...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...collection that contains 1,836 genes encoded by 3,548 isoforms and two control vectors. The individual clones...purchased at Addgene. Each transcription factor isoform has a unique 24 bp barcode that simplifies identification...identification of the transcription factor isoform in pooled screens. This collection and library can be...transcription factor open reading frames. Each isoform is uniquely barcoded with a 24 bp sequence, to ...

...proteins produced by bacteria become insoluble, forming inclusion bodies that are difficult to extract ...expression vector that is then transformed into a bacterial cell. Transformed cells propagate, are induced...post-translational modifications including protein folding, formation of disulfide bridges, glycosylation and acetylation...

...monoclonal antibodies (mAbs) and their recombinant forms (rAbs). mAbs and rAbs have higher homogeneity than...non-target proteins), variability (separate batches can perform differently – happens when the antibody is produced...binding to off-target molecules), reliable (wouldn’t perform differently over time), compatible with the desired...images similarly to the negative control, that informs us that the primary antibody is very weak or with...the hippocampus compared to other regions, that informs us that this antibody has specific binding and ...

... by blogging. Writing a blog is not only a great form of scientific outreach, it also helps you to hone...talk – to win the audience’s applause with your performance. When your institute opens its doors to the public...cover their annual meetings. 8) Training – Get a formal education Some people get into science communication... Get your community involved in sampling or bioinformatics analysis or upload your data to Zooniverse....

...cells. Now, Francois St-Pierre’s lab developed a new form of FACS-based cell screening, Single-cell Phenotypic...optogenetics. Optogenetic tools use light to induce conformational changes in a protein.    Cells expressing...reduced scale in screens without any reduction in performance compared to larger genome-wide libraries. Find...

...not occur or be detected even if the two proteins form a complex. To help troubleshoot your experimental...protein and are useful when a process affects the conformation of the probe. More on these biosensors later...would further increase the amount of correlated information available from a cellular process. FRET experiments...

...cautioned the distinction of “higher” and “lower” life forms as we shared millions of years of common ancestry...findings from structural studies on the ribosome performed in his lab. RNA is a structural and functional...that RNA in the ribosome is in a complicated conformation “not like DNA which has a boring structure.”...

...are two fascinating examples of epigenetics in the form of imprinting. In imprinting, one copy of a gene...

...problems that need to be resolved. If there are any forms that need to be signed, or manuscripts that need...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this... RNA degradation seen in bacteria. Want more information on the wide variety of Cas enzymes? CasPEDIA ...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...

... gene it can be stuck in a constitutively active form, constantly activating pathways for cell growth ...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this... below. For proteins with multiple subunits or isoforms, individual links to each gene page are provided...gene. For components with multiple subunits or isoforms, individual links to each gene page are provided...E2F transcription factor ECT2 Epithelial cell transforming 2 EGFR Epidermal growth factor receptor EIF4EBP... SHC4 SHC (Src homology 2 domain containing) transforming protein SHOC2 SHOC2 leucine-rich repeat scaffold...Esposito Lab for use with the Gateway® Cloning Platform (Life Technologies™) to permit construction of...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...

... of interest is not available in a pure form, you can perform relative quantification, which measures ...If your protein of interest is available in pure form, you can use it to create a standard curve on your...different than the validation assays, we recommend performing your own validation assays. If you’re using previously...chemical-based. Before selecting one, check your antibody information. Many antibodies will have a recommended blocking...temperature for 1–2 years and are available in formulas specific to your choice of detection methods. ...Aldridge et al., 2008).  Woah! That was a lot of information to cover. But don’t worry — the next time you...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this... our guides to find the plasmids and science information you need before starting your experiment. Genome...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...a much lower efficiency than NHEJ. Want more information on the wide variety of Cas enzymes? CasPEDIA ...