...ways to deal with this issue. One way is to grow multiple bacterial cultures of your AAV transfer plasmid...aliquots to use for qPCR titering. This helps avoid multiple freeze/thaws of your larger aliquots of virus.... a few different concentrations of virus, or multiplicity of infections (MOIs), to determine the optimal...

...Clustal is our frequent choice for pairwise or multiple sequence alignments of nucleotide or protein sequences... sequences. We also use COBALT for aligning multiple protein sequences, particularly for comparing different...//www.ebi.ac.uk/Tools/webservices/#multiple_sequence_alignment_msa http://www.ebi.ac.uk/Tools/webservices...

...microscope. You may be wondering why there are multiple objective lenses. Each objective performs essentially...determine the final magnification of your image, multiply the magnification of your objective lens by the...

...libraries utilize an engineered Cas9, which contains multiple point mutations in the nuclease domain. These ...affected by gene activation rather than depletion. Multiple strategies have been developed to recruit transcriptional...domain such as VP16 to dCas9 or the recruitment of multiple copies of VP16 termed the “Sun Tag” method (Tanenbaum...

...powerful imaging technologies, the combination of multiple fluorescent protein sequences to develop diverse...individual plasmids and are each flanked by the same multiple cloning site (MCS). Modules are assembled one ...not suited to generate polycistronic containing multiple identical polyadenylation signals or promoters...

...exponential decay process we observed, while 𝜓z(t) multiplies the likelihood by the corresponding factor 𝜙...parameter. We approximated the data likelihood with multivariate Gauss-Hermite quadrature, since the analytical...intractable integral of the product of a Poisson and multivariate log-normal likelihoods. Effects of weekends...

...individual genes can be relatively laborious because multiple plasmids/viruses are needed to deliver all the... then packaged into AAV for in vivo delivery. Multiplex gene targeting is also possible and is only constrained...vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR–dCas9-activator...

...Feng Zhang's group previously packaged Cas9 and multiple gRNAs into separate AAV vectors, increasing overall... AHM, Zhu K, Wagers AJ, Church GM (2016) A multifunctional AAV–CRISPR–Cas9 and its host response. Nat ... Wu X, Jiang W, Marraffini LA, Zhang F (2013) Multiplex Genome Engineering Using CRISPR/Cas Systems. Science...

...polycistronic tRNA-gRNA (PTG) gene system to clone multiple protospacers which enables us to target more than...Minkenberg, and Yinong Yang. "Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing...PMC5972399. 12. Molla, K., Shih, J. & Yang, Y. Multiplex CRISPR-mediated base editing in rice. In Genome...

...maintain itself within host cells.  The plasmid has multiple ori that are functionally differentiated from ...proteins from bacterial hosts. In a plasmid with multiple oris, one may function in one type of host and...only one ori and a broad host range plasmid with multiple oris that vary in DnaA-box and iteron structure...

...different viral families and can be used to target multiple delivery systems, such as lentivirus and MMLV ...recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. eLife, 8, e43322. doi... Multiple Authors...

... not, multiple techniques are used as part of a treatment plan, an approach known as multimodal therapy...are some genetic factors involved. For example, multiple studies show that certain mutations of the BAP1...

...et al., 2019). Because of its ability to target multiple gene loci simultaneously, CRISPRa can also be ...(Chavez et al., 2015)) are good choices across multiple cell lines (HEK293T, MCF7, U2-OS, Hela, N2A, 3T3...Church G (2016) Comparison of Cas9 activators in multiple species. Nat Methods 13:563–567 . https://doi....

...location in your recipient plasmid (usually in the Multiple Cloning Site (MCS)), but do not cut elsewhere ...Annealed-oligo Cloning . If you are lucky enough to have multiple options for enzymes that flank your insert and...

...assembly is well known for allowing easy assembly of multiple linear DNA fragments, but can also be used in ...biology, mainly because of the ease in assembling multiple fragments in one step with no scar sequences remaining...Whether it is for routine cloning, assembly of multiple fragments, or synthetic biology, you should consider...

...hotspot for dystrophin, exons 45-55, there are multiple common deletions that maintain the protein’s reading...skipping? For such a therapy to be successful, multiple requirements must be met. First, CRISPR would ...Thakore PI, Majoros WH, Reddy TE, Gersbach CA. Multiplex CRISPR/Cas9-based genome editing for correction...

... added to the reaction for easy detection. The multiple biotin binding sites found on avidin are also ...during PCR using a biotin labelled dUTP. There are multiple types of biotin labels that can be added to an...Biotin labelled oligonucleotides can be used in a multitude of applications including but not limited to in...

...with different ITAM/SH2 reporter pairs allow multiplexed measurement of different RTKs. Although this ...for lactate developed using a high-throughput multiparameter screen (Koveal et al. 2022). Lactate is a key... here!   Koveal D, et al. A high-throughput multiparameter screen for accelerated development and optimization...

.... Digestion with enzyme “B” detects primer multimerization (purple) which is evident by an increase in...CRISPR-induced genome modifications. Occasionally, multimerization of the PCR primers can cause duplication of...particularly relevant in cases where a large plasmid with multiple functional regions is used as template for the...

...is necessary or desirable to control or look at multiple genes at the same time.  The 3 binary systems ...analysis in Drosophila or for precise control over multiple genes in synthetic systems. Recent work has even... Multiple Authors...