there have been 33 CRISPR software...knowledge from laboratories employing CRISPR on the same organism could be used as a proxy for this last ...

...function like your average AAVs — and have many of the same restrictions, like limited cargo space (~4.7 kb)...systems, which use two or more AAVs to transduce the same cell, can accommodate larger, modular genetic payloads...efficient AAV-PHP.eB, developed and deposited by the same lab. AAV-PHP.eB is the most commonly requested systemic...off-target organs. AAV.CAP-B22 was developed through the same process as AAV.CAP-B10 and shows high transduction...

...Say hello to the new Addgene blog! Same URL, same great articles to help you with your experiments, but...

...Not all plasmid preps are the same. Before purifying a plasmid from a bacterial culture, it is important...nucleic acid and chemical contaminants, these DNA samples must also be entirely endotoxin free. Endotoxin...

...less and you might suspect contamination in your sample or consider asking your sequencing facility to ...Use a silica spin column for purification of the samples you send for DNA sequencing If your sequencing ...

...toolkit - aptly named GreenGate – offers all of those same advantages to researchers working in plant model...allows us to design modules that always have the same recognition site but distinct sites where the enzyme...

...N- and C-terminal. Each set of plasmids uses the same restriction enzymes for cloning (see schematic below...one need to remove the tag post-purification. A sample of pCri constructs: pCri-1a, -4a and -9a. 6xHis...

...could provide a new tool to study old and unique samples, such as those collected by Darwin on his voyage...incompatible with the need to conserve these unique samples, meaning that we know very little about these specimens...

...resources with our new Help Center! Created using the same reliable Zendesk system that powers our email support...support team’s attention, where we can then offer the same high level of direct service you’ve come to expect...

...another paper. A large fraction of readers cover the same ground again and again as they don’t save the insights...disease or therapeutic Side-by-side comparison of the same experiment from different cell lines/model systems...

...wrong. Whether it be a bad fridge freezing your samples, or a dysregulated water bath boiling your experiments...researcher doing some experiments in Brazil can have the same issues as one doing experiments in France. We can...

...optogenetics in a mouse experiment. Viral delivery is the same in both cases, but activation depends on small molecule...repolarize faster.  Chemogenetics does not offer the same level of control over timing. Because chemogenetics...

...that it takes to be a good scientist are not the same ones it takes to be a competent manager (much less...job satisfaction Being a good manager is not the same as not being a bad manager (see cartoon above – ...

... over.” And we sent the Addgenies home.  At the same time, as Addgene started dealing with this new reality...and keep new projects on track to succeed at the same time. I began approaching science funding organizations...

...phenotype typically emerges. Every three days, a sample of cells was collected for scRNA-seq analysis and...et al. "CellTag Indexing: genetic barcode-based sample multiplexing for single-cell genomics." bioRxiv...

...flex PSAM4 GlyR IRES EGFP Chimeric channels for neuronal manipulation 119742  AAV5  AAV SYN PSAM4 GlyR...

... Attend events for one organization or go to the same conferences/meetings over and over to get to know...volunteer). Naturally running into or working with the same people is often the easiest way to build relationships...

...point of reference, Addgene commonly prepares NGS samples using both pre- and post-amplification DNA and ...libraries, but any analysis script should return the same output: a spreadsheet of read counts. You can use...

...fragments containing the desired mutations. This same process can be used simultaneously for the generation...cassette, ready for transformation into yeast that same day.  The field of synthetic biology has led to ...

...cis-acting elements, which means that they are in the same DNA molecule as the gene that they package. In a.... M., Dobbins, A. L., Li, C., Hirsch, M. L., & Samulski, R. J. (2020). Adeno-Associated Virus Serotype-Specific...