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We narrowed to 445 results for: crispr

Showing: 221 - 240 of 445 results

  1. CRISPR References and Information

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    Collection
    ...Resources CRISPR Protocols Addgene CRISPR Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids...screening. CRISPR eBook Addgene's downloadable eBook about all things CRISPR. Addgene CRISPR blogs CRISPR tips... CRISPR References and Information CRISPR References and Information Addgene Resources CRISPR Software...inhibition, and barcoding. CRISPR Kits Addgene's collection of CRISPR plasmid kits. CRISPR Software Sanger Indel... curated list of CRISPR resources and references, including gRNA design tools, CRISPR FAQs, and community...plasmids. CRISPR Guide Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library...published on the Addgene blog. CRISPR Pooled Libraries Addgene's collection of CRISPR pooled libraries for large-scale...

  2. CRISPR Pooled gRNA Libraries

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    ...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR is ... CRISPR Pooled Libraries CRISPR Pooled Libraries Browse CRISPR Plasmids By Function...experiments using a pooled CRISPR library are far more complex than using CRISPR to modify a single genomic...steps for using pooled CRISPR libraries and keep the following in mind: CRISPR screening experiments require...Interferon-Stimulated Gene CRISPR Knockout Library 125753 Knockout Human Emerman OhAinle 3rd 8 15,416 Human CRISPR lncRNA ...18,343 Human UBDUB CRISPR Knockout Library 171531 Knockout Human Li 3rd ~6 9,274 MYC-CRISPR Library 173195...Human CRISPRi Non-coding Libraries 86538 — 86550 Inhibition Human Weissman 3rd 10 Varies CRISPR/Cas9-assisted...

  3. Design Tips for Prime Editing

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    Blog Post
    ...Download the CRISPR 101 eBook Resources on Addgene.org Read the CRISPR guide Find CRISPR plasmids by ...editing guide RNA (pegRNA). The pegRNA consists of a CRISPR gRNA extended to include a primer binding site ...Prime Editing: Adding Precision and Flexibility to CRISPR Editing PRIDICT: Predicting Efficiencies of Prime...

  4. Lentiviral Vector Uses and Overview

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    Blog Post
    ...Lentiviral vectors are the vector of choice for many CRISPR applications, and they’ve also had success in clinical...transgene-expressing or knockout cell lines. Many CRISPR plasmids are designed for lentiviral use, including... Improved vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nat Methods...Lentiviral Plasmid FAQs Answered Learn How Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening...Screening Read about Genome-Wide Screening Using CRISPR/Cas9 Additional Resources on Addgene.org Check out...including Addgene’s top requested plasmid in 2015: lentiCRISPR v2, used both for targeted modification and genome-wide...

  5. CRISPR Guide

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    ...Guide CRISPR Guide Jump To: CRISPR Guide Overview CRISPR Basics Small Edits Large Edits Genome-Wide Screening...Figure 7: Overview of CRISPR transposases Browse Plasmids: CRISPR transposases CRISPR Recombinases and Integrases...screening using CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries...References Website CRISPR plasmids and resources at Addgene CRISPR 101 eBook (3rd edition) CRISPR software and... Addgene Blog CRISPR topic page How to design your gRNA for CRISPR genome editing CRISPR 101: Homology.... Figure 1: Overview of the basic CRISPR mechanism Engineered CRISPR systems contain two components: a...generating gRNAs makes CRISPR one of the most scalable genome editing technologies, making CRISPR perfect for genome-wide...

  7. Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids

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    ...Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids You may also like... CRISPR Guide CRISPR Protocols... Genome Engineering CRISPR Joung Lab CRISPR Plasmids...Tools CRISPR Blog Posts RNA-Guided Nucleases ( RGNs ), based on naturally occurring Type II CRISPR-Cas ...variant) Engineered CRISPR-Cas9 nucleases with altered PAM specificities Dimeric CRISPR RNA-guided FokI Nucleases... Nucleases (RFNs) CRISPR-Cas/RGN expression plasmids for use in human cells CRISPR-Cas/RGN expression ...information about RGNs and CRISPR-Cas systems can also be found at http://www.crispr-cas.org . Users are also...Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids Genome...

  8. Why and how to start studying chromatin (with Addgene's help)

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    Blog Post
    ...Chromatin Interactions: Using CRISPR-dCas9 to Study Gene Regulation CRISPR 101: Epigenetics and Editing...Studying Protein-DNA Interactions CRISPRainbow and Genome Visualization CRISPR Between the Genes: How to Experiment...or DNA repair, or replication, or any number of CRISPR applications and thought “OK, but how does this...

  9. Bikard Lab - CRISPR Repression Collection

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    ...Lab - CRISPR Repression Collection Bikard Lab CRISPR Repression Plasmids You may also like... CRISPR Guide...Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The David Bikard Lab at the Institute Pasteur...a plasmid‐borne CRISPR array coding for guide RNAs (diamonds) interspaced with CRISPR repeat motifs (squares...From the Bikard Lab, CRISPR plasmid collection for modulating expression levels of two targets independently...Pasteur has deposited a collection of CRISPR arrays to modulate the expression levels of two targets independently... DNA-binding protein dCas9 is expressed along a CRISPR array that contains 20 bp "addresses", where dCas9...collection comprises 20 plasmids, each carrying two CRISPR guides. The first one binds to sfgfp with either...

  10. PiggyBac-ing Through the Genome Editing Field

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    Blog Post
    ...piggyBac™ Transposon Catch up on Everything CRISPR with Our CRISPR Guide Deposit Your piggyBac™ Vectors! ... to the development of site-specific nucleases (CRISPR/Cas9, TALENs, and ZFNs). However, several challenges...piggyBac™ transposon system can be combined with CRISPR/Cas9 to efficiently perform genetic manipulations...interest, along with a site-specific nuclease (such as CRISPR/Cas9) to create a double-stranded DNA break. After...technologies including Zinc Fingers, TALENs, and CRISPR-Cas9 nucleases. Mathew applies his specialized ...

  11. Worm Expression Resources

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    ... individually. CRISPR Read Addgene's CRISPR Guide for a general introduction to CRISPR technology. Addgene's... tag insertion using CRISPR/Cas9 and the selection of C. elegans strains. CRISPR plasmids from John Calarco's...genome editing in C. elegans via a CRISPR-Cas9 system. Cloning-free CRISPR for C. elegans , which uses purified...Browse plasmid tools, kits, and resources (such as CRISPR and synthetic biology collections) for use in C... feeding worms bacteria expressing dsRNA or use CRISPR/Cas9 gene editing to easily engineer specific transgenic...contain tools for use in worm research. This includes CRISPR, RNAi, empty backbones, fluorescent proteins, and...Addgene's worm CRISPR resources include plasmids expressing Cas9, empty gRNA backbones, and constructs ...

  12. CRISPR Plasmids - Parasites

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Eukaryotic... CRISPR Parasites CRISPR Plasmids... Libraries Toxoplasma CRISPR Knockout Pooled Library - Toxoplasma gondii CRISPR genome-wide knockout gRNA...CRISPR expression plasmids that have been designed for use in parasites. CRISPR...Plasmids: Parasites Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional... research for decades. Recent developments with CRISPR-Cas9 gene editing have propelled both reverse and... forward genetics of these parasites. Moreover, CRISPR-dCas9 gene regulation has also been established...

  13. CRISPR Plasmids - Cascade-Cas3

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Cas3... CRISPR Cas3 CRISPR Plasmids: Cascade-Cas3...Find plasmids for CRISPR-Cas and Cascade for large genomic deletions. CRISPR...Cascade-Cas3 Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional... Cas3 is a unique member of the CRISPR family. After nicking the target DNA, Cas3 initiates single-strand...break, either due to a boundary defined by anti-CRISPR proteins or running into other proteins bound to...mechanisms. Cas3 belongs to the Class 1 family of CRISPR systems, the most abundant type found in bacteria...

  14. CRISPR Plasmids - Tagging

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... CRISPR Protein Tagging CRISPR Plasmids...Read our CRISPR 101 post for more information about HDR/repair template design. How to use CRISPR to tag...for a CRISPR-based system to add a tag (currently using FLAG) to endogenous proteins. This CRISPR/Cas plasmid-system...SapTrap CRISPR/Cas Toolkit SapTrap is a modular toolkit from Erik Jorgensen's lab for building CRISPR targeting...CRISPR plasmids for endogenous tagging of your gene of interest. CRISPR...Plasmids - Protein Tagging Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit...

  15. Plan Your Experiment

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    ... Science Guides CRISPR Guide Plan Your Experiment Plan Your CRISPR Experiment Jump To: Plan Your Experiment...you started using CRISPR in your research. Although we will use the example of CRISPR/Cas9 in mammalian...express the CRISPR machinery. For more difficult cells (e.g. primary cells), viral delivery of CRISPR reagents...conducting genome-wide screens using CRISPR AAV transduction CRISPR elements are inserted into an AAV transfer...lipids Transient expression of CRISPR components Short window of CRISPR activity may decrease off-target...Expression System Design gRNA Publications Get Started CRISPR is a powerful system that enables researchers to... cells, many of these principles apply to using CRISPR in other organisms. First, consider the genetic...

  16. CRISPR Plasmids - Purify Genomic Loci

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Identifying... CRISPR Purify CRISPR: Purify Genomic ...Catalytically inactive CRISPR plasmids for purification of target loci. CRISPR...Genomic Loci Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...essential to understanding locus function. Using CRISPR, researchers have expanded chromatin immunoprecipitation... conventional methods for genomic purification, CRISPR-based purification methods are more straightforward... with a genomic region of interest in vivo. For CRISPR-based purification, design your gRNA sequence to...

  17. CRISPR Plasmids - Epigenetics

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    ... CRISPR Epigenetics CRISPR Plasmids: Epigenetics Browse CRISPR Plasmids By Function...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts To...CRISPR plasmids for targeted epigenetic modification, including histone acetylation/demethylation, and...and are potentially heritable in dividing cells. CRISPR Activate and Interfere technologies also indirectly...enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity... . Browse, sort, or search the tables below for CRISPR plasmids for epigenetic modification in mammalian...

  18. Zhang Lab CRISPR Page

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    ... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang...Zhang Lab CRISPR Plasmids Available from Addgene Zhang Lab CRISPR Resources Tips for and FAQs about the ...Zhang Lab CRISPRs Browse All Zhang Lab Plasmids Zhang Lab Website You may also like... CRISPR Guide CRISPR...phages and plasmids. CRISPR loci in microbial hosts contain a combination of CRISPR-associated (Cas) genes...specificity of the CRISPR-mediated nucleic acid cleavage. We have harnessed the type II CRISPR nuclease system...backbone cloning information: CRISPR-Cas9 mouse toolbox protocol 637.8 KB CRISPR-Cas9 Cre expression vectors...CRISPR Cas9 gRNA plasmids from the Zhang lab Genome...

  19. Addgene: The First Twenty Years

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    Blog Post
    ...And when CRISPR arrived in 2012, our repository was rapidly able to support sharing of CRISPR constructs....org/guides/crispr/” — SirHamhands, responding to a request for “any suggestion for CRISPR online course...

  20. Plasmids 101: Modular Cloning

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    Blog Post
    ...assemble and test many variables all at once. CRISPR CRISPR arrays and libraries depend on many gRNAs delivered...etc. MoClo is especially useful for more complex CRISPR experiments (e.g., many guides/parts delivered)...delivered) in certain model organisms. In mammals, most CRISPR libraries are assembled and delivered virally, ...
Showing: 221 - 240 of 445 results