Addgene

We narrowed to 271 results for: mel

Showing: 221 - 240 of 271 results

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Rett Syndrome: A History of Research and Therapeutic Outlooks

Type

Blog Post

... syndrome gene therapy improves survival and ameliorates behavioral phenotypes in MeCP2 null (S51.002)...
To Codon Optimize or Not: That is the Question

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Blog Post

...gene of interest? Codon optimization has become extremely useful in expressing functional proteins in hosts...
Getting the Most from Your Lentiviral Transduction

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Blog Post

... yeast, fungi, or bacteria, mycoplasma can be extremely hard to detect with levels reaching 108 cells ...
Multiple Plasmids at a Low Price: Kits from Addgene

Type

Blog Post

... Huang, Shijie, Xuechen Zhu, and Charles E. Melançon III. "Detection and Quantification of Ribosome...
Hot Biosensors 2022: Year-End Roundup

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Blog Post

...acssensors.1c02201. LiLac sensor for lactate Stop and smell the flowers - or rather, the lactate! The latest...
CRISPR 101: Validating Your Genome Edit

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Blog Post

...This post was originally written by Melina Fan and updated Nov 3, 2022 by Susanna Stroik. You’ve designed...
Antibodies 101: Normalization and Loading Controls for Western Blots

Type

Blog Post

...detection range, band intensity measurements become extremely susceptible to error, since tiny changes in intensity...
Hot Plasmids: Summer 2024

Type

Blog Post

...Adriaens, C., Ramadoss, G. N., Shi, Q., Hung, K. L., Samelson, A. J., … & Weissman, J. S. (2021). Genome-wide...
Antibodies 101: Introduction to Gating in Flow Cytometry

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Blog Post

...of my previous experiments where I harvested a melanoma tumor from a C57BL/6 mouse and analyzed the tumor-infiltrating...
An Integrin Antibody Toolkit from IPI

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Blog Post

...This pocket-binding property in an antibody is extremely rare. While many function-blocking integrin antibodies...
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

Type

Blog Post

.... In fact, for several FRET biosensors (yellow cameleon 3.60, EPAC, and Galphai), the C-terminus and N-terminus...
Plasmids 101: Biotinylation

Type

Blog Post

...molecule of biotin. Biotin and avidin have an extremely strong affinity for one another. Actually the ...
Transferable Skills Guide: Conflict Resolution

Type

Blog Post

...other just for the sake of disagreeing. It can be extremely toxic in a lab or work environment if left unchecked...
Site Directed Mutagenesis by PCR

Type

Blog Post

...introduced anywhere in the plasmid (albeit at extremely low frequency), and these could interfere with...
Evolution of Lab Techniques

Type

Blog Post

...and behavior. References 1. Southern, Edwin Mellor. "Detection of specific sequences among DNA fragments...
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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Blog Post

...CRISPR may be the way to accomplish this tough job! Kamel Khalili’s lab at Temple University has demonstrated...
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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Blog Post

...recommends an overlap of 15-40 bp, with a primer melting temperature greater than 48℃. Both Snapgene and...
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

Type

Blog Post

...labeling for neural circuit analysis in Drosophila melanogaster. Hadjieconomou D, Rotkopf S, Alexandre C, Bell...
A Control for All Seasons

Type

Blog Post

...2009). If the fluorescence from your target is extremely bright, then the noise likely will not interfere...
Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

Type

Blog Post

...Miura et al. (2015) developed the ivTRT method to ameliorate this problem. Briefly, you design your donor ...

Showing: 221 - 240 of 271 results

Results per page:

Rett Syndrome: A History of Research and Therapeutic Outlooks

To Codon Optimize or Not: That is the Question

Getting the Most from Your Lentiviral Transduction

Multiple Plasmids at a Low Price: Kits from Addgene

Hot Biosensors 2022: Year-End Roundup

CRISPR 101: Validating Your Genome Edit

Antibodies 101: Normalization and Loading Controls for Western Blots

Hot Plasmids: Summer 2024

Antibodies 101: Introduction to Gating in Flow Cytometry

An Integrin Antibody Toolkit from IPI

Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

Plasmids 101: Biotinylation

Transferable Skills Guide: Conflict Resolution

Site Directed Mutagenesis by PCR

Evolution of Lab Techniques

Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

A Control for All Seasons

Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

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