...indicating loci where SNPs reside on sgRNA target sequences for sgRNAs that target ancestry-associated genes...that could analyze CRISPR sgRNAs and report the likelihood of any given sgRNA having an ancestral bias....

...activity coupled with a customizable single guide RNA (sgRNA) that bears complementarity to a 20bp target sequence...multidirectional control, a new study repurposes the sgRNA to include modular synthetic RNA scaffolds that ...modules are comparatively small allowing multiple sgRNA constructs to be introduced simultaneously in a ...

...endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA expression yielded editing of 80–90% in human cell...limited (likely due to its long, negatively charged sgRNA), RNP delivery was much better with Cas12, which...Workflow to assess Cas9-PAGE editing with viral sgRNA targeting mCherry reporter (mChe), with co-incubation...

...including the cell line/tissue type, enzyme system, sgRNA library and other details relevant to screen result...gene-level scores provided by authors rather than sgRNA-level sequence read scores. The original supplemental...

...This ‘Caspex’ construct is guided by a pre-defined sgRNA sequence to a specific genomic locus and labels ... and targeted to a sequence of interest with an sgRNA expressed from a separate plasmid. When cells are...

...shRNA sequence specific to your gene’s mRNA or a sgRNA targeting an exon in your gene of interest, for ...with many applications. These libraries include sgRNAs targeting the entire genome, overexpression of ...

...lentiviral expression vector, which contains an sgRNA insert site and a reporter for tracing the engineered...editing tool on the immune system, the group inserted sgRNAs targeting various innate and adaptive immune genes...

... the BFP variant, a BE4am-Cas9 base editor, and sgRNA vectors for the BFP-to-GFP conversion and to target...cytidine base editor. The lab then introduced an sgRNA targeting the GFPACG to the cells. As expected, ...

...those generated by multiplexed delivery of multiple sgRNAs. Since multiplexing is commonly used to create ...helps the researcher see which of the multiplexed sgRNA was involved in a particular edit, and which type...

... day of CRISPR, Addgene gave to me: engineered pegRNAs to improve prime editing efficiency. On the third...

... protein is 1,241 amino acids long, has optimal sgRNA spacer length of 22-25 nts, and is thermostable ...

...3xFLAG-tag fusion proteins. The repair template and Cas9-sgRNA plasmid is then injected into the germline of young...

...impact of off-target mutations, scientists are using sgRNA for selection and optimization. In addition, the...

...γ-Retroviral transfer plasmid encoding a transgene, sgRNA, or shRNA of interest: The transgene sequence is...

...genomic locus in any cell type. By varying which sgRNAs are used with FIRE-Cas9, experiments can be designed...

...of PE protein components (Figure 1), an altered epegRNA delivered with an enhanced pU6cm promoter, viral...

...protocol listed under “Supplemental Document”: gRNA_Cloning Vector (deposited by George Church’s lab). Addgene...

... Using an elongated template + guide RNA called pegRNA (prime editing RNA), the prime editor can install...

...example, sgRNA-A’s protospacer sequence and PAM happen to fall on the bottom (Crick) strand while sgRNA-B’s...primers flanking each sgRNA target site (within 150 - 350 bp) to amplify the sgRNA target site to examine...complement: 1.0 μl sgRNA 24- or 25-mer oligo (100 μM; see step 1.4 ), 1.0 μl sgRNA 24- or 25-mer reverse...composed of a polyclonal population exposed to sgRNA-A and sgRNA-B (see step 5 ). The purpose of the following...delivery of a pair of chimeric single guide RNAs (sgRNAs) to create two double strand breaks (DSBs) at a...posted at the top of the page. CRISPR Design Design sgRNAs manually or using freely available online tools...non-coding elements. For creating a gene knockout, two sgRNA located within exons will enrich even monoallelic...

...Luciferase Type Promoter Description PI 60226 AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Renilla...